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Development of a Bioelectronic Tongue -Applications for Wastewater Analysis

Dock, Eva LU (2006)
Abstract
A bioelectronic tongue has been developed for applications in wastewater analysis. The development of a biosensor array with complex signal analysis started from the idea of using group-selective phenol biosensors (tyrosinase and horseradish peroxidase) with chemometric analysis for signal processing. In a first step the ability to simultaneously determine each analyte in synthetic binary phenol mixtures was evaluated using multivariate data analysis on the responses from a single tyrosinase-modified solid graphite electrode. The next step was to construct a suitable device where different biosensors could be used in an array for multi-parameter detection of samples. Screen-printed electrodes of carbon and noble metals were first evaluated... (More)
A bioelectronic tongue has been developed for applications in wastewater analysis. The development of a biosensor array with complex signal analysis started from the idea of using group-selective phenol biosensors (tyrosinase and horseradish peroxidase) with chemometric analysis for signal processing. In a first step the ability to simultaneously determine each analyte in synthetic binary phenol mixtures was evaluated using multivariate data analysis on the responses from a single tyrosinase-modified solid graphite electrode. The next step was to construct a suitable device where different biosensors could be used in an array for multi-parameter detection of samples. Screen-printed electrodes of carbon and noble metals were first evaluated with the purpose of identifying electrodes that could be used as a basis for immobilisation of phenol- and pesticide-sensitive enzymes (horseradish peroxidase, soybean peroxidase, cellobiose dehydrogenase, acetylcholinesterase and butyrylcholinesterase). These enzymes were then immobilised on an array of eight radially distributed electrodes. To host the array and to provide equal hydrodynamics at each electrode in the array, a special electrochemical cell was constructed to enable flow-injection and steady-state measurements. Together with multivariate data analysis has this array system been successfully used for qualitative discrimination of wastewater samples as well as for quantitative determination of their toxicity and other pollution parameters such as chemical oxygen demand and biological oxygen demand. Pre-processing of data before multivariate analysis was shown to be necessary for reducing the noise that otherwise can hide the desired structural information. Several strategies to overcome noise problems due to drift in biosensors have been developed. (Less)
Please use this url to cite or link to this publication:
author
supervisor
opponent
  • Professor Winquist, Fredrik, S-SENCE and Laboratory of Applied Physics, Linköpings Universitet, Sverige
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Analytisk kemi, screen-printed electrode, multivariate data analysis, wastewater, Analytical chemistry, electronic tongue, biosensor array, amperometric
pages
164 pages
publisher
Department of Analytical Chemistry, Lund University
defense location
Lunds universitet, Kemicentrum, Sölvegatan 39, hörsal A
defense date
2006-06-02 13:15:00
ISBN
91-628-6854-3
language
English
LU publication?
yes
additional info
Eva Dock, Jakob Christensen, Mattias Olsson, Erik Tonning, Tautgirdas Ruzgas and Jenny Emnéus. 2005. Multivariate Data Analysis of Dynamic Amperometric Biosensor Responses from Binary Analyte Mixtures - Application of Sensitivity Correction Algorithms Talanta, vol 65 pp 298-305. Elsevier
Eva Dock, Andreas Christenson, Svetlana Sapelnikova, Jan Krejci, Jenny Emnéus and Tautgirdas Ruzgas. 2005. A Steady-State and Flow-Through Cell for Screen-Printed Eight-Electrode Arrays Analytica Chimica Acta, vol 531 pp 165-172. Elsevier
Renata Solná, Eva Dock, Andreas Christenson, Margrethe Winther-Nielsen, Charlotte Carlsson, Jenny Emnéus, Tautgirdas Ruzgas and Petr Skládal. 2005. Amperometric Screen-Printed Biosensor Arrays with Co-Immobilised Oxidoreductases and Cholinesterases Analytica Chimica Acta, vol 528 pp 9-19. Elsevier
Erik Tonning, Svetlana Sapelnikova, Jakob Christensen, Charlotte Carlsson, Margrethe Winther-Nielsen, Eva Dock, Renata Solná, Petr Skládal, Lars Norgaard, Tautgirdas Ruzgas and Jenny Emnéus. 2005. Chemometric Exploration of an Amperometric Biosensor Array for Fast Determination of Wastewater Quality Biosensors and Bioelectronics, vol 21 pp 608-617. Elsevier
Eva Dock, Annika Lindgren, Tautgirdas Ruzgas and Lo Gorton. 2001. Effect of Interfering Substances on Current Response of Recombinant Peroxidase and Glucose Oxidase-Recombinant Peroxidase Modified Graphite Electrodes Analyst, vol 126 pp 1929-1935. The Royal Society of Chemistry
Eva Dock and Tautgirdas Ruzgas. 2003. Screen-Printed Carbon Electrodes Modified with Cellobiose Dehydrogenase: Amplification Factor for Catechol vs. Reversibility of Ferricyanide Electroanalysis, vol 15 pp 492-498. Wiley
Eva Dock, Erik Tonning, Jakob Christensen, Margrethe Winther-Nielsen, Charlotte Carlsson, Renata Solná, Petr Skládal, Lars Norgaard, Jenny Emnéus and Tautgirdas Ruzgas. 2006. An amperometric bioelectronic tongue for simultaneous determination of toxicity, COD and BOD in wastewater (manuscript)
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
37c34132-d33b-45be-b3c0-51271ad3b832 (old id 546828)
date added to LUP
2016-04-04 09:54:07
date last changed
2024-07-02 13:21:06
@phdthesis{37c34132-d33b-45be-b3c0-51271ad3b832,
  abstract     = {{A bioelectronic tongue has been developed for applications in wastewater analysis. The development of a biosensor array with complex signal analysis started from the idea of using group-selective phenol biosensors (tyrosinase and horseradish peroxidase) with chemometric analysis for signal processing. In a first step the ability to simultaneously determine each analyte in synthetic binary phenol mixtures was evaluated using multivariate data analysis on the responses from a single tyrosinase-modified solid graphite electrode. The next step was to construct a suitable device where different biosensors could be used in an array for multi-parameter detection of samples. Screen-printed electrodes of carbon and noble metals were first evaluated with the purpose of identifying electrodes that could be used as a basis for immobilisation of phenol- and pesticide-sensitive enzymes (horseradish peroxidase, soybean peroxidase, cellobiose dehydrogenase, acetylcholinesterase and butyrylcholinesterase). These enzymes were then immobilised on an array of eight radially distributed electrodes. To host the array and to provide equal hydrodynamics at each electrode in the array, a special electrochemical cell was constructed to enable flow-injection and steady-state measurements. Together with multivariate data analysis has this array system been successfully used for qualitative discrimination of wastewater samples as well as for quantitative determination of their toxicity and other pollution parameters such as chemical oxygen demand and biological oxygen demand. Pre-processing of data before multivariate analysis was shown to be necessary for reducing the noise that otherwise can hide the desired structural information. Several strategies to overcome noise problems due to drift in biosensors have been developed.}},
  author       = {{Dock, Eva}},
  isbn         = {{91-628-6854-3}},
  keywords     = {{Analytisk kemi; screen-printed electrode; multivariate data analysis; wastewater; Analytical chemistry; electronic tongue; biosensor array; amperometric}},
  language     = {{eng}},
  publisher    = {{Department of Analytical Chemistry, Lund University}},
  school       = {{Lund University}},
  title        = {{Development of a Bioelectronic Tongue -Applications for Wastewater Analysis}},
  url          = {{https://lup.lub.lu.se/search/files/5415792/546835.pdf}},
  year         = {{2006}},
}