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Embedded 3D Printing in Self-Healing Annealable Composites for Precise Patterning of Functionally Mature Human Neural Constructs

Kajtez, Janko LU orcid ; Wesseler, Milan Finn ; Birtele, Marcella LU orcid ; Khorasgani, Farinaz Riyahi ; Rylander Ottosson, Daniella LU ; Heiskanen, Arto LU ; Kamperman, Tom ; Leijten, Jeroen ; Martínez-Serrano, Alberto and Larsen, Niels B , et al. (2022) In Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Abstract

Human in vitro models of neural tissue with tunable microenvironment and defined spatial arrangement are needed to facilitate studies of brain development and disease. Towards this end, embedded printing inside granular gels holds great promise as it allows precise patterning of extremely soft tissue constructs. However, granular printing support formulations are restricted to only a handful of materials. Therefore, there has been a need for novel materials that take advantage of versatile biomimicry of bulk hydrogels while providing high-fidelity support for embedded printing akin to granular gels. To address this need, Authors present a modular platform for bioengineering of neuronal networks via direct embedded 3D printing of human... (More)

Human in vitro models of neural tissue with tunable microenvironment and defined spatial arrangement are needed to facilitate studies of brain development and disease. Towards this end, embedded printing inside granular gels holds great promise as it allows precise patterning of extremely soft tissue constructs. However, granular printing support formulations are restricted to only a handful of materials. Therefore, there has been a need for novel materials that take advantage of versatile biomimicry of bulk hydrogels while providing high-fidelity support for embedded printing akin to granular gels. To address this need, Authors present a modular platform for bioengineering of neuronal networks via direct embedded 3D printing of human stem cells inside Self-Healing Annealable Particle-Extracellular matrix (SHAPE) composites. SHAPE composites consist of soft microgels immersed in viscous extracellular-matrix solution to enable precise and programmable patterning of human stem cells and consequent generation mature subtype-specific neurons that extend projections into the volume of the annealed support. The developed approach further allows multi-ink deposition, live spatial and temporal monitoring of oxygen levels, as well as creation of vascular-like channels. Due to its modularity and versatility, SHAPE biomanufacturing toolbox has potential to be used in applications beyond functional modeling of mechanically sensitive neural constructs.

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organization
publishing date
type
Contribution to journal
publication status
epub
subject
in
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
article number
e2201392
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:85131955321
  • pmid:35712780
ISSN
2198-3844
DOI
10.1002/advs.202201392
project
Development of Biomaterial-based Delivery Systems for Parkinson’s Disease- An Integrated Pan-European Approach
language
English
LU publication?
yes
additional info
© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.
id
531e830e-8b2f-4bad-8d1c-7620e54ad790
date added to LUP
2022-08-02 16:15:42
date last changed
2022-10-01 07:15:26
@article{531e830e-8b2f-4bad-8d1c-7620e54ad790,
  abstract     = {{<p>Human in vitro models of neural tissue with tunable microenvironment and defined spatial arrangement are needed to facilitate studies of brain development and disease. Towards this end, embedded printing inside granular gels holds great promise as it allows precise patterning of extremely soft tissue constructs. However, granular printing support formulations are restricted to only a handful of materials. Therefore, there has been a need for novel materials that take advantage of versatile biomimicry of bulk hydrogels while providing high-fidelity support for embedded printing akin to granular gels. To address this need, Authors present a modular platform for bioengineering of neuronal networks via direct embedded 3D printing of human stem cells inside Self-Healing Annealable Particle-Extracellular matrix (SHAPE) composites. SHAPE composites consist of soft microgels immersed in viscous extracellular-matrix solution to enable precise and programmable patterning of human stem cells and consequent generation mature subtype-specific neurons that extend projections into the volume of the annealed support. The developed approach further allows multi-ink deposition, live spatial and temporal monitoring of oxygen levels, as well as creation of vascular-like channels. Due to its modularity and versatility, SHAPE biomanufacturing toolbox has potential to be used in applications beyond functional modeling of mechanically sensitive neural constructs.</p>}},
  author       = {{Kajtez, Janko and Wesseler, Milan Finn and Birtele, Marcella and Khorasgani, Farinaz Riyahi and Rylander Ottosson, Daniella and Heiskanen, Arto and Kamperman, Tom and Leijten, Jeroen and Martínez-Serrano, Alberto and Larsen, Niels B and Angelini, Thomas E and Parmar, Malin and Lind, Johan U and Emnéus, Jenny}},
  issn         = {{2198-3844}},
  language     = {{eng}},
  month        = {{06}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Advanced science (Weinheim, Baden-Wurttemberg, Germany)}},
  title        = {{Embedded 3D Printing in Self-Healing Annealable Composites for Precise Patterning of Functionally Mature Human Neural Constructs}},
  url          = {{http://dx.doi.org/10.1002/advs.202201392}},
  doi          = {{10.1002/advs.202201392}},
  year         = {{2022}},
}