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Anti-amyloid compounds inhibit α-synuclein aggregation induced by protein misfolding cyclic amplification (PMCA)

Herva, Maria Eugenia; Zibaee, Shahin; Fraser, Graham; Barker, Roger A LU ; Goedert, Michel and Spillantini, Maria Grazia (2014) In Journal of Biological Chemistry 289(17). p.905-11897
Abstract

Filaments made of α-synuclein form the characteristic Lewy pathology in Parkinson and other diseases. The formation of α-synuclein filaments can be reproduced in vitro by incubation of recombinant protein, but the filament growth is very slow and highly variable and so unsuitable for fast high throughput anti-aggregation drug screening. To overcome this obstacle we have investigated whether the protein misfolding cyclic amplification (PMCA) technique, used for fast amplification of prion protein aggregates, could be adapted for growing α-synuclein aggregates and thus suitable for screening of drugs to affect α-synuclein aggregation for the treatment of the yet incurable α-synucleinopathies. Circular dichroism, electron microscopy, and... (More)

Filaments made of α-synuclein form the characteristic Lewy pathology in Parkinson and other diseases. The formation of α-synuclein filaments can be reproduced in vitro by incubation of recombinant protein, but the filament growth is very slow and highly variable and so unsuitable for fast high throughput anti-aggregation drug screening. To overcome this obstacle we have investigated whether the protein misfolding cyclic amplification (PMCA) technique, used for fast amplification of prion protein aggregates, could be adapted for growing α-synuclein aggregates and thus suitable for screening of drugs to affect α-synuclein aggregation for the treatment of the yet incurable α-synucleinopathies. Circular dichroism, electron microscopy, and native and SDS-polyacrylamide gels were used to demonstrate α-synuclein aggregate formation by PMCA, and the strain imprint of the α-synuclein fibrils was studied by proteinase K digestion. We also demonstrated that α-synuclein fibrils are able to seed new α-synuclein PMCA reactions and to enter and aggregate in cells in culture. In particular, we have generated a line of "chronically infected" cells, which transmit α-synuclein aggregates even after multiple passages. To evaluate the sensitivity of the PMCA system as an α-synuclein anti-aggregating drug screening assay a panel of 10 drugs was tested. Anti-amyloid compounds proved efficient in inhibiting α-synuclein fibril formation induced by PMCA. Our results show that α-synuclein PMCA is a fast and reproducible system that could be used as a high throughput screening method for finding new α-synuclein anti-aggregating compounds.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Amyloid, Cell Line, Humans, Protein Folding, Recombinant Proteins, Reproducibility of Results, alpha-Synuclein, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Biological Chemistry
volume
289
issue
17
pages
9 pages
publisher
ASBMB
external identifiers
  • scopus:84899422309
ISSN
1083-351X
DOI
10.1074/jbc.M113.542340
language
English
LU publication?
no
id
569d02ed-b13e-4072-8b52-f22b2032b97c
date added to LUP
2016-11-24 15:11:37
date last changed
2017-11-12 04:26:28
@article{569d02ed-b13e-4072-8b52-f22b2032b97c,
  abstract     = {<p>Filaments made of α-synuclein form the characteristic Lewy pathology in Parkinson and other diseases. The formation of α-synuclein filaments can be reproduced in vitro by incubation of recombinant protein, but the filament growth is very slow and highly variable and so unsuitable for fast high throughput anti-aggregation drug screening. To overcome this obstacle we have investigated whether the protein misfolding cyclic amplification (PMCA) technique, used for fast amplification of prion protein aggregates, could be adapted for growing α-synuclein aggregates and thus suitable for screening of drugs to affect α-synuclein aggregation for the treatment of the yet incurable α-synucleinopathies. Circular dichroism, electron microscopy, and native and SDS-polyacrylamide gels were used to demonstrate α-synuclein aggregate formation by PMCA, and the strain imprint of the α-synuclein fibrils was studied by proteinase K digestion. We also demonstrated that α-synuclein fibrils are able to seed new α-synuclein PMCA reactions and to enter and aggregate in cells in culture. In particular, we have generated a line of "chronically infected" cells, which transmit α-synuclein aggregates even after multiple passages. To evaluate the sensitivity of the PMCA system as an α-synuclein anti-aggregating drug screening assay a panel of 10 drugs was tested. Anti-amyloid compounds proved efficient in inhibiting α-synuclein fibril formation induced by PMCA. Our results show that α-synuclein PMCA is a fast and reproducible system that could be used as a high throughput screening method for finding new α-synuclein anti-aggregating compounds.</p>},
  author       = {Herva, Maria Eugenia and Zibaee, Shahin and Fraser, Graham and Barker, Roger A and Goedert, Michel and Spillantini, Maria Grazia},
  issn         = {1083-351X},
  keyword      = {Amyloid,Cell Line,Humans,Protein Folding,Recombinant Proteins,Reproducibility of Results,alpha-Synuclein,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {04},
  number       = {17},
  pages        = {905--11897},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Anti-amyloid compounds inhibit α-synuclein aggregation induced by protein misfolding cyclic amplification (PMCA)},
  url          = {http://dx.doi.org/10.1074/jbc.M113.542340},
  volume       = {289},
  year         = {2014},
}