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Label-free protein quantification in freshly ejaculated versus post-mating spermatophores of the noble crayfish Astacus astacus.

Niksirat, Hamid; James, Peter LU ; Andersson, Liselotte LU ; Kouba, Antonín and Kozák, Pavel (2015) In Journal of Proteomics 123. p.70-77
Abstract
Crayfish spermatophores are deposited on the body surface of the female during mating and remain there for a period of time before fertilization ensues. Post-mating changes in protein expression level in the noble crayfish Astacus astacus spermatophore were quantified. In-gel digestion and high resolution mass spectrometry were used for label-free protein quantification. One hundred twelve proteins were identified in the spermatophore of noble crayfish. After 7days of storage on the body of the female, 6 proteins were identified in the post-mating spermatophore that showed significant up-regulation and 4 significant down-regulations (p<0.05, fold change ≥2). The highest rate of up-regulation was observed in sodium/hydrogen exchanger,... (More)
Crayfish spermatophores are deposited on the body surface of the female during mating and remain there for a period of time before fertilization ensues. Post-mating changes in protein expression level in the noble crayfish Astacus astacus spermatophore were quantified. In-gel digestion and high resolution mass spectrometry were used for label-free protein quantification. One hundred twelve proteins were identified in the spermatophore of noble crayfish. After 7days of storage on the body of the female, 6 proteins were identified in the post-mating spermatophore that showed significant up-regulation and 4 significant down-regulations (p<0.05, fold change ≥2). The highest rate of up-regulation was observed in sodium/hydrogen exchanger, which may indicate the importance of intracellular pH adjustment for final maturation of the crayfish spermatozoon. The highest rate of down-regulation was observed in histone H2A. This may increase chromatin flexibility and facilitate its transfer into the oocyte during fertilization. The vitellogenin protein was identified in the crayfish spermatophore and its level changed during storage on the body surface of female. Extensive proteomic modification of male gametes during storage on the body surface of the female suggests post-mating final maturation of the crayfish spermatozoon. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Proteomics
volume
123
pages
70 - 77
publisher
Elsevier
external identifiers
  • pmid:25871631
  • wos:000356123700006
  • scopus:84928141046
ISSN
1874-3919
DOI
10.1016/j.jprot.2015.04.004
language
English
LU publication?
yes
id
c59dca51-6016-4ea5-b502-bdb374050127 (old id 5341923)
date added to LUP
2015-04-30 10:36:44
date last changed
2017-11-05 03:15:03
@article{c59dca51-6016-4ea5-b502-bdb374050127,
  abstract     = {Crayfish spermatophores are deposited on the body surface of the female during mating and remain there for a period of time before fertilization ensues. Post-mating changes in protein expression level in the noble crayfish Astacus astacus spermatophore were quantified. In-gel digestion and high resolution mass spectrometry were used for label-free protein quantification. One hundred twelve proteins were identified in the spermatophore of noble crayfish. After 7days of storage on the body of the female, 6 proteins were identified in the post-mating spermatophore that showed significant up-regulation and 4 significant down-regulations (p&lt;0.05, fold change ≥2). The highest rate of up-regulation was observed in sodium/hydrogen exchanger, which may indicate the importance of intracellular pH adjustment for final maturation of the crayfish spermatozoon. The highest rate of down-regulation was observed in histone H2A. This may increase chromatin flexibility and facilitate its transfer into the oocyte during fertilization. The vitellogenin protein was identified in the crayfish spermatophore and its level changed during storage on the body surface of female. Extensive proteomic modification of male gametes during storage on the body surface of the female suggests post-mating final maturation of the crayfish spermatozoon.},
  author       = {Niksirat, Hamid and James, Peter and Andersson, Liselotte and Kouba, Antonín and Kozák, Pavel},
  issn         = {1874-3919},
  language     = {eng},
  pages        = {70--77},
  publisher    = {Elsevier},
  series       = {Journal of Proteomics},
  title        = {Label-free protein quantification in freshly ejaculated versus post-mating spermatophores of the noble crayfish Astacus astacus.},
  url          = {http://dx.doi.org/10.1016/j.jprot.2015.04.004},
  volume       = {123},
  year         = {2015},
}