Alcaligenes eutrophus cells containing hydrogenase, a coenzyme regenerating catalyst for NADH-dependent oxidoreductases
(1997) In Biocatalysis and Biotransformation 15(4). p.281-296- Abstract
Alcaligenes eutrophus cells containing a soluble NAD-dependent hydrogenase was evaluated as a NADH regenerating catalyst. In the presence of hydrogen and permeabilized A. eutrophus cells, NADH regeneration was achieved, and reductions catalyzed by horse liver alcohol dehydrogenase and lactate dehydrogenase proceeded to 90-99% conversion. The operational stability of the hydrogenase was improved by the addition of reducing agents such as dithiothreitol and by immobilization of the cells in calcium alginate.
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https://lup.lub.lu.se/record/56462a5f-95f4-4e7f-b043-c221bf087a26
- author
- Andersson, Mats LU ; Otto, Ralf ; Holmberg, Hans LU and Adlercreutz, Patrick LU
- organization
- publishing date
- 1997-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Alcaligenes eutrophus, HLADH, Hydrogenase, LDH, NADH-regeneration
- in
- Biocatalysis and Biotransformation
- volume
- 15
- issue
- 4
- pages
- 16 pages
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:0031461928
- ISSN
- 1024-2422
- DOI
- 10.3109/10242429709003195
- language
- English
- LU publication?
- yes
- id
- 56462a5f-95f4-4e7f-b043-c221bf087a26
- date added to LUP
- 2019-06-20 16:16:18
- date last changed
- 2022-01-31 22:13:26
@article{56462a5f-95f4-4e7f-b043-c221bf087a26, abstract = {{<p>Alcaligenes eutrophus cells containing a soluble NAD-dependent hydrogenase was evaluated as a NADH regenerating catalyst. In the presence of hydrogen and permeabilized A. eutrophus cells, NADH regeneration was achieved, and reductions catalyzed by horse liver alcohol dehydrogenase and lactate dehydrogenase proceeded to 90-99% conversion. The operational stability of the hydrogenase was improved by the addition of reducing agents such as dithiothreitol and by immobilization of the cells in calcium alginate.</p>}}, author = {{Andersson, Mats and Otto, Ralf and Holmberg, Hans and Adlercreutz, Patrick}}, issn = {{1024-2422}}, keywords = {{Alcaligenes eutrophus; HLADH; Hydrogenase; LDH; NADH-regeneration}}, language = {{eng}}, month = {{01}}, number = {{4}}, pages = {{281--296}}, publisher = {{Taylor & Francis}}, series = {{Biocatalysis and Biotransformation}}, title = {{Alcaligenes eutrophus cells containing hydrogenase, a coenzyme regenerating catalyst for NADH-dependent oxidoreductases}}, url = {{http://dx.doi.org/10.3109/10242429709003195}}, doi = {{10.3109/10242429709003195}}, volume = {{15}}, year = {{1997}}, }