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Characterization of the transcriptional and functional effects of fibroblast growth factor-1 on human preadipocyte differentiation

Newell, Felicity S. ; Su, Hua ; Tornqvist, Hans LU ; Whitehead, Jonathan P. ; Prins, Johannes B. and Hutley, Louise J. (2006) In FASEB Journal 20(14). p.2615-2615
Abstract
We recently established that fibroblast growth factor (FGF)-1 promotes adipogenesis of primary human preadipocytes (phPA). In the current report, we have characterized the adipogenic effects of FGF-1 in phPA and also in a human PA strain derived from an individual with Simpson-Golabi-Behmel syndrome (SGBS PA), which exhibit an intrinsic capacity to differentiate with high efficiency. In further studies, we compared these models with the well-characterized murine 3T3-L1 preadipocyte cell line (3T3-L1 PA). FGF-1 up-regulated the adipogenic program in phPA, with increased expression of peroxisome proliferator-activated receptor-gamma in confluent PA prior to induction of differentiation and increased expression of adipocyte markers during... (More)
We recently established that fibroblast growth factor (FGF)-1 promotes adipogenesis of primary human preadipocytes (phPA). In the current report, we have characterized the adipogenic effects of FGF-1 in phPA and also in a human PA strain derived from an individual with Simpson-Golabi-Behmel syndrome (SGBS PA), which exhibit an intrinsic capacity to differentiate with high efficiency. In further studies, we compared these models with the well-characterized murine 3T3-L1 preadipocyte cell line (3T3-L1 PA). FGF-1 up-regulated the adipogenic program in phPA, with increased expression of peroxisome proliferator-activated receptor-gamma in confluent PA prior to induction of differentiation and increased expression of adipocyte markers during differentiation. Moreover, phPA differentiated in the presence of FGF-1 were more insulin responsive and secreted increased levels of adiponectin. FGF-1 treatment of SGBS PA further enhanced differentiation. For the most part, the adipogenic program in phPA paralleled that observed in 3T3-L1 PA; however, we found no evidence of mitotic clonal expansion in the phPA. Finally, we investigated a role for extracellular regulated kinase 1/2 (ERK 1/2) in adipogenesis of phPA. FGF-1 induced robust phosphorylation of ERK1/2 in early differentiation and inhibition of ERK1/2 activity significantly reduced phPA differentiation. These data suggest that FGF-1 treated phPA represent a valuable in vitro model for the study of adipogenesis and insulin action and indicate that ERK1/2 activation is necessary for human adipogenesis in the absence of mitotic clonal expansion. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
obesity, adipogenesis, mitosis
in
FASEB Journal
volume
20
issue
14
pages
2615 - 2615
publisher
The Federation of American Societies for Experimental Biology
external identifiers
  • wos:000242490700042
ISSN
1530-6860
DOI
10.1096/fj.05-5710fje
language
English
LU publication?
yes
id
53d3ca4d-684d-49ff-9c77-005bb0bb267d (old id 685205)
date added to LUP
2016-04-01 16:19:34
date last changed
2019-12-28 04:40:16
@article{53d3ca4d-684d-49ff-9c77-005bb0bb267d,
  abstract     = {We recently established that fibroblast growth factor (FGF)-1 promotes adipogenesis of primary human preadipocytes (phPA). In the current report, we have characterized the adipogenic effects of FGF-1 in phPA and also in a human PA strain derived from an individual with Simpson-Golabi-Behmel syndrome (SGBS PA), which exhibit an intrinsic capacity to differentiate with high efficiency. In further studies, we compared these models with the well-characterized murine 3T3-L1 preadipocyte cell line (3T3-L1 PA). FGF-1 up-regulated the adipogenic program in phPA, with increased expression of peroxisome proliferator-activated receptor-gamma in confluent PA prior to induction of differentiation and increased expression of adipocyte markers during differentiation. Moreover, phPA differentiated in the presence of FGF-1 were more insulin responsive and secreted increased levels of adiponectin. FGF-1 treatment of SGBS PA further enhanced differentiation. For the most part, the adipogenic program in phPA paralleled that observed in 3T3-L1 PA; however, we found no evidence of mitotic clonal expansion in the phPA. Finally, we investigated a role for extracellular regulated kinase 1/2 (ERK 1/2) in adipogenesis of phPA. FGF-1 induced robust phosphorylation of ERK1/2 in early differentiation and inhibition of ERK1/2 activity significantly reduced phPA differentiation. These data suggest that FGF-1 treated phPA represent a valuable in vitro model for the study of adipogenesis and insulin action and indicate that ERK1/2 activation is necessary for human adipogenesis in the absence of mitotic clonal expansion.},
  author       = {Newell, Felicity S. and Su, Hua and Tornqvist, Hans and Whitehead, Jonathan P. and Prins, Johannes B. and Hutley, Louise J.},
  issn         = {1530-6860},
  language     = {eng},
  number       = {14},
  pages        = {2615--2615},
  publisher    = {The Federation of American Societies for Experimental Biology},
  series       = {FASEB Journal},
  title        = {Characterization of the transcriptional and functional effects of fibroblast growth factor-1 on human preadipocyte differentiation},
  url          = {http://dx.doi.org/10.1096/fj.05-5710fje},
  doi          = {10.1096/fj.05-5710fje},
  volume       = {20},
  year         = {2006},
}