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Increased cerebral uptake of [18F]fluoro-deoxyglucose but not [1-14C]glucose early following traumatic brain injury in rats

Marklund, Niklas LU orcid ; Sihver, Sven ; Hovda, David A ; Långström, Bengt ; Watanabe, Yasuyoshi ; Ronquist, Gunnar ; Bergström, Mats and Hillered, Lars (2009) In Journal of Neurotrauma 26(8). p.93-1281
Abstract

Following experimental and clinical traumatic brain injury (TBI), the local cerebral metabolic rate of glucose (lCMR(Glc)) is commonly estimated using the 2-[(18)F]fluoro-2-deoxy-D-glucose (FDG) method. The adequate estimation of lCMR(Glc) using FDG requires a correction factor, the lumped constant (LC), to convert FDG net uptake into lCMR(Glc). The LC, and thus lCMR(Glc) calculations, require a steady-state that may be disrupted following TBI. In the present report, we hypothesized that [1-(14)C]glucose uptake would accurately reflect glucose dynamics early post-injury, and was compared to the regional uptake of FDG in 44 rats subjected to moderate (2.4-2.6 atm) lateral fluid percussion brain injury (FPI) or sham injury. Cortical... (More)

Following experimental and clinical traumatic brain injury (TBI), the local cerebral metabolic rate of glucose (lCMR(Glc)) is commonly estimated using the 2-[(18)F]fluoro-2-deoxy-D-glucose (FDG) method. The adequate estimation of lCMR(Glc) using FDG requires a correction factor, the lumped constant (LC), to convert FDG net uptake into lCMR(Glc). The LC, and thus lCMR(Glc) calculations, require a steady-state that may be disrupted following TBI. In the present report, we hypothesized that [1-(14)C]glucose uptake would accurately reflect glucose dynamics early post-injury, and was compared to the regional uptake of FDG in 44 rats subjected to moderate (2.4-2.6 atm) lateral fluid percussion brain injury (FPI) or sham injury. Cortical energy state and adenylate (ATP, ADP, and AMP) levels were also measured. Early (7-42 min) after FPI, FDG uptake was increased in the ipsilateral cortex and hippocampus (p < 0.05). In contrast, no change in [1-(14)C]glucose uptake (7 and 17 min post-injury) or cortical adenylate content (42 min post-injury) was observed. At 12 h following FPI, the ipsilateral FDG and [1-(14)C]glucose uptake were decreased in the cortex and hippocampus, and the ipsilateral cortical ATP concentration was decreased in comparison to sham-injured controls (p < 0.05). Under the present experimental conditions, the rate of cerebral uptake of FDG and of [1-(14)C]glucose differed, and indicated that following TBI, regional changes in the LC may occur in the immediate, but not in the late, post-injury phase. These results should be considered when interpreting results obtained using FDG for the estimation of lCMR(Glc) early following experimental TBI.

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author
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publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Autoradiography, Brain Injuries, Brain Mapping, Cerebral Cortex, Energy Metabolism, Fluorodeoxyglucose F18, Glucose, Hippocampus, Male, Rats, Rats, Sprague-Dawley, Statistics, Nonparametric, Time Factors, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Neurotrauma
volume
26
issue
8
pages
93 - 1281
publisher
Mary Ann Liebert, Inc.
external identifiers
  • pmid:19355837
  • scopus:68349094924
ISSN
1557-9042
DOI
10.1089/neu.2008.0827
language
English
LU publication?
no
id
540bdc2a-0780-4951-931b-be566933075f
date added to LUP
2018-03-04 11:35:04
date last changed
2024-01-14 16:02:46
@article{540bdc2a-0780-4951-931b-be566933075f,
  abstract     = {{<p>Following experimental and clinical traumatic brain injury (TBI), the local cerebral metabolic rate of glucose (lCMR(Glc)) is commonly estimated using the 2-[(18)F]fluoro-2-deoxy-D-glucose (FDG) method. The adequate estimation of lCMR(Glc) using FDG requires a correction factor, the lumped constant (LC), to convert FDG net uptake into lCMR(Glc). The LC, and thus lCMR(Glc) calculations, require a steady-state that may be disrupted following TBI. In the present report, we hypothesized that [1-(14)C]glucose uptake would accurately reflect glucose dynamics early post-injury, and was compared to the regional uptake of FDG in 44 rats subjected to moderate (2.4-2.6 atm) lateral fluid percussion brain injury (FPI) or sham injury. Cortical energy state and adenylate (ATP, ADP, and AMP) levels were also measured. Early (7-42 min) after FPI, FDG uptake was increased in the ipsilateral cortex and hippocampus (p &lt; 0.05). In contrast, no change in [1-(14)C]glucose uptake (7 and 17 min post-injury) or cortical adenylate content (42 min post-injury) was observed. At 12 h following FPI, the ipsilateral FDG and [1-(14)C]glucose uptake were decreased in the cortex and hippocampus, and the ipsilateral cortical ATP concentration was decreased in comparison to sham-injured controls (p &lt; 0.05). Under the present experimental conditions, the rate of cerebral uptake of FDG and of [1-(14)C]glucose differed, and indicated that following TBI, regional changes in the LC may occur in the immediate, but not in the late, post-injury phase. These results should be considered when interpreting results obtained using FDG for the estimation of lCMR(Glc) early following experimental TBI.</p>}},
  author       = {{Marklund, Niklas and Sihver, Sven and Hovda, David A and Långström, Bengt and Watanabe, Yasuyoshi and Ronquist, Gunnar and Bergström, Mats and Hillered, Lars}},
  issn         = {{1557-9042}},
  keywords     = {{Animals; Autoradiography; Brain Injuries; Brain Mapping; Cerebral Cortex; Energy Metabolism; Fluorodeoxyglucose F18; Glucose; Hippocampus; Male; Rats; Rats, Sprague-Dawley; Statistics, Nonparametric; Time Factors; Journal Article; Research Support, Non-U.S. Gov't}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{93--1281}},
  publisher    = {{Mary Ann Liebert, Inc.}},
  series       = {{Journal of Neurotrauma}},
  title        = {{Increased cerebral uptake of [18F]fluoro-deoxyglucose but not [1-14C]glucose early following traumatic brain injury in rats}},
  url          = {{http://dx.doi.org/10.1089/neu.2008.0827}},
  doi          = {{10.1089/neu.2008.0827}},
  volume       = {{26}},
  year         = {{2009}},
}