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Toll-like receptor 3 agonist, polyinosinic-polycytidylic acid, upregulates carbonic anhydrase ii in human keratinocytes

Suri, Bani Kaur; Verma, Navin Kumar and Schmidtchen, Artur LU (2018) In Acta Dermato-Venereologica 98(8). p.762-765
Abstract

Carbonic anhydrases are ubiquitously expressed enzymes that reversibly hydrate carbon dioxide to bicar-bonate and protons. While the main function of carbonic anhydrases is to regulate pH and osmotic balance, their involvement in other physiological processes remains to be explored. This study analysed changes in mRNA and protein levels of carbonic anhydrase II in human primary keratinocytes treated with various toll-like receptor agonists and cytokines. A significant upregulation of carbonic anhydrase II at the mRNA and protein levels was observed upon treatment with polyinosinic-polycytidylic acid, a toll-like receptor 3 agonist. Furthermore, in agreement with the increased expression of carbonic anhydrase II in atopic dermatitis... (More)

Carbonic anhydrases are ubiquitously expressed enzymes that reversibly hydrate carbon dioxide to bicar-bonate and protons. While the main function of carbonic anhydrases is to regulate pH and osmotic balance, their involvement in other physiological processes remains to be explored. This study analysed changes in mRNA and protein levels of carbonic anhydrase II in human primary keratinocytes treated with various toll-like receptor agonists and cytokines. A significant upregulation of carbonic anhydrase II at the mRNA and protein levels was observed upon treatment with polyinosinic-polycytidylic acid, a toll-like receptor 3 agonist. Furthermore, in agreement with the increased expression of carbonic anhydrase II in atopic dermatitis skin, carbonic anhydrase II was upregulated by the Th2 cytokines interleukins-4 and-13. In conclusion, these results suggest a potential role of carbonic anhydrase II in Th2-dependent and toll-like receptor 3-induced pathways in inflammatory skin conditions.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Carbonic anhydrase II, Inflammation, Keratinocytes, Poly(I:C), Th2 cytokines, Tlr3
in
Acta Dermato-Venereologica
volume
98
issue
8
pages
4 pages
publisher
Medical Journals Limited
external identifiers
  • scopus:85055320213
ISSN
0001-5555
DOI
10.2340/00015555-2963
language
English
LU publication?
yes
id
55318fda-fd12-4718-a860-e2a7e1d9483b
date added to LUP
2018-11-22 09:32:31
date last changed
2019-02-20 11:37:07
@article{55318fda-fd12-4718-a860-e2a7e1d9483b,
  abstract     = {<p>Carbonic anhydrases are ubiquitously expressed enzymes that reversibly hydrate carbon dioxide to bicar-bonate and protons. While the main function of carbonic anhydrases is to regulate pH and osmotic balance, their involvement in other physiological processes remains to be explored. This study analysed changes in mRNA and protein levels of carbonic anhydrase II in human primary keratinocytes treated with various toll-like receptor agonists and cytokines. A significant upregulation of carbonic anhydrase II at the mRNA and protein levels was observed upon treatment with polyinosinic-polycytidylic acid, a toll-like receptor 3 agonist. Furthermore, in agreement with the increased expression of carbonic anhydrase II in atopic dermatitis skin, carbonic anhydrase II was upregulated by the Th2 cytokines interleukins-4 and-13. In conclusion, these results suggest a potential role of carbonic anhydrase II in Th2-dependent and toll-like receptor 3-induced pathways in inflammatory skin conditions.</p>},
  author       = {Suri, Bani Kaur and Verma, Navin Kumar and Schmidtchen, Artur},
  issn         = {0001-5555},
  keyword      = {Carbonic anhydrase II,Inflammation,Keratinocytes,Poly(I:C),Th2 cytokines,Tlr3},
  language     = {eng},
  number       = {8},
  pages        = {762--765},
  publisher    = {Medical Journals Limited},
  series       = {Acta Dermato-Venereologica},
  title        = {Toll-like receptor 3 agonist, polyinosinic-polycytidylic acid, upregulates carbonic anhydrase ii in human keratinocytes},
  url          = {http://dx.doi.org/10.2340/00015555-2963},
  volume       = {98},
  year         = {2018},
}