Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets
(1989) In Drug and Alcohol Dependence 24(2). p.169-174- Abstract
- The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1104753
- author
- Simonsson, Per LU ; Ferencz, Istvan LU and Alling, Christer LU
- organization
- publishing date
- 1989
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ethanol, phospholipase C, serotonin, phosphatidic acid, platelets
- in
- Drug and Alcohol Dependence
- volume
- 24
- issue
- 2
- pages
- 169 - 174
- publisher
- Elsevier
- external identifiers
-
- pmid:2791892
- scopus:0024452304
- ISSN
- 1879-0046
- DOI
- 10.1016/0376-8716(89)90081-1
- language
- English
- LU publication?
- yes
- id
- 53150406-c283-48b2-9fda-a23a58d37a44 (old id 1104753)
- date added to LUP
- 2016-04-01 12:31:16
- date last changed
- 2021-01-03 07:17:53
@article{53150406-c283-48b2-9fda-a23a58d37a44, abstract = {{The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.}}, author = {{Simonsson, Per and Ferencz, Istvan and Alling, Christer}}, issn = {{1879-0046}}, keywords = {{ethanol; phospholipase C; serotonin; phosphatidic acid; platelets}}, language = {{eng}}, number = {{2}}, pages = {{169--174}}, publisher = {{Elsevier}}, series = {{Drug and Alcohol Dependence}}, title = {{Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets}}, url = {{http://dx.doi.org/10.1016/0376-8716(89)90081-1}}, doi = {{10.1016/0376-8716(89)90081-1}}, volume = {{24}}, year = {{1989}}, }