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Oxidative stress, telomere shortening, and DNA methylation in relation to low-to-moderate occupational exposure to welding fumes.

Li, Huiqi LU ; Hedmer, Maria LU ; Wojdacz, Tomasz K LU ; Hossain, Bakhtiar LU ; Lindh, Christian LU ; Tinnerberg, Håkan LU ; Albin, Maria LU and Broberg Palmgren, Karin LU (2015) In Environmental and Molecular Mutagenesis 56(8). p.684-693
Abstract
Evidence suggests that exposure to welding fumes is a risk factor for lung cancer. We examined relationships between low-to-moderate occupational exposure to particles from welding fumes and cancer-related biomarkers for oxidative stress, changes in telomere length, and alterations in DNA methylation. We enrolled 101 welders and 127 controls (all currently nonsmoking men) from southern Sweden. We performed personal sampling of respirable dust and measured 8-oxodG concentrations in urine using a simplified liquid chromatography tandem mass spectrometry method. Telomere length in peripheral blood was measured by quantitative polymerase chain reaction. Methylation status of 10 tumor suppressor genes was determined by methylation-sensitive... (More)
Evidence suggests that exposure to welding fumes is a risk factor for lung cancer. We examined relationships between low-to-moderate occupational exposure to particles from welding fumes and cancer-related biomarkers for oxidative stress, changes in telomere length, and alterations in DNA methylation. We enrolled 101 welders and 127 controls (all currently nonsmoking men) from southern Sweden. We performed personal sampling of respirable dust and measured 8-oxodG concentrations in urine using a simplified liquid chromatography tandem mass spectrometry method. Telomere length in peripheral blood was measured by quantitative polymerase chain reaction. Methylation status of 10 tumor suppressor genes was determined by methylation-sensitive high-resolution melting analysis. All analyses were adjusted for age, body mass index, previous smoking, passive smoking, current residence, and wood burning stove/boiler at home. Welders were exposed to respirable dust at 1.2 mg/m(3) (standard deviation, 3.3 mg/m(3) ; range, 0.1-19.3), whereas control exposures did not exceed 0.1 mg/m(3) (P < 0.001). Welders and controls did not differ in 8-oxodG levels (β = 1.2, P = 0.17) or relative telomere length (β = -0.053, P = 0.083) in adjusted models. Welders showed higher probability of adenomatous polyposis coli (APC) methylation in the unadjusted model (odds ratio = 14, P = 0.014), but this was not significant in the fully adjusted model (P = 0.052). Every working year as a welder was associated with 0.0066 units shorter telomeres (95% confidence interval -0.013 to -0.00053, P = 0.033). Although there were no clear associations between concentrations of respirable dust and the biomarkers, there were modest signs of associations between oxidative stress, telomere alterations, DNA methylation, and occupational exposure to low-to-moderate levels of particles. Environ. Mol. Mutagen., 2015. © 2015 Wiley Periodicals, Inc. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Environmental and Molecular Mutagenesis
volume
56
issue
8
pages
684 - 693
publisher
John Wiley & Sons
external identifiers
  • pmid:26013103
  • wos:000363209100005
  • scopus:84944274721
ISSN
1098-2280
DOI
10.1002/em.21958
language
English
LU publication?
yes
id
5fc14e9b-102b-4f04-890d-c9b305ff3011 (old id 5442169)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26013103?dopt=Abstract
date added to LUP
2015-06-05 15:27:21
date last changed
2017-07-02 03:12:01
@article{5fc14e9b-102b-4f04-890d-c9b305ff3011,
  abstract     = {Evidence suggests that exposure to welding fumes is a risk factor for lung cancer. We examined relationships between low-to-moderate occupational exposure to particles from welding fumes and cancer-related biomarkers for oxidative stress, changes in telomere length, and alterations in DNA methylation. We enrolled 101 welders and 127 controls (all currently nonsmoking men) from southern Sweden. We performed personal sampling of respirable dust and measured 8-oxodG concentrations in urine using a simplified liquid chromatography tandem mass spectrometry method. Telomere length in peripheral blood was measured by quantitative polymerase chain reaction. Methylation status of 10 tumor suppressor genes was determined by methylation-sensitive high-resolution melting analysis. All analyses were adjusted for age, body mass index, previous smoking, passive smoking, current residence, and wood burning stove/boiler at home. Welders were exposed to respirable dust at 1.2 mg/m(3) (standard deviation, 3.3 mg/m(3) ; range, 0.1-19.3), whereas control exposures did not exceed 0.1 mg/m(3) (P &lt; 0.001). Welders and controls did not differ in 8-oxodG levels (β = 1.2, P = 0.17) or relative telomere length (β = -0.053, P = 0.083) in adjusted models. Welders showed higher probability of adenomatous polyposis coli (APC) methylation in the unadjusted model (odds ratio = 14, P = 0.014), but this was not significant in the fully adjusted model (P = 0.052). Every working year as a welder was associated with 0.0066 units shorter telomeres (95% confidence interval -0.013 to -0.00053, P = 0.033). Although there were no clear associations between concentrations of respirable dust and the biomarkers, there were modest signs of associations between oxidative stress, telomere alterations, DNA methylation, and occupational exposure to low-to-moderate levels of particles. Environ. Mol. Mutagen., 2015. © 2015 Wiley Periodicals, Inc.},
  author       = {Li, Huiqi and Hedmer, Maria and Wojdacz, Tomasz K and Hossain, Bakhtiar and Lindh, Christian and Tinnerberg, Håkan and Albin, Maria and Broberg Palmgren, Karin},
  issn         = {1098-2280},
  language     = {eng},
  number       = {8},
  pages        = {684--693},
  publisher    = {John Wiley & Sons},
  series       = {Environmental and Molecular Mutagenesis},
  title        = {Oxidative stress, telomere shortening, and DNA methylation in relation to low-to-moderate occupational exposure to welding fumes.},
  url          = {http://dx.doi.org/10.1002/em.21958},
  volume       = {56},
  year         = {2015},
}