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Maintenance of the E.coli dcm Methylation of the CMV Promoter, in Contrast to Hypomethylation of the Recognition Sequence of Transcription Factor NFkB in Transfected GBM Cells

Dock, H.; Sjögren, Hans Olov LU ; Salford, Leif LU ; Persson, Bertil R LU and Xue, Zhongtian LU (2016) In Cloning & Transgenesis 5(1).
Abstract
The human cytomegalovirus (CMV) immediate early promoter has been extensively used to drive target gene expression in transgenic mammalian cells. DNA methylation of the CMV promoter has been shown to be the reason for a reduced promoter activity and silencing of the target gene. We have established an in vitro model system, in which human brain cancer cells (glioblastoma multiforme, GBM) were transfected with pAdTrack-CMV-GFP plasmid, isolated from a dcm positive (dcm+) E. coli strain. We found that in two CCTGG sequences located at position from -304 to -300 nt and from -497 to -493 nt of the CMV promoter region, the internal C was methylated in all analyzed clones, i.e., the E. coli dcm methylation pattern is maintained in the CMV... (More)
The human cytomegalovirus (CMV) immediate early promoter has been extensively used to drive target gene expression in transgenic mammalian cells. DNA methylation of the CMV promoter has been shown to be the reason for a reduced promoter activity and silencing of the target gene. We have established an in vitro model system, in which human brain cancer cells (glioblastoma multiforme, GBM) were transfected with pAdTrack-CMV-GFP plasmid, isolated from a dcm positive (dcm+) E. coli strain. We found that in two CCTGG sequences located at position from -304 to -300 nt and from -497 to -493 nt of the CMV promoter region, the internal C was methylated in all analyzed clones, i.e., the E. coli dcm methylation pattern is maintained in the CMV promoter region after its integration into the human genome. In contrast, we found that the recognition sites for the transcription factor NFkB and certain other transcription factors in the enhancer region of the CMV promoter (from -107 to -270 nt) were hypomethylated. This might explain why the CMV promoter maintained an active mode, driving the GFP expression despite the demonstrated methylation of the CMV promoter. We noticed that the CCTGG sequence is also contained in the binding sequence motif of transcription factor NFkB. Hence we have comprehensively studied transcription factors through a database searching, and the responsive elements that contain dcm methylation sequences CCW(A/T)GG. A list of transcription factors and the corresponding regulated genes are presented.
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Contribution to journal
publication status
published
subject
in
Cloning & Transgenesis
volume
5
issue
1
publisher
OMICS
ISSN
2168-9849
DOI
10.4172/2168-9849.1000148
language
English
LU publication?
yes
id
550501e7-8190-437e-b69e-37bcb8857888
date added to LUP
2016-11-23 10:54:00
date last changed
2016-11-23 10:54:00
@article{550501e7-8190-437e-b69e-37bcb8857888,
  abstract     = {The human cytomegalovirus (CMV) immediate early promoter has been extensively used to drive target gene expression in transgenic mammalian cells. DNA methylation of the CMV promoter has been shown to be the reason for a reduced promoter activity and silencing of the target gene. We have established an in vitro model system, in which human brain cancer cells (glioblastoma multiforme, GBM) were transfected with pAdTrack-CMV-GFP plasmid, isolated from a dcm positive (dcm+) E. coli strain. We found that in two CCTGG sequences located at position from -304 to -300 nt and from -497 to -493 nt of the CMV promoter region, the internal C was methylated in all analyzed clones, i.e., the E. coli dcm methylation pattern is maintained in the CMV promoter region after its integration into the human genome. In contrast, we found that the recognition sites for the transcription factor NFkB and certain other transcription factors in the enhancer region of the CMV promoter (from -107 to -270 nt) were hypomethylated. This might explain why the CMV promoter maintained an active mode, driving the GFP expression despite the demonstrated methylation of the CMV promoter. We noticed that the CCTGG sequence is also contained in the binding sequence motif of transcription factor NFkB. Hence we have comprehensively studied transcription factors through a database searching, and the responsive elements that contain dcm methylation sequences CCW(A/T)GG. A list of transcription factors and the corresponding regulated genes are presented.<br/>},
  articleno    = {1000148},
  author       = {Dock, H. and Sjögren, Hans Olov and Salford, Leif and Persson, Bertil R and Xue, Zhongtian},
  issn         = {2168-9849},
  language     = {eng},
  number       = {1},
  publisher    = {OMICS},
  series       = {Cloning & Transgenesis },
  title        = {Maintenance of the E.coli dcm Methylation of the CMV Promoter, in Contrast to Hypomethylation of the Recognition Sequence of Transcription Factor NFkB in Transfected GBM Cells},
  url          = {http://dx.doi.org/10.4172/2168-9849.1000148},
  volume       = {5},
  year         = {2016},
}