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Controlled exposure to diesel exhaust and traffic noise - Effects on oxidative stress and activation in mononuclear blood cells.

Hemmingsen, Jette Gjerke; Møller, Peter; Jantzen, Kim; Jönsson, Bo A LU ; Albin, Maria LU ; Wierzbicka, Aneta; Gudmundsson, Anders; Loft, Steffen and Rissler, Jenny (2015) In Mutation Research 775. p.66-71
Abstract
Particulate air pollution increases risk of cancer and cardiopulmonary disease, partly through oxidative stress. Traffic-related noise increases risk of cardiovascular disease and may cause oxidative stress. In this controlled random sequence study, 18 healthy subjects were exposed for 3h to diesel exhaust (DE) at 276μg/m(3) from a passenger car or filtered air, with co-exposure to traffic noise at 48 or 75dB(A). Gene expression markers of inflammation, (interleukin-8 and tumor necrosis factor), oxidative stress (heme oxygenase (decycling-1)) and DNA repair (8-oxoguanine DNA glycosylase (OGG1)) were unaltered in peripheral blood mononuclear cells (PBMCs). No significant differences in DNA damage levels, measured by the comet assay, were... (More)
Particulate air pollution increases risk of cancer and cardiopulmonary disease, partly through oxidative stress. Traffic-related noise increases risk of cardiovascular disease and may cause oxidative stress. In this controlled random sequence study, 18 healthy subjects were exposed for 3h to diesel exhaust (DE) at 276μg/m(3) from a passenger car or filtered air, with co-exposure to traffic noise at 48 or 75dB(A). Gene expression markers of inflammation, (interleukin-8 and tumor necrosis factor), oxidative stress (heme oxygenase (decycling-1)) and DNA repair (8-oxoguanine DNA glycosylase (OGG1)) were unaltered in peripheral blood mononuclear cells (PBMCs). No significant differences in DNA damage levels, measured by the comet assay, were observed after DE exposure, whereas exposure to high noise levels was associated with significantly increased levels of hOGG1-sensitive sites in PBMCs. Urinary levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine were unaltered. In auxiliary ex vivo experiments whole blood was incubated with particles from the exposure chamber for 3h without effects on DNA damage in PBMCs or intracellular reactive oxygen species production and expression of CD11b and CD62L adhesion molecules in leukocyte subtypes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Mutation Research
volume
775
pages
66 - 71
publisher
Elsevier
external identifiers
  • pmid:25898780
  • wos:000354584900010
  • scopus:84928580978
ISSN
1873-135X
DOI
10.1016/j.mrfmmm.2015.03.009
language
English
LU publication?
yes
id
8855691e-237c-44a5-a736-36c1a80442d7 (old id 5341032)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25898780?dopt=Abstract
date added to LUP
2015-05-04 10:13:42
date last changed
2017-08-06 03:15:46
@article{8855691e-237c-44a5-a736-36c1a80442d7,
  abstract     = {Particulate air pollution increases risk of cancer and cardiopulmonary disease, partly through oxidative stress. Traffic-related noise increases risk of cardiovascular disease and may cause oxidative stress. In this controlled random sequence study, 18 healthy subjects were exposed for 3h to diesel exhaust (DE) at 276μg/m(3) from a passenger car or filtered air, with co-exposure to traffic noise at 48 or 75dB(A). Gene expression markers of inflammation, (interleukin-8 and tumor necrosis factor), oxidative stress (heme oxygenase (decycling-1)) and DNA repair (8-oxoguanine DNA glycosylase (OGG1)) were unaltered in peripheral blood mononuclear cells (PBMCs). No significant differences in DNA damage levels, measured by the comet assay, were observed after DE exposure, whereas exposure to high noise levels was associated with significantly increased levels of hOGG1-sensitive sites in PBMCs. Urinary levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine were unaltered. In auxiliary ex vivo experiments whole blood was incubated with particles from the exposure chamber for 3h without effects on DNA damage in PBMCs or intracellular reactive oxygen species production and expression of CD11b and CD62L adhesion molecules in leukocyte subtypes.},
  author       = {Hemmingsen, Jette Gjerke and Møller, Peter and Jantzen, Kim and Jönsson, Bo A and Albin, Maria and Wierzbicka, Aneta and Gudmundsson, Anders and Loft, Steffen and Rissler, Jenny},
  issn         = {1873-135X},
  language     = {eng},
  pages        = {66--71},
  publisher    = {Elsevier},
  series       = {Mutation Research},
  title        = {Controlled exposure to diesel exhaust and traffic noise - Effects on oxidative stress and activation in mononuclear blood cells.},
  url          = {http://dx.doi.org/10.1016/j.mrfmmm.2015.03.009},
  volume       = {775},
  year         = {2015},
}