Acetic acid as a decontamination method for sink drains in a nosocomial outbreak of metallo-β-lactamase-producing Pseudomonas aeruginosa
(2016) In Journal of Hospital Infection 94(1). p.13-20- Abstract
Background Pseudomonas aeruginosa may colonize water systems via biofilm formation. In hospital environments, contaminated sinks have been associated with nosocomial transmission. Here we describe a prolonged outbreak of a metallo-β-lactamase-producing P. aeruginosa (Pae-MBL) associated with sink drains, and propose a previously unreported decontamination method with acetic acid. Aim To describe a nosocomial outbreak of Pae-MBL associated with hospital sink drains and to evaluate acetic acid as a decontamination method. Methods The outbreak was investigated by searching the microbiology database, microbiological sampling and strain typing. Antibacterial and antibiofilm properties of acetic acid were evaluated in vitro. Pae-MBL-positive... (More)
Background Pseudomonas aeruginosa may colonize water systems via biofilm formation. In hospital environments, contaminated sinks have been associated with nosocomial transmission. Here we describe a prolonged outbreak of a metallo-β-lactamase-producing P. aeruginosa (Pae-MBL) associated with sink drains, and propose a previously unreported decontamination method with acetic acid. Aim To describe a nosocomial outbreak of Pae-MBL associated with hospital sink drains and to evaluate acetic acid as a decontamination method. Methods The outbreak was investigated by searching the microbiology database, microbiological sampling and strain typing. Antibacterial and antibiofilm properties of acetic acid were evaluated in vitro. Pae-MBL-positive sinks were treated with 24% acetic acid once weekly and monitored with repeated cultures. Findings Fourteen patients with positive cultures for Pae-MBL were identified from 2008 to 2014. The patients had been admitted to three wards, where screening discovered Pae-MBL in 12 sink drains located in the patient bathrooms. Typing of clinical and sink drain isolates revealed identical or closely related strains. Pae-MBL biofilm was highly sensitive to acetic acid with a minimum biofilm eradication concentration of 0.75% (range: 0.19–1.5). Weekly treatment of colonized sink drains with acetic acid resulted in negative cultures and terminated transmission. Conclusion Acetic acid is highly effective against Pae-MBL biofilms, and may be used as a simple method to decontaminate sink drains and to prevent nosocomial transmission.
(Less)
- author
- Stjärne Aspelund, A. LU ; Sjöström, K. LU ; Olsson Liljequist, B. ; Mörgelin, M. LU ; Melander, E. LU and Påhlman, L. I. LU
- organization
- publishing date
- 2016-09-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Acetic acid, Biofilm, Decontamination, Metallo-β-lactamase producing Pseudomonas aeruginosa, Nosocomial outbreak, Sink drains
- in
- Journal of Hospital Infection
- volume
- 94
- issue
- 1
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:27346622
- wos:000381271000004
- scopus:84990837058
- ISSN
- 0195-6701
- DOI
- 10.1016/j.jhin.2016.05.009
- language
- English
- LU publication?
- yes
- id
- 55409577-67d7-4fdf-8b0c-609c52ccace2
- date added to LUP
- 2016-11-03 16:55:37
- date last changed
- 2025-01-12 14:21:24
@article{55409577-67d7-4fdf-8b0c-609c52ccace2,
abstract = {{<p>Background Pseudomonas aeruginosa may colonize water systems via biofilm formation. In hospital environments, contaminated sinks have been associated with nosocomial transmission. Here we describe a prolonged outbreak of a metallo-β-lactamase-producing P. aeruginosa (Pae-MBL) associated with sink drains, and propose a previously unreported decontamination method with acetic acid. Aim To describe a nosocomial outbreak of Pae-MBL associated with hospital sink drains and to evaluate acetic acid as a decontamination method. Methods The outbreak was investigated by searching the microbiology database, microbiological sampling and strain typing. Antibacterial and antibiofilm properties of acetic acid were evaluated in vitro. Pae-MBL-positive sinks were treated with 24% acetic acid once weekly and monitored with repeated cultures. Findings Fourteen patients with positive cultures for Pae-MBL were identified from 2008 to 2014. The patients had been admitted to three wards, where screening discovered Pae-MBL in 12 sink drains located in the patient bathrooms. Typing of clinical and sink drain isolates revealed identical or closely related strains. Pae-MBL biofilm was highly sensitive to acetic acid with a minimum biofilm eradication concentration of 0.75% (range: 0.19–1.5). Weekly treatment of colonized sink drains with acetic acid resulted in negative cultures and terminated transmission. Conclusion Acetic acid is highly effective against Pae-MBL biofilms, and may be used as a simple method to decontaminate sink drains and to prevent nosocomial transmission.</p>}},
author = {{Stjärne Aspelund, A. and Sjöström, K. and Olsson Liljequist, B. and Mörgelin, M. and Melander, E. and Påhlman, L. I.}},
issn = {{0195-6701}},
keywords = {{Acetic acid; Biofilm; Decontamination; Metallo-β-lactamase producing Pseudomonas aeruginosa; Nosocomial outbreak; Sink drains}},
language = {{eng}},
month = {{09}},
number = {{1}},
pages = {{13--20}},
publisher = {{Elsevier}},
series = {{Journal of Hospital Infection}},
title = {{Acetic acid as a decontamination method for sink drains in a nosocomial outbreak of metallo-β-lactamase-producing Pseudomonas aeruginosa}},
url = {{http://dx.doi.org/10.1016/j.jhin.2016.05.009}},
doi = {{10.1016/j.jhin.2016.05.009}},
volume = {{94}},
year = {{2016}},
}