Carbamylation-Dependent Activation of T Cells: A Novel Mechanism in the Pathogenesis of Autoimmune Arthritis
(2010) In Journal of Immunology 184(12). p.6882-6890- Abstract
- The posttranslational modification of proteins has the potential to generate neoepitopes that may subsequently trigger immune responses. The carbamylation of lysine residues to form homocitrulline may be a key mechanism triggering inflammatory responses. We evaluated the role of carbamylation in triggering immune responses and report a new role for this process in the induction of arthritis. Immunization of mice with homocitrulline-containing peptides induced chemotaxis, T cell activation, and Ab production. The mice also developed erosive arthritis following intra-articular injection of peptides derived from homocitrulline and citrulline. Adoptive transfer of T and B cells from homocitrulline-immunized mice into normal recipients induced... (More)
- The posttranslational modification of proteins has the potential to generate neoepitopes that may subsequently trigger immune responses. The carbamylation of lysine residues to form homocitrulline may be a key mechanism triggering inflammatory responses. We evaluated the role of carbamylation in triggering immune responses and report a new role for this process in the induction of arthritis. Immunization of mice with homocitrulline-containing peptides induced chemotaxis, T cell activation, and Ab production. The mice also developed erosive arthritis following intra-articular injection of peptides derived from homocitrulline and citrulline. Adoptive transfer of T and B cells from homocitrulline-immunized mice into normal recipients induced arthritis, whereas systemic injection of homocitrulline-specific Abs or intra-articular injection of homocitrulline-Ab/citrulline-peptide mixture did not. Thus, the T cell response to homocitrulline-derived peptides, as well as the subsequent production of anti-homocitrulline Abs, is critical for the induction of autoimmune reactions against citrulline-derived peptides and provides a novel mechanism for the pathogenesis of arthritis. The Journal of Immunology, 2010, 184: 6882-6890. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1630972
- author
- Mydel, Piotr ; Wang, Zhenning LU ; Brisslert, Mikael ; Hellvard, Annelie ; Dahlberg, Leif LU ; Hazen, Stanley L. and Bokarewa, Maria
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Immunology
- volume
- 184
- issue
- 12
- pages
- 6882 - 6890
- publisher
- American Association of Immunologists
- external identifiers
-
- wos:000278516700036
- scopus:77953627756
- pmid:20488785
- ISSN
- 1550-6606
- DOI
- 10.4049/jimmunol.1000075
- language
- English
- LU publication?
- yes
- id
- 54fdfb3b-9f2d-4b2c-871c-975554bd9459 (old id 1630972)
- date added to LUP
- 2016-04-01 13:21:05
- date last changed
- 2022-04-21 21:08:36
@article{54fdfb3b-9f2d-4b2c-871c-975554bd9459, abstract = {{The posttranslational modification of proteins has the potential to generate neoepitopes that may subsequently trigger immune responses. The carbamylation of lysine residues to form homocitrulline may be a key mechanism triggering inflammatory responses. We evaluated the role of carbamylation in triggering immune responses and report a new role for this process in the induction of arthritis. Immunization of mice with homocitrulline-containing peptides induced chemotaxis, T cell activation, and Ab production. The mice also developed erosive arthritis following intra-articular injection of peptides derived from homocitrulline and citrulline. Adoptive transfer of T and B cells from homocitrulline-immunized mice into normal recipients induced arthritis, whereas systemic injection of homocitrulline-specific Abs or intra-articular injection of homocitrulline-Ab/citrulline-peptide mixture did not. Thus, the T cell response to homocitrulline-derived peptides, as well as the subsequent production of anti-homocitrulline Abs, is critical for the induction of autoimmune reactions against citrulline-derived peptides and provides a novel mechanism for the pathogenesis of arthritis. The Journal of Immunology, 2010, 184: 6882-6890.}}, author = {{Mydel, Piotr and Wang, Zhenning and Brisslert, Mikael and Hellvard, Annelie and Dahlberg, Leif and Hazen, Stanley L. and Bokarewa, Maria}}, issn = {{1550-6606}}, language = {{eng}}, number = {{12}}, pages = {{6882--6890}}, publisher = {{American Association of Immunologists}}, series = {{Journal of Immunology}}, title = {{Carbamylation-Dependent Activation of T Cells: A Novel Mechanism in the Pathogenesis of Autoimmune Arthritis}}, url = {{http://dx.doi.org/10.4049/jimmunol.1000075}}, doi = {{10.4049/jimmunol.1000075}}, volume = {{184}}, year = {{2010}}, }