Two-step acoustofluidic cancer cell enrichment
(2024) Acoustofluidics 2024- Abstract
- We have previously proposed and evaluated acoustophoresis for the enrichment of circulating tumor cells (CTC) and clusters thereof from the blood of patients with prostate cancer [1]. In that protocol, the cells were fixed and preserved in paraformaldehyde before processing and were thus non-viable. To enable future enrichment of also viable CTCs from patients, we have in this study investigated the ability to discriminate cancer cell line cells from sub-groups of viable white blood cells (WBC). We found that acoustophoresis alone has poorer performance for viable cells than for fixed cells. Therefore, we added a second step of acoustic gradient separation wherein cells migrate into a medium of increasing acoustic impedance. The resulting... (More)
- We have previously proposed and evaluated acoustophoresis for the enrichment of circulating tumor cells (CTC) and clusters thereof from the blood of patients with prostate cancer [1]. In that protocol, the cells were fixed and preserved in paraformaldehyde before processing and were thus non-viable. To enable future enrichment of also viable CTCs from patients, we have in this study investigated the ability to discriminate cancer cell line cells from sub-groups of viable white blood cells (WBC). We found that acoustophoresis alone has poorer performance for viable cells than for fixed cells. Therefore, we added a second step of acoustic gradient separation wherein cells migrate into a medium of increasing acoustic impedance. The resulting separation results in >80% of cancer cells reaching the target outlet while 99.8% of all WBCs are removed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/559c5599-6e63-4349-8644-3e7529a4d1b2
- author
- Augustsson, Per LU
- organization
- publishing date
- 2024-08-14
- type
- Contribution to conference
- publication status
- published
- subject
- pages
- 2 pages
- conference name
- Acoustofluidics 2024
- conference location
- Uppsala, Sweden
- conference dates
- 2024-08-14 - 2024-08-16
- project
- Akustisk gradientfokusering för separation av biopartiklar
- High Throughput Microfluidic Cell Nanoparticle Handling by Molecular and Thermal Gradient Acoustic Focusing
- Akustisk flödescytometri för cancerdiagnostik
- language
- English
- LU publication?
- yes
- id
- 559c5599-6e63-4349-8644-3e7529a4d1b2
- date added to LUP
- 2024-12-02 14:14:50
- date last changed
- 2024-12-02 14:18:37
@misc{559c5599-6e63-4349-8644-3e7529a4d1b2,
abstract = {{We have previously proposed and evaluated acoustophoresis for the enrichment of circulating tumor cells (CTC) and clusters thereof from the blood of patients with prostate cancer [1]. In that protocol, the cells were fixed and preserved in paraformaldehyde before processing and were thus non-viable. To enable future enrichment of also viable CTCs from patients, we have in this study investigated the ability to discriminate cancer cell line cells from sub-groups of viable white blood cells (WBC). We found that acoustophoresis alone has poorer performance for viable cells than for fixed cells. Therefore, we added a second step of acoustic gradient separation wherein cells migrate into a medium of increasing acoustic impedance. The resulting separation results in >80% of cancer cells reaching the target outlet while 99.8% of all WBCs are removed.}},
author = {{Augustsson, Per}},
language = {{eng}},
month = {{08}},
title = {{Two-step acoustofluidic cancer cell enrichment}},
url = {{https://lup.lub.lu.se/search/files/201263305/Magnusson_Augustsson_Acoustofluidics_2024.pdf}},
year = {{2024}},
}