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Temperature limited fed-batch cultivation with a probing feeding strategy for Escherichia coli

de Maré, Lena LU ; Velut, Stéphane LU ; Briechle, Sebastian; Wennerberg, Christina LU ; Cimander, Christian; Ramchuran, Santosh LU ; Tunert, Pernilla; Silfversparre, Gustav; Holst, Olle LU and Hagander, Per LU (2004) In Computer Applications in Biotechnology 2004 p.73-79
Abstract
A cultivation strategy combining the advantages of temperature limitedfed-batch and probing control is presented.This is a suitable way to produce recombinant proteins while minimizing therelease of endotoxins which complicates the downstream processing.The downside with the temperature limited fed-batch technique has been the difficulty to achieve a controlled excess of glucose inthe reactor without the accumulation of acetic acid when usingstandard sensors.A method using a probing feeding strategy withdown-pulses is here suggested. The technique is investigated insimulations and experiments.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
temperature control, Escherichia coli, Fermentation process, feeding strategy
in
Computer Applications in Biotechnology 2004
editor
Pons, Marie-Noelle; Van Impe, Jan; and
pages
73 - 79
publisher
International Federation of Automatic Control
ISBN
0-08-044251-X
language
English
LU publication?
yes
id
19b6978f-b459-4c38-bc7b-eb5617f317cc (old id 535975)
date added to LUP
2007-09-13 15:50:18
date last changed
2016-04-16 08:29:55
@inproceedings{19b6978f-b459-4c38-bc7b-eb5617f317cc,
  abstract     = {A cultivation strategy combining the advantages of temperature limitedfed-batch and probing control is presented.This is a suitable way to produce recombinant proteins while minimizing therelease of endotoxins which complicates the downstream processing.The downside with the temperature limited fed-batch technique has been the difficulty to achieve a controlled excess of glucose inthe reactor without the accumulation of acetic acid when usingstandard sensors.A method using a probing feeding strategy withdown-pulses is here suggested. The technique is investigated insimulations and experiments.},
  author       = {de Maré, Lena and Velut, Stéphane and Briechle, Sebastian and Wennerberg, Christina and Cimander, Christian and Ramchuran, Santosh and Tunert, Pernilla and Silfversparre, Gustav and Holst, Olle and Hagander, Per},
  booktitle    = {Computer Applications in Biotechnology 2004},
  editor       = {Pons, Marie-Noelle and Van Impe, Jan},
  isbn         = {0-08-044251-X},
  keyword      = {temperature control,Escherichia coli,Fermentation process,feeding strategy},
  language     = {eng},
  pages        = {73--79},
  publisher    = {International Federation of Automatic Control},
  title        = {Temperature limited fed-batch cultivation with a probing feeding strategy for Escherichia coli},
  year         = {2004},
}