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Bacterial Community Analysis of Drinking Water Biofilms in Southern Sweden

Luhrig, Katharina; Canbäck, Björn LU ; Paul, Catherine LU ; Johansson, Tomas LU ; Persson, Kenneth M LU and Rådström, Peter LU (2015) In Microbes and Environments 30(1). p.99-107
Abstract
Next-generation sequencing of the V1-V2 and V3 variable regions of the 16S rRNA gene generated a total of 674,116 reads that described six distinct bacterial biofilm communities from both water meters and pipes. A high degree of reproducibility was demonstrated for the experimental and analytical work-flow by analyzing the communities present in parallel water meters, the rare occurrence of biological replicates within a working drinking water distribution system. The communities observed in water meters from households that did not complain about their drinking water were defined by sequences representing Proteobacteria (82-87%), with 22-40% of all sequences being classified as Sphingomonadaceae. However, a water meter biofilm community... (More)
Next-generation sequencing of the V1-V2 and V3 variable regions of the 16S rRNA gene generated a total of 674,116 reads that described six distinct bacterial biofilm communities from both water meters and pipes. A high degree of reproducibility was demonstrated for the experimental and analytical work-flow by analyzing the communities present in parallel water meters, the rare occurrence of biological replicates within a working drinking water distribution system. The communities observed in water meters from households that did not complain about their drinking water were defined by sequences representing Proteobacteria (82-87%), with 22-40% of all sequences being classified as Sphingomonadaceae. However, a water meter biofilm community from a household with consumer reports of red water and flowing water containing elevated levels of iron and manganese had fewer sequences representing Proteobacteria (44%); only 0.6% of all sequences were classified as Sphingomonadaceae; and, in contrast to the other water meter communities, markedly more sequences represented Nitrospira and Pedomicrobium. The biofilm communities in pipes were distinct from those in water meters, and contained sequences that were identified as Mycobacterium, Nocardia, Desulfovibrio, and Sulfuricurvum. The approach employed in the present study resolved the bacterial diversity present in these biofilm communities as well as the differences that occurred in biofilms within a single distribution system, and suggests that next-generation sequencing of 16S rRNA amplicons can show changes in bacterial biofilm communities associated with different water qualities. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
drinking water, biofilm, next-generation sequencing, bacterial, communities, 16S rRNA pyrosequencing
in
Microbes and Environments
volume
30
issue
1
pages
99 - 107
publisher
Japanese Society of Microbial Ecology The Japanese Society of Soil Microbiology
external identifiers
  • wos:000351925200013
  • scopus:84925423309
ISSN
1342-6311
DOI
10.1264/jsme2.ME14123
language
English
LU publication?
yes
id
8e5995c0-ac2b-47f1-ade6-90ee2089f959 (old id 5281634)
date added to LUP
2015-04-24 08:45:29
date last changed
2017-11-12 03:04:41
@article{8e5995c0-ac2b-47f1-ade6-90ee2089f959,
  abstract     = {Next-generation sequencing of the V1-V2 and V3 variable regions of the 16S rRNA gene generated a total of 674,116 reads that described six distinct bacterial biofilm communities from both water meters and pipes. A high degree of reproducibility was demonstrated for the experimental and analytical work-flow by analyzing the communities present in parallel water meters, the rare occurrence of biological replicates within a working drinking water distribution system. The communities observed in water meters from households that did not complain about their drinking water were defined by sequences representing Proteobacteria (82-87%), with 22-40% of all sequences being classified as Sphingomonadaceae. However, a water meter biofilm community from a household with consumer reports of red water and flowing water containing elevated levels of iron and manganese had fewer sequences representing Proteobacteria (44%); only 0.6% of all sequences were classified as Sphingomonadaceae; and, in contrast to the other water meter communities, markedly more sequences represented Nitrospira and Pedomicrobium. The biofilm communities in pipes were distinct from those in water meters, and contained sequences that were identified as Mycobacterium, Nocardia, Desulfovibrio, and Sulfuricurvum. The approach employed in the present study resolved the bacterial diversity present in these biofilm communities as well as the differences that occurred in biofilms within a single distribution system, and suggests that next-generation sequencing of 16S rRNA amplicons can show changes in bacterial biofilm communities associated with different water qualities.},
  author       = {Luhrig, Katharina and Canbäck, Björn and Paul, Catherine and Johansson, Tomas and Persson, Kenneth M and Rådström, Peter},
  issn         = {1342-6311},
  keyword      = {drinking water,biofilm,next-generation sequencing,bacterial,communities,16S rRNA pyrosequencing},
  language     = {eng},
  number       = {1},
  pages        = {99--107},
  publisher    = {Japanese Society of Microbial Ecology The Japanese Society of Soil Microbiology},
  series       = {Microbes and Environments},
  title        = {Bacterial Community Analysis of Drinking Water Biofilms in Southern Sweden},
  url          = {http://dx.doi.org/10.1264/jsme2.ME14123},
  volume       = {30},
  year         = {2015},
}