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TNFalpha expression in monocytes/macrophages

Andersson, Karolina LU (2005) In Lund University Faculty of Medicine Doctoral Dissertation Series
Abstract
The recognition of microbes or microbial products by leukocytes is a key event in innate immunity. Binding of microbial products to cell surface receptors on phagocytes induce a response that includes the production of inflammatory mediators. One such mediator is the cytokine tumour necrosis factor alpha (TNF). TNF is a pleotropic cytokine with actions ranging from tissue injury to septic chock. This thesis is based on studies performed with the use of different stimuli (either bacteria, the bacterial product LPS or direct activators of protein kinases) which induce TNF production in cells of the monocytic lineage. The main focus has been to study the signal transduction pathway regulating TNF expression.



Dexamethasone,... (More)
The recognition of microbes or microbial products by leukocytes is a key event in innate immunity. Binding of microbial products to cell surface receptors on phagocytes induce a response that includes the production of inflammatory mediators. One such mediator is the cytokine tumour necrosis factor alpha (TNF). TNF is a pleotropic cytokine with actions ranging from tissue injury to septic chock. This thesis is based on studies performed with the use of different stimuli (either bacteria, the bacterial product LPS or direct activators of protein kinases) which induce TNF production in cells of the monocytic lineage. The main focus has been to study the signal transduction pathway regulating TNF expression.



Dexamethasone, a synthetic glucocorticoid, inhibits bacteria-induced TNF production in mouse macrophages. Effects on TNF expression when cells are pretreated with dexamethasone are a decrease in gene transcription as well as posttranscriptional inhibition. Okadaic acid, a protein phosphatase inhibitor, seems to overcome the posttranscriptional inhibition exerted by dexamethasone when TNF expression is induced.



The posttranscriptional regulation of TNF was further studied in human monocytic THP-1 cells. THP-1 cells express TNF mRNA when unstimulated, but produce no TNF protein. Induction of TNF production can be achieved by activation of the classical MAP kinase or p38 MAP kinase pathways without any significant change in gene transcription.



TNF mRNA expression is not detectable in primary mouse macrophages but when challenged with LPS, TNF mRNA and subsequent protein production increases. TNF production is regulated both at the level of gene transcription and translation of the transcripts. The kinase Mnk1, activated by ERK and/or p38, phosphorylates the translation initiation factor eIF4E and this correlates with TNF production. A proposed signalling pathway to translational regulation of TNF in mouse macrophages is presented. (Less)
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author
supervisor
opponent
  • Associate Professor Rasmusson, Birgitta, Department of Molecular and Clinical Medicine,University Hospital of Linköping
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Biomedicinska vetenskaper (allmänt), General biomedical sciences, Protein kinase, Macrophage, TNF
in
Lund University Faculty of Medicine Doctoral Dissertation Series
pages
96 pages
publisher
Department of Experimental Medical Science, Lund Univeristy
defense location
Grubb-salen, BMC, Sölvegatan 19, Lund
defense date
2005-05-19 09:15
ISSN
1652-8220
ISBN
91-628-6507-2
language
English
LU publication?
yes
id
9ae7b46d-69d4-4b77-b01f-9bc63ced40ff (old id 544826)
date added to LUP
2007-09-07 15:37:12
date last changed
2016-09-19 08:44:55
@phdthesis{9ae7b46d-69d4-4b77-b01f-9bc63ced40ff,
  abstract     = {The recognition of microbes or microbial products by leukocytes is a key event in innate immunity. Binding of microbial products to cell surface receptors on phagocytes induce a response that includes the production of inflammatory mediators. One such mediator is the cytokine tumour necrosis factor alpha (TNF). TNF is a pleotropic cytokine with actions ranging from tissue injury to septic chock. This thesis is based on studies performed with the use of different stimuli (either bacteria, the bacterial product LPS or direct activators of protein kinases) which induce TNF production in cells of the monocytic lineage. The main focus has been to study the signal transduction pathway regulating TNF expression.<br/><br>
<br/><br>
Dexamethasone, a synthetic glucocorticoid, inhibits bacteria-induced TNF production in mouse macrophages. Effects on TNF expression when cells are pretreated with dexamethasone are a decrease in gene transcription as well as posttranscriptional inhibition. Okadaic acid, a protein phosphatase inhibitor, seems to overcome the posttranscriptional inhibition exerted by dexamethasone when TNF expression is induced.<br/><br>
<br/><br>
The posttranscriptional regulation of TNF was further studied in human monocytic THP-1 cells. THP-1 cells express TNF mRNA when unstimulated, but produce no TNF protein. Induction of TNF production can be achieved by activation of the classical MAP kinase or p38 MAP kinase pathways without any significant change in gene transcription.<br/><br>
<br/><br>
TNF mRNA expression is not detectable in primary mouse macrophages but when challenged with LPS, TNF mRNA and subsequent protein production increases. TNF production is regulated both at the level of gene transcription and translation of the transcripts. The kinase Mnk1, activated by ERK and/or p38, phosphorylates the translation initiation factor eIF4E and this correlates with TNF production. A proposed signalling pathway to translational regulation of TNF in mouse macrophages is presented.},
  author       = {Andersson, Karolina},
  isbn         = {91-628-6507-2},
  issn         = {1652-8220},
  keyword      = {Biomedicinska vetenskaper (allmänt),General biomedical sciences,Protein kinase,Macrophage,TNF},
  language     = {eng},
  pages        = {96},
  publisher    = {Department of Experimental Medical Science, Lund Univeristy},
  school       = {Lund University},
  series       = {Lund University Faculty of Medicine Doctoral Dissertation Series},
  title        = {TNFalpha expression in monocytes/macrophages},
  year         = {2005},
}