Stimulation of blood mononuclear cells with bacterial virulence factors leads to the release of pro-coagulant and pro-inflammatory microparticles.
Oehmcke, Sonja LU ; Mörgelin, Matthias LU ; Malmström, Johan ; Linder, Adam LU ; Chew, Michelle LU ; Thorlacius, Henrik LU and Herwald, Heiko LU
(2012)
In Cellular Microbiology
14.
p.107-119
- Abstract
- Severe infectious diseases remain a major and life-threatening health problem. In serious cases a systemic activation of the coagulation cascade and hypovolemic shock are critical complications that are associated with high mortality rates. Here we report that blood mononuclear cells, stimulated with M1 protein of Streptococcus pyogenes or other bacterial virulence factors, produce not only pro-coagulant, but also pro-inflammatory microparticles (MPs). Our results also show that activation of the contact system on MPs contributes to these two effects. Phosphatidylserine (PS) plays an important role in these processes as its up-regulation on MPs allows an assembly and activation of the contact system. This in turn results in stabilization... (More)
- Severe infectious diseases remain a major and life-threatening health problem. In serious cases a systemic activation of the coagulation cascade and hypovolemic shock are critical complications that are associated with high mortality rates. Here we report that blood mononuclear cells, stimulated with M1 protein of Streptococcus pyogenes or other bacterial virulence factors, produce not only pro-coagulant, but also pro-inflammatory microparticles (MPs). Our results also show that activation of the contact system on MPs contributes to these two effects. Phosphatidylserine (PS) plays an important role in these processes as its up-regulation on MPs allows an assembly and activation of the contact system. This in turn results in stabilization of the tissue factor-induced clot and a processing of high-molecular weight kininogen by plasma kallikrein followed by the release of bradykinin, a potent vascular mediator. Pro-coagulant monocyte-derived MPs were identified in plasma samples from septic patients and further analysis of MPs from these patients revealed that their pro-coagulant activity is dependent on the tissue factor- and contact system-driven pathway. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2168362
- author
- Oehmcke, Sonja
LU
; Mörgelin, Matthias
LU
; Malmström, Johan
; Linder, Adam
LU
; Chew, Michelle
LU
; Thorlacius, Henrik
LU
and Herwald, Heiko
LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cellular Microbiology
- volume
- 14
- pages
- 107 - 119
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000298061800010
- pmid:21951918
- scopus:83655163674
- pmid:21951918
- ISSN
- 1462-5814
- DOI
- 10.1111/j.1462-5822.2011.01705.x
- language
- English
- LU publication?
- yes
- id
- 54e3a5e8-7149-4018-8836-63905e803d98 (old id 2168362)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21951918?dopt=Abstract
- date added to LUP
- 2016-04-04 08:37:06
- date last changed
- 2022-01-29 03:43:01
@article{54e3a5e8-7149-4018-8836-63905e803d98,
abstract = {{Severe infectious diseases remain a major and life-threatening health problem. In serious cases a systemic activation of the coagulation cascade and hypovolemic shock are critical complications that are associated with high mortality rates. Here we report that blood mononuclear cells, stimulated with M1 protein of Streptococcus pyogenes or other bacterial virulence factors, produce not only pro-coagulant, but also pro-inflammatory microparticles (MPs). Our results also show that activation of the contact system on MPs contributes to these two effects. Phosphatidylserine (PS) plays an important role in these processes as its up-regulation on MPs allows an assembly and activation of the contact system. This in turn results in stabilization of the tissue factor-induced clot and a processing of high-molecular weight kininogen by plasma kallikrein followed by the release of bradykinin, a potent vascular mediator. Pro-coagulant monocyte-derived MPs were identified in plasma samples from septic patients and further analysis of MPs from these patients revealed that their pro-coagulant activity is dependent on the tissue factor- and contact system-driven pathway.}},
author = {{Oehmcke, Sonja and Mörgelin, Matthias and Malmström, Johan and Linder, Adam and Chew, Michelle and Thorlacius, Henrik and Herwald, Heiko}},
issn = {{1462-5814}},
language = {{eng}},
pages = {{107--119}},
publisher = {{Wiley-Blackwell}},
series = {{Cellular Microbiology}},
title = {{Stimulation of blood mononuclear cells with bacterial virulence factors leads to the release of pro-coagulant and pro-inflammatory microparticles.}},
url = {{http://dx.doi.org/10.1111/j.1462-5822.2011.01705.x}},
doi = {{10.1111/j.1462-5822.2011.01705.x}},
volume = {{14}},
year = {{2012}},
}