Purification of a rotenone-insensitive NAD(P)H dehydrogenase from the inner surface of the inner membrane of red beetroot mitochondria
(1993) In Biochimica et Biophysica Acta - Bioenergetics 1141(1). p.107-110- Abstract
The soluble fraction of disrupted red beetroot mitochondria was resolved by anion-exchange chromatography. Three NADH-oxidising activities were found, including one duroquinone reductase oxidising both NADH and NADPH. This NAD(P)H-duroquinone reductase, which we assign as the internal rotenone-insensitive NAD(P)H dehydrogenase, was further purified by affinity chromatography into a 26 kDa polypeptide.
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- author
- Rasmusson, Allan G. LU ; Fredlund, Kenneth M. and Møller, Ian M.
- organization
- publishing date
- 1993-02-08
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- (Plant), Enzyme purification, Inner membrane, Mitochondrion, NAD(P)H dehydrogenase, Protein purification, Respiratory chain, Rotenone insensitivity
- in
- Biochimica et Biophysica Acta - Bioenergetics
- volume
- 1141
- issue
- 1
- pages
- 4 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0027471467
- ISSN
- 0005-2728
- DOI
- 10.1016/0005-2728(93)90196-M
- language
- English
- LU publication?
- yes
- id
- 555221c2-6ab7-4d5d-8ec8-3487b2ff21ba
- date added to LUP
- 2016-05-31 21:39:03
- date last changed
- 2024-01-04 07:46:26
@article{555221c2-6ab7-4d5d-8ec8-3487b2ff21ba, abstract = {{<p>The soluble fraction of disrupted red beetroot mitochondria was resolved by anion-exchange chromatography. Three NADH-oxidising activities were found, including one duroquinone reductase oxidising both NADH and NADPH. This NAD(P)H-duroquinone reductase, which we assign as the internal rotenone-insensitive NAD(P)H dehydrogenase, was further purified by affinity chromatography into a 26 kDa polypeptide.</p>}}, author = {{Rasmusson, Allan G. and Fredlund, Kenneth M. and Møller, Ian M.}}, issn = {{0005-2728}}, keywords = {{(Plant); Enzyme purification; Inner membrane; Mitochondrion; NAD(P)H dehydrogenase; Protein purification; Respiratory chain; Rotenone insensitivity}}, language = {{eng}}, month = {{02}}, number = {{1}}, pages = {{107--110}}, publisher = {{Elsevier}}, series = {{Biochimica et Biophysica Acta - Bioenergetics}}, title = {{Purification of a rotenone-insensitive NAD(P)H dehydrogenase from the inner surface of the inner membrane of red beetroot mitochondria}}, url = {{http://dx.doi.org/10.1016/0005-2728(93)90196-M}}, doi = {{10.1016/0005-2728(93)90196-M}}, volume = {{1141}}, year = {{1993}}, }