Npas4, a novel helix-loop-helix PAS domain protein, is regulated in response to cerebral ischemia
(2006) In European Journal of Neuroscience 24(10). p.2705-2720- Abstract
- Basic helix-loop-helix PAS domain proteins form a growing family of transcription factors. These proteins are involved in the process of adaptation to cellular stresses and environmental factors such as a change in oxygen concentration. We describe the identification and characterization of a recently cloned PAS domain protein termed Npas4 in ischemic rat brain. Using gene expression profiling following middle cerebral artery occlusion, we showed that the Npas4 mRNA is differentially expressed in ischemic tissue. The full-length gene was cloned from rat brain and its spatial and temporal expression characterized with in situ hybridization and Northern blotting. The Npas4 mRNA is specifically expressed in the brain and is highly... (More)
- Basic helix-loop-helix PAS domain proteins form a growing family of transcription factors. These proteins are involved in the process of adaptation to cellular stresses and environmental factors such as a change in oxygen concentration. We describe the identification and characterization of a recently cloned PAS domain protein termed Npas4 in ischemic rat brain. Using gene expression profiling following middle cerebral artery occlusion, we showed that the Npas4 mRNA is differentially expressed in ischemic tissue. The full-length gene was cloned from rat brain and its spatial and temporal expression characterized with in situ hybridization and Northern blotting. The Npas4 mRNA is specifically expressed in the brain and is highly up-regulated in ischemic tissues following both focal and global cerebral ischemic insults. Immunohistochemistry revealed a strong expression in the limbic system and thalamus, as well as in layers 3 and 5 in the cortex of the unchallenged brain. When overexpressed in HEK 293 cells, Npas4 appears as a protein of similar to 100 kDa. In brain samples, however, in addition to the 100 kDa band a specific 200 kDa immunoreactive band was also detected. Ischemic challenge lead to a decrease in the 200 kDa form and a simultaneous increase in the 100 kDa immunoreactivity. This could indicate a novel regulatory mechanism for activation and/or deactivation of this protein in response to ischemic brain injury. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/376618
- author
- Shamloo, Mehrdad ; Soriano, Liza ; von Schack, David ; Rickhag, Mattias LU ; Chin, Daniel J. ; Gonzalez-Zulueta, Mirella ; Gidö, Gunilla LU ; Urfer, Roman ; Wieloch, Tadeusz LU and Nikolich, Karoly
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- rat, NXF, hypoxia-inducing factor 1 alpha, brain, hypoxia, stroke
- in
- European Journal of Neuroscience
- volume
- 24
- issue
- 10
- pages
- 2705 - 2720
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000242221700003
- scopus:33751360974
- pmid:17156197
- ISSN
- 1460-9568
- DOI
- 10.1111/j.1460-9568.2006.05172.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Laboratory for Experimental Brain Research (013041000)
- id
- 54a1778c-c842-44e0-98f8-fdbd2fc76d88 (old id 376618)
- date added to LUP
- 2016-04-01 11:43:09
- date last changed
- 2022-01-26 17:12:07
@article{54a1778c-c842-44e0-98f8-fdbd2fc76d88,
abstract = {{Basic helix-loop-helix PAS domain proteins form a growing family of transcription factors. These proteins are involved in the process of adaptation to cellular stresses and environmental factors such as a change in oxygen concentration. We describe the identification and characterization of a recently cloned PAS domain protein termed Npas4 in ischemic rat brain. Using gene expression profiling following middle cerebral artery occlusion, we showed that the Npas4 mRNA is differentially expressed in ischemic tissue. The full-length gene was cloned from rat brain and its spatial and temporal expression characterized with in situ hybridization and Northern blotting. The Npas4 mRNA is specifically expressed in the brain and is highly up-regulated in ischemic tissues following both focal and global cerebral ischemic insults. Immunohistochemistry revealed a strong expression in the limbic system and thalamus, as well as in layers 3 and 5 in the cortex of the unchallenged brain. When overexpressed in HEK 293 cells, Npas4 appears as a protein of similar to 100 kDa. In brain samples, however, in addition to the 100 kDa band a specific 200 kDa immunoreactive band was also detected. Ischemic challenge lead to a decrease in the 200 kDa form and a simultaneous increase in the 100 kDa immunoreactivity. This could indicate a novel regulatory mechanism for activation and/or deactivation of this protein in response to ischemic brain injury.}},
author = {{Shamloo, Mehrdad and Soriano, Liza and von Schack, David and Rickhag, Mattias and Chin, Daniel J. and Gonzalez-Zulueta, Mirella and Gidö, Gunilla and Urfer, Roman and Wieloch, Tadeusz and Nikolich, Karoly}},
issn = {{1460-9568}},
keywords = {{rat; NXF; hypoxia-inducing factor 1 alpha; brain; hypoxia; stroke}},
language = {{eng}},
number = {{10}},
pages = {{2705--2720}},
publisher = {{Wiley-Blackwell}},
series = {{European Journal of Neuroscience}},
title = {{Npas4, a novel helix-loop-helix PAS domain protein, is regulated in response to cerebral ischemia}},
url = {{http://dx.doi.org/10.1111/j.1460-9568.2006.05172.x}},
doi = {{10.1111/j.1460-9568.2006.05172.x}},
volume = {{24}},
year = {{2006}},
}