Linoleic acid esters of hydroxy linoleic acids are anti-inflammatory lipids found in plants and mammals
(2019) In Journal of Biological Chemistry 294(27). p.10698-10707- Abstract
Fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of biologically active lipids. Here we identify the linoleic acid ester of 13-hydroxy linoleic acid (13-LAHLA) as an anti-inflammatory lipid. An oat oil fraction and FAHFA-enriched extract from this fraction showed anti-inflammatory activity in a lipopolysaccharide-induced cytokine secretion assay. Structural studies identified three LAHLA isomers (15-, 13-, and 9-LAHLA) as being the most abundant FAHFAs in the oat oil fraction. Of these LAHLAs, 13-LAHLA is the most abundant LAHLA isomer in human serum after ingestion of liposomes made of fractionated oat oil, and it is also the most abundant endogenous LAHLA in mouse and human adipose tissue. As a result,... (More)
Fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of biologically active lipids. Here we identify the linoleic acid ester of 13-hydroxy linoleic acid (13-LAHLA) as an anti-inflammatory lipid. An oat oil fraction and FAHFA-enriched extract from this fraction showed anti-inflammatory activity in a lipopolysaccharide-induced cytokine secretion assay. Structural studies identified three LAHLA isomers (15-, 13-, and 9-LAHLA) as being the most abundant FAHFAs in the oat oil fraction. Of these LAHLAs, 13-LAHLA is the most abundant LAHLA isomer in human serum after ingestion of liposomes made of fractionated oat oil, and it is also the most abundant endogenous LAHLA in mouse and human adipose tissue. As a result, we chemically synthesized 13-LAHLA for biological assays. 13-LAHLA suppresses lipopolysaccharide-stimulated secretion of cytokines and expression of pro-inflammatory genes. These studies identify LAHLAs as an evolutionarily conserved lipid with anti-inflammatory activity in mammalian cells.
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- author
- Kolar, Matthew J. ; Konduri, Srihari ; Chang, Tina ; Wang, Huijing ; McNerlin, Clare ; Ohlsson, Lena LU ; Härröd, Magnus ; Siegel, Dionicio and Saghatelian, Alan
- organization
- publishing date
- 2019-07-05
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biological Chemistry
- volume
- 294
- issue
- 27
- pages
- 10 pages
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- scopus:85069047335
- pmid:31152059
- ISSN
- 0021-9258
- DOI
- 10.1074/jbc.RA118.006956
- language
- English
- LU publication?
- yes
- id
- 535d7099-c6d2-4393-95ff-e7dc6a1e9d30
- date added to LUP
- 2019-07-26 16:23:41
- date last changed
- 2024-04-16 17:08:06
@article{535d7099-c6d2-4393-95ff-e7dc6a1e9d30, abstract = {{<p>Fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of biologically active lipids. Here we identify the linoleic acid ester of 13-hydroxy linoleic acid (13-LAHLA) as an anti-inflammatory lipid. An oat oil fraction and FAHFA-enriched extract from this fraction showed anti-inflammatory activity in a lipopolysaccharide-induced cytokine secretion assay. Structural studies identified three LAHLA isomers (15-, 13-, and 9-LAHLA) as being the most abundant FAHFAs in the oat oil fraction. Of these LAHLAs, 13-LAHLA is the most abundant LAHLA isomer in human serum after ingestion of liposomes made of fractionated oat oil, and it is also the most abundant endogenous LAHLA in mouse and human adipose tissue. As a result, we chemically synthesized 13-LAHLA for biological assays. 13-LAHLA suppresses lipopolysaccharide-stimulated secretion of cytokines and expression of pro-inflammatory genes. These studies identify LAHLAs as an evolutionarily conserved lipid with anti-inflammatory activity in mammalian cells.</p>}}, author = {{Kolar, Matthew J. and Konduri, Srihari and Chang, Tina and Wang, Huijing and McNerlin, Clare and Ohlsson, Lena and Härröd, Magnus and Siegel, Dionicio and Saghatelian, Alan}}, issn = {{0021-9258}}, language = {{eng}}, month = {{07}}, number = {{27}}, pages = {{10698--10707}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Journal of Biological Chemistry}}, title = {{Linoleic acid esters of hydroxy linoleic acids are anti-inflammatory lipids found in plants and mammals}}, url = {{http://dx.doi.org/10.1074/jbc.RA118.006956}}, doi = {{10.1074/jbc.RA118.006956}}, volume = {{294}}, year = {{2019}}, }