T Follicular Helper, but Not Th1, Cell Differentiation in the Absence of Conventional Dendritic Cells.
(2015) In Journal of Immunology 194(11). p.5187-5199- Abstract
- Development of long-lived humoral immunity is dependent on CXCR5-expressing T follicular helper (Tfh) cells, which develop concomitantly to effector Th cells that support cellular immunity. Conventional dendritic cells (cDCs) are critical APCs for initial priming of naive CD4(+) T cells but, importantly, also provide accessory signals that govern effector Th cell commitment. To define the accessory role of cDCs during the concurrent development of Tfh and effector Th1 cells, we performed high-dose Ag immunization in conjunction with the Th1-biased adjuvant polyinosinic:polycytidylic acid (pI:C). In the absence of cDCs, pI:C failed to induce Th1 cell commitment and IgG2c production. However, cDC depletion did not impair Tfh cell... (More)
- Development of long-lived humoral immunity is dependent on CXCR5-expressing T follicular helper (Tfh) cells, which develop concomitantly to effector Th cells that support cellular immunity. Conventional dendritic cells (cDCs) are critical APCs for initial priming of naive CD4(+) T cells but, importantly, also provide accessory signals that govern effector Th cell commitment. To define the accessory role of cDCs during the concurrent development of Tfh and effector Th1 cells, we performed high-dose Ag immunization in conjunction with the Th1-biased adjuvant polyinosinic:polycytidylic acid (pI:C). In the absence of cDCs, pI:C failed to induce Th1 cell commitment and IgG2c production. However, cDC depletion did not impair Tfh cell differentiation or germinal center formation, and long-lived IgG1 responses of unaltered affinity developed in mice lacking cDCs at the time point for immunization. Thus, cDCs are required for the pI:C-driven Th1 cell fate commitment but have no crucial accessory function in relation to Tfh cell differentiation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5337734
- author
- Dahlgren, Madelene
LU
; Gustafsson-Hedberg, Tobias ; Livingston, Megan ; Cucak, Helena LU ; Alsén, Samuel LU ; Yrlid, Ulf and Johansson Lindbom, Bengt LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Immunology
- volume
- 194
- issue
- 11
- pages
- 5187 - 5199
- publisher
- American Association of Immunologists
- external identifiers
-
- pmid:25917099
- wos:000354905300020
- scopus:84929646119
- ISSN
- 1550-6606
- DOI
- 10.4049/jimmunol.1401938
- language
- English
- LU publication?
- yes
- id
- 21dbb07d-d2d6-43a1-8b04-a7c23b656a23 (old id 5337734)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25917099?dopt=Abstract
- date added to LUP
- 2016-04-01 10:20:59
- date last changed
- 2024-02-05 02:52:35
@article{21dbb07d-d2d6-43a1-8b04-a7c23b656a23, abstract = {{Development of long-lived humoral immunity is dependent on CXCR5-expressing T follicular helper (Tfh) cells, which develop concomitantly to effector Th cells that support cellular immunity. Conventional dendritic cells (cDCs) are critical APCs for initial priming of naive CD4(+) T cells but, importantly, also provide accessory signals that govern effector Th cell commitment. To define the accessory role of cDCs during the concurrent development of Tfh and effector Th1 cells, we performed high-dose Ag immunization in conjunction with the Th1-biased adjuvant polyinosinic:polycytidylic acid (pI:C). In the absence of cDCs, pI:C failed to induce Th1 cell commitment and IgG2c production. However, cDC depletion did not impair Tfh cell differentiation or germinal center formation, and long-lived IgG1 responses of unaltered affinity developed in mice lacking cDCs at the time point for immunization. Thus, cDCs are required for the pI:C-driven Th1 cell fate commitment but have no crucial accessory function in relation to Tfh cell differentiation.}}, author = {{Dahlgren, Madelene and Gustafsson-Hedberg, Tobias and Livingston, Megan and Cucak, Helena and Alsén, Samuel and Yrlid, Ulf and Johansson Lindbom, Bengt}}, issn = {{1550-6606}}, language = {{eng}}, number = {{11}}, pages = {{5187--5199}}, publisher = {{American Association of Immunologists}}, series = {{Journal of Immunology}}, title = {{T Follicular Helper, but Not Th1, Cell Differentiation in the Absence of Conventional Dendritic Cells.}}, url = {{http://dx.doi.org/10.4049/jimmunol.1401938}}, doi = {{10.4049/jimmunol.1401938}}, volume = {{194}}, year = {{2015}}, }