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Acetylation of intragenic histones on HPV16 correlates with enhanced HPV16 gene expression.

Johansson, Cecilia LU ; Jamal Fattah, Tavan; Yu, Haoran LU ; Nygren, Jakob; Mossberg, Anki LU and Schwartz, Stefan LU (2015) In Virology 482. p.244-259
Abstract
We report that many histone modifications are unevenly distributed over the HPV16 genome in cervical cancer cells as well as in HPV16-immortalized keratinocytes. For example, H3K36me3 and H3K9Ac that are common in highly expressed cellular genes and over exons, were more common in the early than in the late region of the HPV16 genome. In contrast, H3K9me3, H4K20me3, H2BK5me1 and H4K16Ac were more frequent in the HPV16 late region. Furthermore, a region encompassing the HPV16 early polyadenylation signal pAE displayed high levels of histone H3 acetylation. Histone deacetylase (HDAC) inhibitors caused a 2- to 8-fold induction of HPV16 early and late mRNAs in cervical cancer cells and in immortalized keratinocytes, while at the same time... (More)
We report that many histone modifications are unevenly distributed over the HPV16 genome in cervical cancer cells as well as in HPV16-immortalized keratinocytes. For example, H3K36me3 and H3K9Ac that are common in highly expressed cellular genes and over exons, were more common in the early than in the late region of the HPV16 genome. In contrast, H3K9me3, H4K20me3, H2BK5me1 and H4K16Ac were more frequent in the HPV16 late region. Furthermore, a region encompassing the HPV16 early polyadenylation signal pAE displayed high levels of histone H3 acetylation. Histone deacetylase (HDAC) inhibitors caused a 2- to 8-fold induction of HPV16 early and late mRNAs in cervical cancer cells and in immortalized keratinocytes, while at the same time increasing the levels of acetylated histones in the cells and on the HPV16 genome specifically. We concluded that increased histone acetylation on the HPV16 genome correlates with increased HPV16 gene expression. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Virology
volume
482
pages
244 - 259
publisher
Elsevier
external identifiers
  • pmid:25900886
  • wos:000356401900030
  • scopus:84927738009
ISSN
1096-0341
DOI
10.1016/j.virol.2015.02.053
language
English
LU publication?
yes
id
14b7455d-1d20-415c-ab90-211985ce8fed (old id 5340973)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25900886?dopt=Abstract
date added to LUP
2015-05-02 19:23:33
date last changed
2017-02-26 03:19:43
@article{14b7455d-1d20-415c-ab90-211985ce8fed,
  abstract     = {We report that many histone modifications are unevenly distributed over the HPV16 genome in cervical cancer cells as well as in HPV16-immortalized keratinocytes. For example, H3K36me3 and H3K9Ac that are common in highly expressed cellular genes and over exons, were more common in the early than in the late region of the HPV16 genome. In contrast, H3K9me3, H4K20me3, H2BK5me1 and H4K16Ac were more frequent in the HPV16 late region. Furthermore, a region encompassing the HPV16 early polyadenylation signal pAE displayed high levels of histone H3 acetylation. Histone deacetylase (HDAC) inhibitors caused a 2- to 8-fold induction of HPV16 early and late mRNAs in cervical cancer cells and in immortalized keratinocytes, while at the same time increasing the levels of acetylated histones in the cells and on the HPV16 genome specifically. We concluded that increased histone acetylation on the HPV16 genome correlates with increased HPV16 gene expression.},
  author       = {Johansson, Cecilia and Jamal Fattah, Tavan and Yu, Haoran and Nygren, Jakob and Mossberg, Anki and Schwartz, Stefan},
  issn         = {1096-0341},
  language     = {eng},
  pages        = {244--259},
  publisher    = {Elsevier},
  series       = {Virology},
  title        = {Acetylation of intragenic histones on HPV16 correlates with enhanced HPV16 gene expression.},
  url          = {http://dx.doi.org/10.1016/j.virol.2015.02.053},
  volume       = {482},
  year         = {2015},
}