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Blue native polyacrylamide gel electrophoresis - a functional approach to plant plasma membrane proteome studies

Kjell, Jonas LU (2004)
Abstract
In this thesis, I have shown that a method called BN-PAGE (a kind of native gel electrophoresis) can be used for studying the protein composition in the plasma membrane of plants. The method enables the study of native (in the functional units) hydrophobic protein complexes, i.e., the membrane proteins and membrane associated proteins that form complexes in the cell. In the method, the protein complexes are first separated in a native dimension, then the componens of the complexes are separated.



The most abundant plasma membrane protein complexes could be mapped using BN-PAGE. These complexes are water channels, proton pumps, proteins that build cell wall resembling sugar polymers and proteins that probably contribute to... (More)
In this thesis, I have shown that a method called BN-PAGE (a kind of native gel electrophoresis) can be used for studying the protein composition in the plasma membrane of plants. The method enables the study of native (in the functional units) hydrophobic protein complexes, i.e., the membrane proteins and membrane associated proteins that form complexes in the cell. In the method, the protein complexes are first separated in a native dimension, then the componens of the complexes are separated.



The most abundant plasma membrane protein complexes could be mapped using BN-PAGE. These complexes are water channels, proton pumps, proteins that build cell wall resembling sugar polymers and proteins that probably contribute to maintaining the cell structure upon environmental stress. In the thesis, it is also shown how a change in the outer environment such as cold stress affect these protein complexes, as well as how salt treatment can affect the complexes. Yet another approach to the separation method is presented.



The method is one of few that enables the study of membrane proteins in functional units. Hopefully, the method can be more extensively used in the future, as it appears to be highly promising and at the same time not widely used today. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Thesis
publication status
published
subject
keywords
dodecyl maltoside, BN-PAGE, plant plasma membrane, protein complexes
ISBN
91-85067-12-1
language
English
LU publication?
yes
id
f12ebc56-b52e-494d-94ac-356a441a7dc3 (old id 526706)
date added to LUP
2007-10-08 12:24:46
date last changed
2016-09-19 08:45:17
@misc{f12ebc56-b52e-494d-94ac-356a441a7dc3,
  abstract     = {In this thesis, I have shown that a method called BN-PAGE (a kind of native gel electrophoresis) can be used for studying the protein composition in the plasma membrane of plants. The method enables the study of native (in the functional units) hydrophobic protein complexes, i.e., the membrane proteins and membrane associated proteins that form complexes in the cell. In the method, the protein complexes are first separated in a native dimension, then the componens of the complexes are separated.<br/><br>
<br/><br>
The most abundant plasma membrane protein complexes could be mapped using BN-PAGE. These complexes are water channels, proton pumps, proteins that build cell wall resembling sugar polymers and proteins that probably contribute to maintaining the cell structure upon environmental stress. In the thesis, it is also shown how a change in the outer environment such as cold stress affect these protein complexes, as well as how salt treatment can affect the complexes. Yet another approach to the separation method is presented.<br/><br>
<br/><br>
The method is one of few that enables the study of membrane proteins in functional units. Hopefully, the method can be more extensively used in the future, as it appears to be highly promising and at the same time not widely used today.},
  author       = {Kjell, Jonas},
  isbn         = {91-85067-12-1},
  keyword      = {dodecyl maltoside,BN-PAGE,plant plasma membrane,protein complexes},
  language     = {eng},
  note         = {Licentiate Thesis},
  title        = {Blue native polyacrylamide gel electrophoresis - a functional approach to plant plasma membrane proteome studies},
  year         = {2004},
}