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CD105+CD90+CD13+ identifies a clonogenic subset of adventitial lung fibroblasts

Kadefors, Måns LU ; Rolandsson Enes, Sara LU orcid ; Åhrman, Emma LU ; Michaliková, Barbora LU ; Löfdahl, Anna LU ; Dellgren, Göran ; Scheding, Stefan LU and Westergren-Thorsson, Gunilla LU orcid (2021) In Scientific Reports 11(1).
Abstract

Mesenchymal cells are important components of specified niches in the lung, and can mediate a wide range of processes including tissue regeneration and repair. Dysregulation of these processes can lead to improper remodeling of tissue as observed in several lung diseases. The mesenchymal cells responsible remain poorly described, partially due to the heterogenic nature of the mesenchymal compartment and the absence of appropriate markers. Here, we describe that CD105+CD90+ mesenchymal cells can be divided into two populations based on their expression of CD13/aminopeptidase N (CD105+CD90+CD13− and CD105+CD90+CD13+). By prospective isolation using... (More)

Mesenchymal cells are important components of specified niches in the lung, and can mediate a wide range of processes including tissue regeneration and repair. Dysregulation of these processes can lead to improper remodeling of tissue as observed in several lung diseases. The mesenchymal cells responsible remain poorly described, partially due to the heterogenic nature of the mesenchymal compartment and the absence of appropriate markers. Here, we describe that CD105+CD90+ mesenchymal cells can be divided into two populations based on their expression of CD13/aminopeptidase N (CD105+CD90+CD13− and CD105+CD90+CD13+). By prospective isolation using FACS, we show that both these populations give rise to clonogenic fibroblast-like cells, but with an increased clonogenic and proliferative capacity of CD105+CD90+CD13+ cells. Transcriptomic and spatial analysis pinpoints an adventitial fibroblast subset as the origin of CD105+CD90+CD13+ clonogenic mesenchymal cells in human lung.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
11
issue
1
article number
24417
publisher
Nature Publishing Group
external identifiers
  • pmid:34952905
  • scopus:85121732402
ISSN
2045-2322
DOI
10.1038/s41598-021-03963-9
project
Characterization of lung-derived mesenchymal stromal cells
language
English
LU publication?
yes
id
55a3bd7a-ff86-4d69-a8d2-9b206a5dbc9f
date added to LUP
2022-02-21 14:52:37
date last changed
2024-06-13 10:53:21
@article{55a3bd7a-ff86-4d69-a8d2-9b206a5dbc9f,
  abstract     = {{<p>Mesenchymal cells are important components of specified niches in the lung, and can mediate a wide range of processes including tissue regeneration and repair. Dysregulation of these processes can lead to improper remodeling of tissue as observed in several lung diseases. The mesenchymal cells responsible remain poorly described, partially due to the heterogenic nature of the mesenchymal compartment and the absence of appropriate markers. Here, we describe that CD105<sup>+</sup>CD90<sup>+</sup> mesenchymal cells can be divided into two populations based on their expression of CD13/aminopeptidase N (CD105<sup>+</sup>CD90<sup>+</sup>CD13<sup>−</sup> and CD105<sup>+</sup>CD90<sup>+</sup>CD13<sup>+</sup>). By prospective isolation using FACS, we show that both these populations give rise to clonogenic fibroblast-like cells, but with an increased clonogenic and proliferative capacity of CD105<sup>+</sup>CD90<sup>+</sup>CD13<sup>+</sup> cells. Transcriptomic and spatial analysis pinpoints an adventitial fibroblast subset as the origin of CD105<sup>+</sup>CD90<sup>+</sup>CD13<sup>+</sup> clonogenic mesenchymal cells in human lung.</p>}},
  author       = {{Kadefors, Måns and Rolandsson Enes, Sara and Åhrman, Emma and Michaliková, Barbora and Löfdahl, Anna and Dellgren, Göran and Scheding, Stefan and Westergren-Thorsson, Gunilla}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{CD105<sup>+</sup>CD90<sup>+</sup>CD13<sup>+</sup> identifies a clonogenic subset of adventitial lung fibroblasts}},
  url          = {{http://dx.doi.org/10.1038/s41598-021-03963-9}},
  doi          = {{10.1038/s41598-021-03963-9}},
  volume       = {{11}},
  year         = {{2021}},
}