Moderate elevation of extracellular potassium transiently inhibits regeneration of sensory axons in cultured adult sciatic nerves
(1995) In Brain Research 693(1-2). p.148-154- Abstract
The adult frog dorsal root ganglia (DRG) together with the sciatic nerve (ScN) has previously been shown to survive in organ culture for several days. If a local test crush is made at the beginning of culturing, there is an initial delay of about 3 days before the sensory axons start to grow into the distal nerve stump at a rate of about 0.6-0.9 mm/day. The present results showed that axonal growth was unaffected in preparations maintained for 8 days in medium containing 10 mM K+ (5 mM is the physiological level). In contrast, the outgrowth was markedly reduced by 15 mM K+ and still more by 20 and 25 mM K+. The growth inhibition was partially counteracted by nifedipine, a Ca2+-channel... (More)
The adult frog dorsal root ganglia (DRG) together with the sciatic nerve (ScN) has previously been shown to survive in organ culture for several days. If a local test crush is made at the beginning of culturing, there is an initial delay of about 3 days before the sensory axons start to grow into the distal nerve stump at a rate of about 0.6-0.9 mm/day. The present results showed that axonal growth was unaffected in preparations maintained for 8 days in medium containing 10 mM K+ (5 mM is the physiological level). In contrast, the outgrowth was markedly reduced by 15 mM K+ and still more by 20 and 25 mM K+. The growth inhibition was partially counteracted by nifedipine, a Ca2+-channel antagonist. Other experiments clearly showed that high K+ exerted its effects during the early phase of the regeneration and lacked effects at later stages. The possibility that Ca2+-binding proteins, e.g., calbindin, which showed immunohistochemical reactivity in different structures, contribute to the growth adaptation to high K+ will be considered. The generality of the findings was supported by inhibition of axonal outgrowth of adult mouse sciatic sensory axons by high K+.
(Less)
- author
- Edström, Anders LU ; Ekström, Per LU and Wiklund, Peter LU
- organization
- publishing date
- 1995-09-25
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Calbindin, Calcium, Potassium, Regeneration, Schwann cell, Sciatic nerve
- in
- Brain Research
- volume
- 693
- issue
- 1-2
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:8653402
- scopus:0029084681
- ISSN
- 0006-8993
- DOI
- 10.1016/0006-8993(95)00649-B
- language
- English
- LU publication?
- yes
- id
- 54386ad8-f985-4723-ab4f-38314eb1fc3d
- date added to LUP
- 2016-12-07 14:58:05
- date last changed
- 2024-01-04 18:24:48
@article{54386ad8-f985-4723-ab4f-38314eb1fc3d, abstract = {{<p>The adult frog dorsal root ganglia (DRG) together with the sciatic nerve (ScN) has previously been shown to survive in organ culture for several days. If a local test crush is made at the beginning of culturing, there is an initial delay of about 3 days before the sensory axons start to grow into the distal nerve stump at a rate of about 0.6-0.9 mm/day. The present results showed that axonal growth was unaffected in preparations maintained for 8 days in medium containing 10 mM K<sup>+</sup> (5 mM is the physiological level). In contrast, the outgrowth was markedly reduced by 15 mM K<sup>+</sup> and still more by 20 and 25 mM K<sup>+</sup>. The growth inhibition was partially counteracted by nifedipine, a Ca<sup>2+</sup>-channel antagonist. Other experiments clearly showed that high K<sup>+</sup> exerted its effects during the early phase of the regeneration and lacked effects at later stages. The possibility that Ca<sup>2+</sup>-binding proteins, e.g., calbindin, which showed immunohistochemical reactivity in different structures, contribute to the growth adaptation to high K<sup>+</sup> will be considered. The generality of the findings was supported by inhibition of axonal outgrowth of adult mouse sciatic sensory axons by high K<sup>+</sup>.</p>}}, author = {{Edström, Anders and Ekström, Per and Wiklund, Peter}}, issn = {{0006-8993}}, keywords = {{Calbindin; Calcium; Potassium; Regeneration; Schwann cell; Sciatic nerve}}, language = {{eng}}, month = {{09}}, number = {{1-2}}, pages = {{148--154}}, publisher = {{Elsevier}}, series = {{Brain Research}}, title = {{Moderate elevation of extracellular potassium transiently inhibits regeneration of sensory axons in cultured adult sciatic nerves}}, url = {{http://dx.doi.org/10.1016/0006-8993(95)00649-B}}, doi = {{10.1016/0006-8993(95)00649-B}}, volume = {{693}}, year = {{1995}}, }