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Real-time evaluation of antibacterial efficacy using bioluminescent assays for Pseudomonas aeruginosa and Staphylococcus aureus.

Patil, Manali LU ; Luo, Congyu LU ; Petruk, Ganna LU orcid ; Petrlova, Jitka LU ; Schmidtchen, Artur LU and Puthia, Manoj LU (2025) In Frontiers in Microbiology 16.
Abstract

The emergence of antibiotic resistance necessitates effective strategies for evaluating antimicrobial agents. Bioluminescent bacteria, either naturally occurring or engineered with modified reporter genes like bacterial luciferase, provide real-time assessment of bacterial viability through light emission. We investigated the antibacterial effects of cefotaxime and doxycycline using bioluminescent strains of S. aureus and P. aeruginosa, combining optical density measurements with bioluminescence monitoring. Treatment with cefotaxime resulted in a significant reduction of the bioluminescent signal in P. aeruginosa compared to untreated controls, while doxycycline induced a delayed growth curve. Both antimicrobials demonstrated strong... (More)

The emergence of antibiotic resistance necessitates effective strategies for evaluating antimicrobial agents. Bioluminescent bacteria, either naturally occurring or engineered with modified reporter genes like bacterial luciferase, provide real-time assessment of bacterial viability through light emission. We investigated the antibacterial effects of cefotaxime and doxycycline using bioluminescent strains of S. aureus and P. aeruginosa, combining optical density measurements with bioluminescence monitoring. Treatment with cefotaxime resulted in a significant reduction of the bioluminescent signal in P. aeruginosa compared to untreated controls, while doxycycline induced a delayed growth curve. Both antimicrobials demonstrated strong efficacy against S. aureus, as evidenced by decreased bioluminescence signals. Results from bioluminescence assays and classical minimum inhibitory concentration and minimum bactericidal concentration methods showed consistent alignment, validating the bioluminescence approach. This study demonstrates that bioluminescence-based methods offer a reliable, real-time alternative to traditional bacterial viability assays for evaluating antimicrobial efficacy.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Frontiers in Microbiology
volume
16
article number
1569217
publisher
Frontiers Media S. A.
external identifiers
  • pmid:40950597
  • scopus:105015432637
ISSN
1664-302X
DOI
10.3389/fmicb.2025.1569217
language
English
LU publication?
yes
additional info
Copyright © 2025 Patil, Luo, Petruk, Petrlova, Schmidtchen and Puthia.
id
5278ff5a-d509-40aa-9528-37353807add1
date added to LUP
2025-09-21 13:22:08
date last changed
2025-09-23 03:23:12
@article{5278ff5a-d509-40aa-9528-37353807add1,
  abstract     = {{<p>The emergence of antibiotic resistance necessitates effective strategies for evaluating antimicrobial agents. Bioluminescent bacteria, either naturally occurring or engineered with modified reporter genes like bacterial luciferase, provide real-time assessment of bacterial viability through light emission. We investigated the antibacterial effects of cefotaxime and doxycycline using bioluminescent strains of S. aureus and  P. aeruginosa, combining optical density measurements with bioluminescence monitoring. Treatment with cefotaxime resulted in a significant reduction of the bioluminescent signal in P. aeruginosa compared to untreated controls, while doxycycline induced a delayed growth curve. Both antimicrobials demonstrated strong efficacy against  S. aureus, as evidenced by decreased bioluminescence signals. Results from bioluminescence assays and classical minimum inhibitory concentration and minimum bactericidal concentration methods showed consistent alignment, validating the bioluminescence approach. This study demonstrates that bioluminescence-based methods offer a reliable, real-time alternative to traditional bacterial viability assays for evaluating antimicrobial efficacy. </p>}},
  author       = {{Patil, Manali and Luo, Congyu and Petruk, Ganna and Petrlova, Jitka and Schmidtchen, Artur and Puthia, Manoj}},
  issn         = {{1664-302X}},
  language     = {{eng}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Microbiology}},
  title        = {{Real-time evaluation of antibacterial efficacy using bioluminescent assays for 
        Pseudomonas aeruginosa and 
        Staphylococcus aureus.}},
  url          = {{http://dx.doi.org/10.3389/fmicb.2025.1569217}},
  doi          = {{10.3389/fmicb.2025.1569217}},
  volume       = {{16}},
  year         = {{2025}},
}