Advanced

T Follicular Helper, but Not Th1, Cell Differentiation in the Absence of Conventional Dendritic Cells.

Dahlgren, Madelene LU ; Gustafsson-Hedberg, Tobias; Livingston, Megan; Cucak, Helena LU ; Alsén, Samuel LU ; Yrlid, Ulf and Johansson Lindbom, Bengt LU (2015) In Journal of Immunology 194(11). p.5187-5199
Abstract
Development of long-lived humoral immunity is dependent on CXCR5-expressing T follicular helper (Tfh) cells, which develop concomitantly to effector Th cells that support cellular immunity. Conventional dendritic cells (cDCs) are critical APCs for initial priming of naive CD4(+) T cells but, importantly, also provide accessory signals that govern effector Th cell commitment. To define the accessory role of cDCs during the concurrent development of Tfh and effector Th1 cells, we performed high-dose Ag immunization in conjunction with the Th1-biased adjuvant polyinosinic:polycytidylic acid (pI:C). In the absence of cDCs, pI:C failed to induce Th1 cell commitment and IgG2c production. However, cDC depletion did not impair Tfh cell... (More)
Development of long-lived humoral immunity is dependent on CXCR5-expressing T follicular helper (Tfh) cells, which develop concomitantly to effector Th cells that support cellular immunity. Conventional dendritic cells (cDCs) are critical APCs for initial priming of naive CD4(+) T cells but, importantly, also provide accessory signals that govern effector Th cell commitment. To define the accessory role of cDCs during the concurrent development of Tfh and effector Th1 cells, we performed high-dose Ag immunization in conjunction with the Th1-biased adjuvant polyinosinic:polycytidylic acid (pI:C). In the absence of cDCs, pI:C failed to induce Th1 cell commitment and IgG2c production. However, cDC depletion did not impair Tfh cell differentiation or germinal center formation, and long-lived IgG1 responses of unaltered affinity developed in mice lacking cDCs at the time point for immunization. Thus, cDCs are required for the pI:C-driven Th1 cell fate commitment but have no crucial accessory function in relation to Tfh cell differentiation. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Immunology
volume
194
issue
11
pages
5187 - 5199
publisher
American Association of Immunologists
external identifiers
  • pmid:25917099
  • wos:000354905300020
  • scopus:84929646119
ISSN
1550-6606
DOI
10.4049/jimmunol.1401938
language
English
LU publication?
yes
id
21dbb07d-d2d6-43a1-8b04-a7c23b656a23 (old id 5337734)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25917099?dopt=Abstract
date added to LUP
2015-05-01 22:31:20
date last changed
2017-09-24 03:10:53
@article{21dbb07d-d2d6-43a1-8b04-a7c23b656a23,
  abstract     = {Development of long-lived humoral immunity is dependent on CXCR5-expressing T follicular helper (Tfh) cells, which develop concomitantly to effector Th cells that support cellular immunity. Conventional dendritic cells (cDCs) are critical APCs for initial priming of naive CD4(+) T cells but, importantly, also provide accessory signals that govern effector Th cell commitment. To define the accessory role of cDCs during the concurrent development of Tfh and effector Th1 cells, we performed high-dose Ag immunization in conjunction with the Th1-biased adjuvant polyinosinic:polycytidylic acid (pI:C). In the absence of cDCs, pI:C failed to induce Th1 cell commitment and IgG2c production. However, cDC depletion did not impair Tfh cell differentiation or germinal center formation, and long-lived IgG1 responses of unaltered affinity developed in mice lacking cDCs at the time point for immunization. Thus, cDCs are required for the pI:C-driven Th1 cell fate commitment but have no crucial accessory function in relation to Tfh cell differentiation.},
  author       = {Dahlgren, Madelene and Gustafsson-Hedberg, Tobias and Livingston, Megan and Cucak, Helena and Alsén, Samuel and Yrlid, Ulf and Johansson Lindbom, Bengt},
  issn         = {1550-6606},
  language     = {eng},
  number       = {11},
  pages        = {5187--5199},
  publisher    = {American Association of Immunologists},
  series       = {Journal of Immunology},
  title        = {T Follicular Helper, but Not Th1, Cell Differentiation in the Absence of Conventional Dendritic Cells.},
  url          = {http://dx.doi.org/10.4049/jimmunol.1401938},
  volume       = {194},
  year         = {2015},
}