A single inlet two-stage acoustophoresis chip enabling tumor cell enrichment from white blood cells
Antfolk, Maria LU ; Antfolk, Christian LU ; Lilja, Hans LU
; Laurell, Thomas
LU
and Augustsson, Per
LU
(2015)
In Lab on a Chip
15(9).
p.2102-2109
- Abstract
- Metastatic disease is responsible for most cancer deaths, and hematogenous spread through circulating tumor cells (CTC) is a prerequisite for tumor dissemination. CTCs may undergo epithelial–mesenchymal transition where many epithelial cell characteristics are lost. Therefore, CTC isolation systems relying on epithelial cell markers are at risk of losing important subpopulations of cells. Here, a simple acoustophoresis-based cell separation instrument is presented. Cells are uniquely separated while maintained in their initial suspending medium, thus eliminating the need for a secondary cell-free medium to hydrodynamically pre-position them before the separation. When characterizing the system using polystyrene particles, 99.6 ± 0.2% of 7... (More)
- Metastatic disease is responsible for most cancer deaths, and hematogenous spread through circulating tumor cells (CTC) is a prerequisite for tumor dissemination. CTCs may undergo epithelial–mesenchymal transition where many epithelial cell characteristics are lost. Therefore, CTC isolation systems relying on epithelial cell markers are at risk of losing important subpopulations of cells. Here, a simple acoustophoresis-based cell separation instrument is presented. Cells are uniquely separated while maintained in their initial suspending medium, thus eliminating the need for a secondary cell-free medium to hydrodynamically pre-position them before the separation. When characterizing the system using polystyrene particles, 99.6 ± 0.2% of 7 μm diameter particles were collected through one outlet while 98.8 ± 0.5% of 5 μm particles were recovered through a second outlet. Prostate cancer cells (DU145) spiked into blood were enriched from white blood cells at a sample flow rate of 100 μL min−1 providing 86.5 ± 6.7% recovery of the cancer cells with 1.1 ± 0.2% contamination of white blood cells. By increasing the acoustic intensity a recovery of 94.8 ± 2.8% of cancer cells was achieved with 2.2 ± 0.6% contamination of white blood cells. The single inlet approach makes this instrument insensitive to acoustic impedance mismatch; a phenomenon reported to importantly affect accuracy in multi-laminar flow stream acoustophoresis. It also offers a possibility of concentrating the recovered cells in the chip, as opposed to systems relying on hydrodynamic pre-positioning which commonly dilute the target cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5364903
- author
- Antfolk, Maria
LU
; Antfolk, Christian
LU
; Lilja, Hans
LU
; Laurell, Thomas
LU
and Augustsson, Per
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Lab on a Chip
- volume
- 15
- issue
- 9
- pages
- 2102 - 2109
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000353219800017
- scopus:84928911612
- ISSN
- 1473-0189
- DOI
- 10.1039/C5LC00078E
- language
- English
- LU publication?
- yes
- id
- c40b5c80-3b79-475e-b95f-0574ec3de7f4 (old id 5364903)
- date added to LUP
- 2016-04-01 10:43:42
- date last changed
- 2022-05-18 01:30:12
@article{c40b5c80-3b79-475e-b95f-0574ec3de7f4,
abstract = {{Metastatic disease is responsible for most cancer deaths, and hematogenous spread through circulating tumor cells (CTC) is a prerequisite for tumor dissemination. CTCs may undergo epithelial–mesenchymal transition where many epithelial cell characteristics are lost. Therefore, CTC isolation systems relying on epithelial cell markers are at risk of losing important subpopulations of cells. Here, a simple acoustophoresis-based cell separation instrument is presented. Cells are uniquely separated while maintained in their initial suspending medium, thus eliminating the need for a secondary cell-free medium to hydrodynamically pre-position them before the separation. When characterizing the system using polystyrene particles, 99.6 ± 0.2% of 7 μm diameter particles were collected through one outlet while 98.8 ± 0.5% of 5 μm particles were recovered through a second outlet. Prostate cancer cells (DU145) spiked into blood were enriched from white blood cells at a sample flow rate of 100 μL min−1 providing 86.5 ± 6.7% recovery of the cancer cells with 1.1 ± 0.2% contamination of white blood cells. By increasing the acoustic intensity a recovery of 94.8 ± 2.8% of cancer cells was achieved with 2.2 ± 0.6% contamination of white blood cells. The single inlet approach makes this instrument insensitive to acoustic impedance mismatch; a phenomenon reported to importantly affect accuracy in multi-laminar flow stream acoustophoresis. It also offers a possibility of concentrating the recovered cells in the chip, as opposed to systems relying on hydrodynamic pre-positioning which commonly dilute the target cells.}},
author = {{Antfolk, Maria and Antfolk, Christian and Lilja, Hans and Laurell, Thomas and Augustsson, Per}},
issn = {{1473-0189}},
language = {{eng}},
number = {{9}},
pages = {{2102--2109}},
publisher = {{Royal Society of Chemistry}},
series = {{Lab on a Chip}},
title = {{A single inlet two-stage acoustophoresis chip enabling tumor cell enrichment from white blood cells}},
url = {{https://lup.lub.lu.se/search/files/2085749/8227643}},
doi = {{10.1039/C5LC00078E}},
volume = {{15}},
year = {{2015}},
}