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Increased magnitude of relaxation to oestrogen in aorta from oestrogen receptor beta knock-out mice

Nilsson, B O LU ; Ekblad, E LU ; Heine, T and Gustafsson, J-Å (2000) In Journal of Endocrinology 166(2). p.5-9
Abstract

Micromolar concentrations of the biologically active oestrogen 17beta-oestradiol reduce agonist-induced force in vascular preparations through an unidentified mechanism. The aim of the present study was to investigate the importance of oestrogen receptor beta (ERbeta) for oestrogen-induced vascular relaxation. 17beta-oestradiol was added to aortic rings from ERbeta knock-out (-/-) and wild-type (+/+) mice precontracted with noradrenaline. 17beta-oestradiol caused a concentration-dependent (1-100 microM) relaxation of aortic rings from both -/- and +/+ animals of both sexes. Rings from male and female -/- mice were more sensitive to 17beta-oestradiol than those from +/+ mice. Medial thickness, determined by computerized image analysis,... (More)

Micromolar concentrations of the biologically active oestrogen 17beta-oestradiol reduce agonist-induced force in vascular preparations through an unidentified mechanism. The aim of the present study was to investigate the importance of oestrogen receptor beta (ERbeta) for oestrogen-induced vascular relaxation. 17beta-oestradiol was added to aortic rings from ERbeta knock-out (-/-) and wild-type (+/+) mice precontracted with noradrenaline. 17beta-oestradiol caused a concentration-dependent (1-100 microM) relaxation of aortic rings from both -/- and +/+ animals of both sexes. Rings from male and female -/- mice were more sensitive to 17beta-oestradiol than those from +/+ mice. Medial thickness, determined by computerized image analysis, was similar in rings from -/- and +/+ animals. Endothelium, as determined by immuno-cytochemistry, was present in -/- and +/+ aorta. Maximal noradrenaline evoked force and sensitivity to noradrenaline were similar in both groups. In summary ERbeta modulates vascular relaxation to microM concentrations of oestrogen; lack of ERbeta renders the vascular wall supersensitive to 17beta-oestradiol. Lack of ERbeta caused no change in vascular wall morphology suggesting that this ER subtype is not involved in vascular structure development.

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keywords
Animals, Aorta, Body Weight, Dose-Response Relationship, Drug, Endothelium, Vascular, Estradiol, Estrogen Receptor beta, Female, Image Processing, Computer-Assisted, Immunohistochemistry, In Vitro Techniques, Male, Mice, Mice, Knockout, Microscopy, Fluorescence, Muscle, Smooth, Vascular, Norepinephrine, Receptors, Estrogen, Vasoconstriction
in
Journal of Endocrinology
volume
166
issue
2
pages
5 - 9
publisher
Society for Endocrinology
external identifiers
  • Scopus:0033838852
ISSN
0022-0795
language
English
LU publication?
yes
id
52cd1147-bee6-4b34-aa9e-15014b586e48
date added to LUP
2016-06-17 16:54:12
date last changed
2017-01-01 08:28:24
@article{52cd1147-bee6-4b34-aa9e-15014b586e48,
  abstract     = {<p>Micromolar concentrations of the biologically active oestrogen 17beta-oestradiol reduce agonist-induced force in vascular preparations through an unidentified mechanism. The aim of the present study was to investigate the importance of oestrogen receptor beta (ERbeta) for oestrogen-induced vascular relaxation. 17beta-oestradiol was added to aortic rings from ERbeta knock-out (-/-) and wild-type (+/+) mice precontracted with noradrenaline. 17beta-oestradiol caused a concentration-dependent (1-100 microM) relaxation of aortic rings from both -/- and +/+ animals of both sexes. Rings from male and female -/- mice were more sensitive to 17beta-oestradiol than those from +/+ mice. Medial thickness, determined by computerized image analysis, was similar in rings from -/- and +/+ animals. Endothelium, as determined by immuno-cytochemistry, was present in -/- and +/+ aorta. Maximal noradrenaline evoked force and sensitivity to noradrenaline were similar in both groups. In summary ERbeta modulates vascular relaxation to microM concentrations of oestrogen; lack of ERbeta renders the vascular wall supersensitive to 17beta-oestradiol. Lack of ERbeta caused no change in vascular wall morphology suggesting that this ER subtype is not involved in vascular structure development.</p>},
  author       = {Nilsson, B O and Ekblad, E and Heine, T and Gustafsson, J-Å},
  issn         = {0022-0795},
  keyword      = {Animals,Aorta,Body Weight,Dose-Response Relationship, Drug,Endothelium, Vascular,Estradiol,Estrogen Receptor beta,Female,Image Processing, Computer-Assisted,Immunohistochemistry,In Vitro Techniques,Male,Mice,Mice, Knockout,Microscopy, Fluorescence,Muscle, Smooth, Vascular,Norepinephrine,Receptors, Estrogen,Vasoconstriction},
  language     = {eng},
  number       = {2},
  pages        = {5--9},
  publisher    = {Society for Endocrinology},
  series       = {Journal of Endocrinology},
  title        = {Increased magnitude of relaxation to oestrogen in aorta from oestrogen receptor beta knock-out mice},
  volume       = {166},
  year         = {2000},
}