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Heterogeneous nuclear ribonucleoprotein C proteins interact with the human papillomavirus type 16 (HPV16) early 3'-untranslated region and alleviate suppression of HPV16 late L1 mRNA splicing.

Dhanjal, Soniya LU ; Kajitani, Naoko LU ; Glahder, Jacob LU ; Mossberg, Anki LU ; Johansson, Cecilia LU and Schwartz, Stefan LU (2015) In Journal of Biological Chemistry 290(21). p.13354-13371
Abstract
In order to identify cellular factors that regulate human papillomavirus type 16 (HPV16) gene expression, cervical cancer cells permissive for HPV16 late gene expression were identified and characterized. These cells either contained a novel spliced variant of the L1 mRNAs that bypassed the suppressed HPV16 late, 5'-splice site SD3632, produced elevated levels of RNA-binding proteins SRSF1 (ASF/SF2), SRSF9 (SRp30c) and HuR that are known to regulate HPV16 late gene expression, or were shown by a gene expression array analysis to overexpress the RALYL RNA-binding protein of the heterogeneous nuclear ribonucleoprotein C (hnRNP C)-family. Overexpression of RALYL or hnRNP C1 induced HPV16 late gene expression from HPV16 subgenomic plasmids and... (More)
In order to identify cellular factors that regulate human papillomavirus type 16 (HPV16) gene expression, cervical cancer cells permissive for HPV16 late gene expression were identified and characterized. These cells either contained a novel spliced variant of the L1 mRNAs that bypassed the suppressed HPV16 late, 5'-splice site SD3632, produced elevated levels of RNA-binding proteins SRSF1 (ASF/SF2), SRSF9 (SRp30c) and HuR that are known to regulate HPV16 late gene expression, or were shown by a gene expression array analysis to overexpress the RALYL RNA-binding protein of the heterogeneous nuclear ribonucleoprotein C (hnRNP C)-family. Overexpression of RALYL or hnRNP C1 induced HPV16 late gene expression from HPV16 subgenomic plasmids and from episomal forms of the full-length HPV16 genome. This induction was dependent on the HPV16 early untranslated region. Binding of hnRNP C1 to the HPV16 early, untranslated region activated HPV16 late 5'-splice site SD3632 and resulted in production of HPV16 L1 mRNAs. Our results suggested that hnRNP C1 controls HPV16 late gene expression. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
290
issue
21
pages
13354 - 13371
publisher
ASBMB
external identifiers
  • pmid:25878250
  • wos:000354975700033
  • scopus:84929993904
ISSN
1083-351X
DOI
10.1074/jbc.M115.638098
language
English
LU publication?
yes
id
e0f86d67-5ba0-473b-b3a8-028ebea769c7 (old id 5341761)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25878250?dopt=Abstract
date added to LUP
2015-05-05 18:19:34
date last changed
2017-09-17 03:58:36
@article{e0f86d67-5ba0-473b-b3a8-028ebea769c7,
  abstract     = {In order to identify cellular factors that regulate human papillomavirus type 16 (HPV16) gene expression, cervical cancer cells permissive for HPV16 late gene expression were identified and characterized. These cells either contained a novel spliced variant of the L1 mRNAs that bypassed the suppressed HPV16 late, 5'-splice site SD3632, produced elevated levels of RNA-binding proteins SRSF1 (ASF/SF2), SRSF9 (SRp30c) and HuR that are known to regulate HPV16 late gene expression, or were shown by a gene expression array analysis to overexpress the RALYL RNA-binding protein of the heterogeneous nuclear ribonucleoprotein C (hnRNP C)-family. Overexpression of RALYL or hnRNP C1 induced HPV16 late gene expression from HPV16 subgenomic plasmids and from episomal forms of the full-length HPV16 genome. This induction was dependent on the HPV16 early untranslated region. Binding of hnRNP C1 to the HPV16 early, untranslated region activated HPV16 late 5'-splice site SD3632 and resulted in production of HPV16 L1 mRNAs. Our results suggested that hnRNP C1 controls HPV16 late gene expression.},
  author       = {Dhanjal, Soniya and Kajitani, Naoko and Glahder, Jacob and Mossberg, Anki and Johansson, Cecilia and Schwartz, Stefan},
  issn         = {1083-351X},
  language     = {eng},
  number       = {21},
  pages        = {13354--13371},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Heterogeneous nuclear ribonucleoprotein C proteins interact with the human papillomavirus type 16 (HPV16) early 3'-untranslated region and alleviate suppression of HPV16 late L1 mRNA splicing.},
  url          = {http://dx.doi.org/10.1074/jbc.M115.638098},
  volume       = {290},
  year         = {2015},
}