Temperature limited fed-batch cultivation with a probing feeding strategy for Escherichia coli
(2004) p.73-79- Abstract
- A cultivation strategy combining the advantages of temperature limitedfed-batch and probing control is presented.This is a suitable way to produce recombinant proteins while minimizing therelease of endotoxins which complicates the downstream processing.The downside with the temperature limited fed-batch technique has been the difficulty to achieve a controlled excess of glucose inthe reactor without the accumulation of acetic acid when usingstandard sensors.A method using a probing feeding strategy withdown-pulses is here suggested. The technique is investigated insimulations and experiments.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/535975
- author
- de Maré, Lena LU ; Velut, Stéphane LU ; Briechle, Sebastian ; Wennerberg, Christina LU ; Cimander, Christian ; Ramchuran, Santosh LU ; Tunert, Pernilla ; Silfversparre, Gustav ; Holst, Olle LU and Hagander, Per LU
- organization
- publishing date
- 2004
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- temperature control, Escherichia coli, Fermentation process, feeding strategy
- host publication
- Computer Applications in Biotechnology 2004
- editor
- Pons, Marie-Noelle and Van Impe, Jan
- pages
- 73 - 79
- publisher
- International Federation of Automatic Control
- external identifiers
-
- scopus:85083219082
- ISBN
- 0-08-044251-X
- language
- English
- LU publication?
- yes
- id
- 19b6978f-b459-4c38-bc7b-eb5617f317cc (old id 535975)
- date added to LUP
- 2016-04-04 10:53:15
- date last changed
- 2022-01-29 20:58:36
@inproceedings{19b6978f-b459-4c38-bc7b-eb5617f317cc, abstract = {{A cultivation strategy combining the advantages of temperature limitedfed-batch and probing control is presented.This is a suitable way to produce recombinant proteins while minimizing therelease of endotoxins which complicates the downstream processing.The downside with the temperature limited fed-batch technique has been the difficulty to achieve a controlled excess of glucose inthe reactor without the accumulation of acetic acid when usingstandard sensors.A method using a probing feeding strategy withdown-pulses is here suggested. The technique is investigated insimulations and experiments.}}, author = {{de Maré, Lena and Velut, Stéphane and Briechle, Sebastian and Wennerberg, Christina and Cimander, Christian and Ramchuran, Santosh and Tunert, Pernilla and Silfversparre, Gustav and Holst, Olle and Hagander, Per}}, booktitle = {{Computer Applications in Biotechnology 2004}}, editor = {{Pons, Marie-Noelle and Van Impe, Jan}}, isbn = {{0-08-044251-X}}, keywords = {{temperature control; Escherichia coli; Fermentation process; feeding strategy}}, language = {{eng}}, pages = {{73--79}}, publisher = {{International Federation of Automatic Control}}, title = {{Temperature limited fed-batch cultivation with a probing feeding strategy for Escherichia coli}}, year = {{2004}}, }