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Inbreeding affects gene expression differently in two self-incompatible Arabidopsis lyrata populations with similar levels of inbreeding depression.

Menzel, Mandy LU ; Sletvold, Nina; Ågren, Jon and Hansson, Bengt LU (2015) In Molecular Biology and Evolution 32(8). p.2036-2047
Abstract
Knowledge of which genes and pathways are affected by inbreeding may help understanding the genetic basis of inbreeding depression, the potential for purging (selection against deleterious recessive alleles) and the transition from outcrossing to selfing. Arabidopsis lyrata is a predominantly self-incompatible perennial plant, closely related to the selfing model species A. thaliana. To examine how inbreeding affects gene expression, we compared the transcriptome of experimentally selfed and outcrossed A. lyrata originating from two Scandinavian populations that express similar inbreeding depression for fitness (∂≈0.80). The number of genes significantly differentially expressed between selfed and outcrossed individuals were 2.5 times... (More)
Knowledge of which genes and pathways are affected by inbreeding may help understanding the genetic basis of inbreeding depression, the potential for purging (selection against deleterious recessive alleles) and the transition from outcrossing to selfing. Arabidopsis lyrata is a predominantly self-incompatible perennial plant, closely related to the selfing model species A. thaliana. To examine how inbreeding affects gene expression, we compared the transcriptome of experimentally selfed and outcrossed A. lyrata originating from two Scandinavian populations that express similar inbreeding depression for fitness (∂≈0.80). The number of genes significantly differentially expressed between selfed and outcrossed individuals were 2.5 times higher in the Norwegian population (≈500 genes) than in the Swedish population (≈200 genes). In both populations a majority of genes were up-regulated upon selfing (≈80%). Functional annotation analysis of the differentially expressed genes showed that selfed offspring were characterized by (i) up-regulation of stress-related genes in both populations, and (ii) up-regulation of photosynthesis-related genes in Sweden but down-regulation in Norway. Moreover, we found that reproduction- and pollination-related genes were affected by inbreeding only in Norway. We conclude that inbreeding causes both general and population-specific effects. The observed common effects suggest that inbreeding generally up-regulates rather than down-regulates gene expression and affects genes associated with stress response and general metabolic activity. Population differences in number of affected genes and in effects on the expression of photosynthesis-related genes show that the genetic basis of inbreeding depression can differ between populations with very similar levels of inbreeding depression. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Biology and Evolution
volume
32
issue
8
pages
2036 - 2047
publisher
Oxford University Press
external identifiers
  • pmid:25855783
  • wos:000360586500010
  • scopus:84964908543
ISSN
0737-4038
DOI
10.1093/molbev/msv086
project
Inbreeding and inbreeding depression
language
English
LU publication?
yes
id
4b3dfa4c-61a7-45ad-813b-32f4524c7f2a (old id 5345074)
date added to LUP
2015-04-30 10:27:57
date last changed
2017-08-13 03:04:38
@article{4b3dfa4c-61a7-45ad-813b-32f4524c7f2a,
  abstract     = {Knowledge of which genes and pathways are affected by inbreeding may help understanding the genetic basis of inbreeding depression, the potential for purging (selection against deleterious recessive alleles) and the transition from outcrossing to selfing. Arabidopsis lyrata is a predominantly self-incompatible perennial plant, closely related to the selfing model species A. thaliana. To examine how inbreeding affects gene expression, we compared the transcriptome of experimentally selfed and outcrossed A. lyrata originating from two Scandinavian populations that express similar inbreeding depression for fitness (∂≈0.80). The number of genes significantly differentially expressed between selfed and outcrossed individuals were 2.5 times higher in the Norwegian population (≈500 genes) than in the Swedish population (≈200 genes). In both populations a majority of genes were up-regulated upon selfing (≈80%). Functional annotation analysis of the differentially expressed genes showed that selfed offspring were characterized by (i) up-regulation of stress-related genes in both populations, and (ii) up-regulation of photosynthesis-related genes in Sweden but down-regulation in Norway. Moreover, we found that reproduction- and pollination-related genes were affected by inbreeding only in Norway. We conclude that inbreeding causes both general and population-specific effects. The observed common effects suggest that inbreeding generally up-regulates rather than down-regulates gene expression and affects genes associated with stress response and general metabolic activity. Population differences in number of affected genes and in effects on the expression of photosynthesis-related genes show that the genetic basis of inbreeding depression can differ between populations with very similar levels of inbreeding depression.},
  author       = {Menzel, Mandy and Sletvold, Nina and Ågren, Jon and Hansson, Bengt},
  issn         = {0737-4038},
  language     = {eng},
  number       = {8},
  pages        = {2036--2047},
  publisher    = {Oxford University Press},
  series       = {Molecular Biology and Evolution},
  title        = {Inbreeding affects gene expression differently in two self-incompatible Arabidopsis lyrata populations with similar levels of inbreeding depression.},
  url          = {http://dx.doi.org/10.1093/molbev/msv086},
  volume       = {32},
  year         = {2015},
}