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Complete parameterization of temporally and spectrally resolved laser induced fluorescence data with applications in bio-photonics

Brydegaard, Mikkel LU ; Thompson, Alexander J.; Andersson-Engels, Stefan LU ; Bendsöe, Niels LU ; Svanberg, Katarina LU and Svanberg, Sune (2015) In Chemometrics and Intelligent Laboratory Systems 142. p.95-106
Abstract
We present a set of spectrally and temporally resolved clinical fluorescence data-with two separate excitation wavelengths-that was recorded in vivo. We demonstrate that data in the spectral and temporal domains are in certain ways coupled and provide a method for integrated and effective parameterization of spectrally and temporally resolved fluorescence (i.e., time-resolved emission spectra). This parameterization is based on linear algebra, matrix formulation and system identification. We demonstrate how to empirically extract single exponentially decaying components and provide rectified emission spectra without prior knowledge. We investigate the potential for improved cancer diagnostics according to the reduced parameters along the... (More)
We present a set of spectrally and temporally resolved clinical fluorescence data-with two separate excitation wavelengths-that was recorded in vivo. We demonstrate that data in the spectral and temporal domains are in certain ways coupled and provide a method for integrated and effective parameterization of spectrally and temporally resolved fluorescence (i.e., time-resolved emission spectra). This parameterization is based on linear algebra, matrix formulation and system identification. We demonstrate how to empirically extract single exponentially decaying components and provide rectified emission spectra without prior knowledge. We investigate the potential for improved cancer diagnostics according to the reduced parameters along the various domains. In this case, in terms of cancer diagnostics, we were unable to identify any benefits of simultaneously measuring both the temporal and spectral properties of the observed fluorescence. However, we note that this may be explained by an important experimental bias present in many studies of optical cancer diagnostics, namely, that, in general, suspected lesions always differ visually from the neighboring healthy tissue. (C) 2015 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biophotonics, Fluorescence lifetime, Optical spectroscopy, Dynamic, models, Population dynamics, Laser diagnostics
in
Chemometrics and Intelligent Laboratory Systems
volume
142
pages
95 - 106
publisher
Elsevier
external identifiers
  • wos:000351972500012
  • scopus:84922370254
ISSN
0169-7439
DOI
10.1016/j.chemolab.2015.01.014
language
English
LU publication?
yes
id
d5b5f134-8455-4b32-b754-4864da65a544 (old id 5280836)
date added to LUP
2015-04-24 10:34:09
date last changed
2017-01-01 05:26:01
@article{d5b5f134-8455-4b32-b754-4864da65a544,
  abstract     = {We present a set of spectrally and temporally resolved clinical fluorescence data-with two separate excitation wavelengths-that was recorded in vivo. We demonstrate that data in the spectral and temporal domains are in certain ways coupled and provide a method for integrated and effective parameterization of spectrally and temporally resolved fluorescence (i.e., time-resolved emission spectra). This parameterization is based on linear algebra, matrix formulation and system identification. We demonstrate how to empirically extract single exponentially decaying components and provide rectified emission spectra without prior knowledge. We investigate the potential for improved cancer diagnostics according to the reduced parameters along the various domains. In this case, in terms of cancer diagnostics, we were unable to identify any benefits of simultaneously measuring both the temporal and spectral properties of the observed fluorescence. However, we note that this may be explained by an important experimental bias present in many studies of optical cancer diagnostics, namely, that, in general, suspected lesions always differ visually from the neighboring healthy tissue. (C) 2015 Elsevier B.V. All rights reserved.},
  author       = {Brydegaard, Mikkel and Thompson, Alexander J. and Andersson-Engels, Stefan and Bendsöe, Niels and Svanberg, Katarina and Svanberg, Sune},
  issn         = {0169-7439},
  keyword      = {Biophotonics,Fluorescence lifetime,Optical spectroscopy,Dynamic,models,Population dynamics,Laser diagnostics},
  language     = {eng},
  pages        = {95--106},
  publisher    = {Elsevier},
  series       = {Chemometrics and Intelligent Laboratory Systems},
  title        = {Complete parameterization of temporally and spectrally resolved laser induced fluorescence data with applications in bio-photonics},
  url          = {http://dx.doi.org/10.1016/j.chemolab.2015.01.014},
  volume       = {142},
  year         = {2015},
}