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Increased parathyroid expression of klotho in uremic rats

Hofman-Bang, Jacob ; Martuseviciene, Giedre LU orcid ; Santini, Martin A ; Olgaard, Klaus and Lewin, Ewa (2010) In Kidney International 78(11). p.27-1119
Abstract

Klotho is a protein of significant importance for mineral homeostasis. It helps to increase parathyroid hormone (PTH) secretion and in the trafficking of Na+/K+-ATPase to the cell membrane; however, it is also a cofactor for fibroblast growth factor (FGF)-23 to interact with its receptor, FGFR1 IIIC, resulting in decreased PTH secretion. Studies on the regulation of parathyroid klotho expression in uremia have provided varying results. To help resolve this, we measured klotho expression in the parathyroid and its response to severe uremia, hyperphosphatemia, and calcitriol treatment in the 5/6 nephrectomy rat model of secondary hyperparathyroidism. Parathyroid klotho gene expression and protein were significantly increased in severely... (More)

Klotho is a protein of significant importance for mineral homeostasis. It helps to increase parathyroid hormone (PTH) secretion and in the trafficking of Na+/K+-ATPase to the cell membrane; however, it is also a cofactor for fibroblast growth factor (FGF)-23 to interact with its receptor, FGFR1 IIIC, resulting in decreased PTH secretion. Studies on the regulation of parathyroid klotho expression in uremia have provided varying results. To help resolve this, we measured klotho expression in the parathyroid and its response to severe uremia, hyperphosphatemia, and calcitriol treatment in the 5/6 nephrectomy rat model of secondary hyperparathyroidism. Parathyroid klotho gene expression and protein were significantly increased in severely uremic hyperphosphatemic rats, but not affected by moderate uremia and normal serum phosphorus. Calcitriol suppressed klotho gene and protein expression in severe secondary hyperparathyroidism, despite a further increase in plasma phosphate. Both FGFR1 IIIC and Na+/K+-ATPase gene expression were significantly elevated in severe secondary hyperparathyroidism. Parathyroid gland klotho expression and the plasma calcium ion concentration were inversely correlated. Thus, our study suggests that klotho may act as a positive regulator of PTH expression and secretion in secondary hyperparathyroidism.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Calcitriol/pharmacology, Calcium/blood, Disease Models, Animal, Fibroblast Growth Factors/metabolism, Glucuronidase/genetics, Hyperparathyroidism, Secondary/drug therapy, Hyperphosphatemia/etiology, Male, Nephrectomy, Parathyroid Glands/drug effects, Parathyroid Hormone/blood, Phosphorus/blood, RNA, Messenger/metabolism, Rats, Rats, Wistar, Receptor, Fibroblast Growth Factor, Type 1/genetics, Receptors, Calcitriol/genetics, Receptors, Calcium-Sensing/genetics, Sodium-Potassium-Exchanging ATPase/genetics, Up-Regulation, Uremia/drug therapy
in
Kidney International
volume
78
issue
11
pages
27 - 1119
publisher
Nature Publishing Group
external identifiers
  • scopus:78349310932
  • pmid:20631679
ISSN
1523-1755
DOI
10.1038/ki.2010.215
language
English
LU publication?
no
id
568b460d-86f5-4b3e-ad39-b52b71956a9f
date added to LUP
2019-10-24 19:50:15
date last changed
2024-04-02 20:23:27
@article{568b460d-86f5-4b3e-ad39-b52b71956a9f,
  abstract     = {{<p>Klotho is a protein of significant importance for mineral homeostasis. It helps to increase parathyroid hormone (PTH) secretion and in the trafficking of Na+/K+-ATPase to the cell membrane; however, it is also a cofactor for fibroblast growth factor (FGF)-23 to interact with its receptor, FGFR1 IIIC, resulting in decreased PTH secretion. Studies on the regulation of parathyroid klotho expression in uremia have provided varying results. To help resolve this, we measured klotho expression in the parathyroid and its response to severe uremia, hyperphosphatemia, and calcitriol treatment in the 5/6 nephrectomy rat model of secondary hyperparathyroidism. Parathyroid klotho gene expression and protein were significantly increased in severely uremic hyperphosphatemic rats, but not affected by moderate uremia and normal serum phosphorus. Calcitriol suppressed klotho gene and protein expression in severe secondary hyperparathyroidism, despite a further increase in plasma phosphate. Both FGFR1 IIIC and Na+/K+-ATPase gene expression were significantly elevated in severe secondary hyperparathyroidism. Parathyroid gland klotho expression and the plasma calcium ion concentration were inversely correlated. Thus, our study suggests that klotho may act as a positive regulator of PTH expression and secretion in secondary hyperparathyroidism.</p>}},
  author       = {{Hofman-Bang, Jacob and Martuseviciene, Giedre and Santini, Martin A and Olgaard, Klaus and Lewin, Ewa}},
  issn         = {{1523-1755}},
  keywords     = {{Animals; Calcitriol/pharmacology; Calcium/blood; Disease Models, Animal; Fibroblast Growth Factors/metabolism; Glucuronidase/genetics; Hyperparathyroidism, Secondary/drug therapy; Hyperphosphatemia/etiology; Male; Nephrectomy; Parathyroid Glands/drug effects; Parathyroid Hormone/blood; Phosphorus/blood; RNA, Messenger/metabolism; Rats; Rats, Wistar; Receptor, Fibroblast Growth Factor, Type 1/genetics; Receptors, Calcitriol/genetics; Receptors, Calcium-Sensing/genetics; Sodium-Potassium-Exchanging ATPase/genetics; Up-Regulation; Uremia/drug therapy}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{27--1119}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Kidney International}},
  title        = {{Increased parathyroid expression of klotho in uremic rats}},
  url          = {{http://dx.doi.org/10.1038/ki.2010.215}},
  doi          = {{10.1038/ki.2010.215}},
  volume       = {{78}},
  year         = {{2010}},
}