Vascular function in the cadaver up to six hours after cardiac arrest
(1999) In The Journal of Heart and Lung Transplantation 18(6). p.582-586- Abstract
- BACKGROUND: The aim of the study was to evaluate how well vascular function is retained in a cadaver kept in a room with a temperature of 21 degrees C. METHODS: The aorta and pulmonary artery of rats were investigated in organ baths as fresh controls and after 1, 2, 3, or 6 hours' storage in the cadaver. Six-hour-old cadaver aortas were transplanted and investigated after 24 hours and 60 days. RESULTS: After 3 hours' storage there was no significant decrease in smooth muscle contractile function in either aorta or pulmonary artery. After 6 hours' storage both the aorta and the pulmonary artery demonstrated a significant decrease in smooth muscle contractile function, 30% (p < 0.05) and 44% (p < 0.001), respectively, compared to fresh... (More)
- BACKGROUND: The aim of the study was to evaluate how well vascular function is retained in a cadaver kept in a room with a temperature of 21 degrees C. METHODS: The aorta and pulmonary artery of rats were investigated in organ baths as fresh controls and after 1, 2, 3, or 6 hours' storage in the cadaver. Six-hour-old cadaver aortas were transplanted and investigated after 24 hours and 60 days. RESULTS: After 3 hours' storage there was no significant decrease in smooth muscle contractile function in either aorta or pulmonary artery. After 6 hours' storage both the aorta and the pulmonary artery demonstrated a significant decrease in smooth muscle contractile function, 30% (p < 0.05) and 44% (p < 0.001), respectively, compared to fresh controls. Storing the aorta for 2 hours and the pulmonary artery for 6 hours caused no significant decrease in endothelium-dependent relaxing function. In aorta segments investigated after 3 and 6 hours there was a significant decrease in endothelium-dependent relaxation, 12% (p < 0.05) and 29% (p < 0.001), respectively. Six-hour-old cadaver aortas transplanted and investigated after 24 hours or 60 days demonstrated no significant changes in endothelium-dependent relaxation and smooth muscle function compared to fresh controls. CONCLUSION: The pulmonary artery can tolerate 3 hours of warm ischemia in the nonheart-beating cadaver without loss of endothelium-dependent relaxation and smooth muscle function. The dysfunction seen in 6-hour-old cadaver aortas was normalized after transplantation and 24 hours of reperfusion. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1115878
- author
- Bolys, Ramunas ; Ingemansson, Richard LU ; Sjöberg, Trygve LU and Steen, Stig LU
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The Journal of Heart and Lung Transplantation
- volume
- 18
- issue
- 6
- pages
- 582 - 586
- publisher
- Elsevier
- external identifiers
-
- pmid:10395356
- scopus:0033063689
- ISSN
- 1557-3117
- DOI
- 10.1016/S1053-2498(98)00075-8
- language
- English
- LU publication?
- yes
- id
- 52dada87-452e-4c6b-bc18-3e9134fecac7 (old id 1115878)
- date added to LUP
- 2016-04-01 12:17:12
- date last changed
- 2022-01-27 01:33:59
@article{52dada87-452e-4c6b-bc18-3e9134fecac7, abstract = {{BACKGROUND: The aim of the study was to evaluate how well vascular function is retained in a cadaver kept in a room with a temperature of 21 degrees C. METHODS: The aorta and pulmonary artery of rats were investigated in organ baths as fresh controls and after 1, 2, 3, or 6 hours' storage in the cadaver. Six-hour-old cadaver aortas were transplanted and investigated after 24 hours and 60 days. RESULTS: After 3 hours' storage there was no significant decrease in smooth muscle contractile function in either aorta or pulmonary artery. After 6 hours' storage both the aorta and the pulmonary artery demonstrated a significant decrease in smooth muscle contractile function, 30% (p < 0.05) and 44% (p < 0.001), respectively, compared to fresh controls. Storing the aorta for 2 hours and the pulmonary artery for 6 hours caused no significant decrease in endothelium-dependent relaxing function. In aorta segments investigated after 3 and 6 hours there was a significant decrease in endothelium-dependent relaxation, 12% (p < 0.05) and 29% (p < 0.001), respectively. Six-hour-old cadaver aortas transplanted and investigated after 24 hours or 60 days demonstrated no significant changes in endothelium-dependent relaxation and smooth muscle function compared to fresh controls. CONCLUSION: The pulmonary artery can tolerate 3 hours of warm ischemia in the nonheart-beating cadaver without loss of endothelium-dependent relaxation and smooth muscle function. The dysfunction seen in 6-hour-old cadaver aortas was normalized after transplantation and 24 hours of reperfusion.}}, author = {{Bolys, Ramunas and Ingemansson, Richard and Sjöberg, Trygve and Steen, Stig}}, issn = {{1557-3117}}, language = {{eng}}, number = {{6}}, pages = {{582--586}}, publisher = {{Elsevier}}, series = {{The Journal of Heart and Lung Transplantation}}, title = {{Vascular function in the cadaver up to six hours after cardiac arrest}}, url = {{http://dx.doi.org/10.1016/S1053-2498(98)00075-8}}, doi = {{10.1016/S1053-2498(98)00075-8}}, volume = {{18}}, year = {{1999}}, }