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Alcaligenes eutrophus cells containing hydrogenase, a coenzyme regenerating catalyst for NADH-dependent oxidoreductases

Andersson, Mats LU ; Otto, Ralf; Holmberg, Hans LU and Adlercreutz, Patrick LU (1997) In Biocatalysis and Biotransformation 15(4). p.281-296
Abstract

Alcaligenes eutrophus cells containing a soluble NAD-dependent hydrogenase was evaluated as a NADH regenerating catalyst. In the presence of hydrogen and permeabilized A. eutrophus cells, NADH regeneration was achieved, and reductions catalyzed by horse liver alcohol dehydrogenase and lactate dehydrogenase proceeded to 90-99% conversion. The operational stability of the hydrogenase was improved by the addition of reducing agents such as dithiothreitol and by immobilization of the cells in calcium alginate.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alcaligenes eutrophus, HLADH, Hydrogenase, LDH, NADH-regeneration
in
Biocatalysis and Biotransformation
volume
15
issue
4
pages
16 pages
publisher
Taylor & Francis
external identifiers
  • scopus:0031461928
ISSN
1024-2422
DOI
10.3109/10242429709003195
language
English
LU publication?
yes
id
56462a5f-95f4-4e7f-b043-c221bf087a26
date added to LUP
2019-06-20 16:16:18
date last changed
2019-09-21 02:18:11
@article{56462a5f-95f4-4e7f-b043-c221bf087a26,
  abstract     = {<p>Alcaligenes eutrophus cells containing a soluble NAD-dependent hydrogenase was evaluated as a NADH regenerating catalyst. In the presence of hydrogen and permeabilized A. eutrophus cells, NADH regeneration was achieved, and reductions catalyzed by horse liver alcohol dehydrogenase and lactate dehydrogenase proceeded to 90-99% conversion. The operational stability of the hydrogenase was improved by the addition of reducing agents such as dithiothreitol and by immobilization of the cells in calcium alginate.</p>},
  author       = {Andersson, Mats and Otto, Ralf and Holmberg, Hans and Adlercreutz, Patrick},
  issn         = {1024-2422},
  keyword      = {Alcaligenes eutrophus,HLADH,Hydrogenase,LDH,NADH-regeneration},
  language     = {eng},
  month        = {01},
  number       = {4},
  pages        = {281--296},
  publisher    = {Taylor & Francis},
  series       = {Biocatalysis and Biotransformation},
  title        = {Alcaligenes eutrophus cells containing hydrogenase, a coenzyme regenerating catalyst for NADH-dependent oxidoreductases},
  url          = {http://dx.doi.org/10.3109/10242429709003195},
  volume       = {15},
  year         = {1997},
}