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Moderate elevation of extracellular potassium transiently inhibits regeneration of sensory axons in cultured adult sciatic nerves

Edström, Anders LU ; Ekström, Per LU and Wiklund, Peter LU (1995) In Brain Research 693(1-2). p.148-154
Abstract

The adult frog dorsal root ganglia (DRG) together with the sciatic nerve (ScN) has previously been shown to survive in organ culture for several days. If a local test crush is made at the beginning of culturing, there is an initial delay of about 3 days before the sensory axons start to grow into the distal nerve stump at a rate of about 0.6-0.9 mm/day. The present results showed that axonal growth was unaffected in preparations maintained for 8 days in medium containing 10 mM K+ (5 mM is the physiological level). In contrast, the outgrowth was markedly reduced by 15 mM K+ and still more by 20 and 25 mM K+. The growth inhibition was partially counteracted by nifedipine, a Ca2+-channel... (More)

The adult frog dorsal root ganglia (DRG) together with the sciatic nerve (ScN) has previously been shown to survive in organ culture for several days. If a local test crush is made at the beginning of culturing, there is an initial delay of about 3 days before the sensory axons start to grow into the distal nerve stump at a rate of about 0.6-0.9 mm/day. The present results showed that axonal growth was unaffected in preparations maintained for 8 days in medium containing 10 mM K+ (5 mM is the physiological level). In contrast, the outgrowth was markedly reduced by 15 mM K+ and still more by 20 and 25 mM K+. The growth inhibition was partially counteracted by nifedipine, a Ca2+-channel antagonist. Other experiments clearly showed that high K+ exerted its effects during the early phase of the regeneration and lacked effects at later stages. The possibility that Ca2+-binding proteins, e.g., calbindin, which showed immunohistochemical reactivity in different structures, contribute to the growth adaptation to high K+ will be considered. The generality of the findings was supported by inhibition of axonal outgrowth of adult mouse sciatic sensory axons by high K+. © 1995 Elsevier Science B.V. All rights reserved.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Calbindin, Calcium, Potassium, Regeneration, Schwann cell, Sciatic nerve
in
Brain Research
volume
693
issue
1-2
pages
7 pages
publisher
Elsevier
external identifiers
  • Scopus:0029084681
ISSN
0006-8993
DOI
10.1016/0006-8993(95)00649-B
language
English
LU publication?
yes
id
54386ad8-f985-4723-ab4f-38314eb1fc3d
date added to LUP
2016-12-07 14:58:05
date last changed
2017-01-20 16:29:09
@article{54386ad8-f985-4723-ab4f-38314eb1fc3d,
  abstract     = {<p>The adult frog dorsal root ganglia (DRG) together with the sciatic nerve (ScN) has previously been shown to survive in organ culture for several days. If a local test crush is made at the beginning of culturing, there is an initial delay of about 3 days before the sensory axons start to grow into the distal nerve stump at a rate of about 0.6-0.9 mm/day. The present results showed that axonal growth was unaffected in preparations maintained for 8 days in medium containing 10 mM K<sup>+</sup> (5 mM is the physiological level). In contrast, the outgrowth was markedly reduced by 15 mM K<sup>+</sup> and still more by 20 and 25 mM K<sup>+</sup>. The growth inhibition was partially counteracted by nifedipine, a Ca<sup>2+</sup>-channel antagonist. Other experiments clearly showed that high K<sup>+</sup> exerted its effects during the early phase of the regeneration and lacked effects at later stages. The possibility that Ca<sup>2+</sup>-binding proteins, e.g., calbindin, which showed immunohistochemical reactivity in different structures, contribute to the growth adaptation to high K<sup>+</sup> will be considered. The generality of the findings was supported by inhibition of axonal outgrowth of adult mouse sciatic sensory axons by high K<sup>+</sup>. © 1995 Elsevier Science B.V. All rights reserved.</p>},
  author       = {Edström, Anders and Ekström, Per and Wiklund, Peter},
  issn         = {0006-8993},
  keyword      = {Calbindin,Calcium,Potassium,Regeneration,Schwann cell,Sciatic nerve},
  language     = {eng},
  month        = {09},
  number       = {1-2},
  pages        = {148--154},
  publisher    = {Elsevier},
  series       = {Brain Research},
  title        = {Moderate elevation of extracellular potassium transiently inhibits regeneration of sensory axons in cultured adult sciatic nerves},
  url          = {http://dx.doi.org/10.1016/0006-8993(95)00649-B},
  volume       = {693},
  year         = {1995},
}