Identification of New CTX Analogues in Fish from the Madeira and Selvagens Archipelagos by Neuro‐2a CBA and LC‐HRMS
(2022) In Marine Drugs 20(4).- Abstract
Ciguatera Poisoning (CP) is caused by consumption of fish or invertebrates contaminated with ciguatoxins (CTXs). Presently CP is a public concern in some temperate regions, such as Macaronesia (North‐Eastern Atlantic Ocean). Toxicity analysis was performed to characterize the fish species that can accumulate CTXs and improve understanding of the ciguatera risk in this area. For that, seventeen fish specimens comprising nine species were captured from coastal waters inMadeira and Selvagens Archipelagos. Toxicity was analysed by screening CTX‐like toxicity with the neuroblastoma cell‐based assay (neuro‐2a CBA). Afterwards, the four most toxic samples were analysed with liquid chromatography‐high resolution mass spectrometry (LC‐HRMS).... (More)
Ciguatera Poisoning (CP) is caused by consumption of fish or invertebrates contaminated with ciguatoxins (CTXs). Presently CP is a public concern in some temperate regions, such as Macaronesia (North‐Eastern Atlantic Ocean). Toxicity analysis was performed to characterize the fish species that can accumulate CTXs and improve understanding of the ciguatera risk in this area. For that, seventeen fish specimens comprising nine species were captured from coastal waters inMadeira and Selvagens Archipelagos. Toxicity was analysed by screening CTX‐like toxicity with the neuroblastoma cell‐based assay (neuro‐2a CBA). Afterwards, the four most toxic samples were analysed with liquid chromatography‐high resolution mass spectrometry (LC‐HRMS). Thirteen fish specimens presented CTX‐like toxicity in their liver, but only four of these in their muscle. The liver of one specimen of Muraena augusti presented the highest CTX‐like toxicity (0.270 ± 0.121 μg of CTX1B equiv∙kg−1). Moreover, CTX analogues were detected with LC‐HRMS, for M. augusti and Gymnothorax unicolor. The presence of three CTX analogues was identified: C‐CTX1, which had been previously described in the area; dihydro‐CTX2, which is reported in the area for the first time; a putative new CTX m/z 1127.6023 ([M+NH4]+) named as putative C‐CTX‐1109, and gambieric acid A.
(Less)
- author
- publishing date
- 2022-04
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- CBA, ciguatera, ciguatoxin, fish, LC‐HRMS, liver
- in
- Marine Drugs
- volume
- 20
- issue
- 4
- article number
- 236
- pages
- 19 pages
- publisher
- MDPI AG
- external identifiers
-
- pmid:35447910
- scopus:85128396537
- ISSN
- 1660-3397
- DOI
- 10.3390/md20040236
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
- id
- 56b49294-d90b-4ef7-bc9e-25ed8d73d6bd
- date added to LUP
- 2022-07-22 16:48:42
- date last changed
- 2024-09-20 01:10:43
@article{56b49294-d90b-4ef7-bc9e-25ed8d73d6bd, abstract = {{<p>Ciguatera Poisoning (CP) is caused by consumption of fish or invertebrates contaminated with ciguatoxins (CTXs). Presently CP is a public concern in some temperate regions, such as Macaronesia (North‐Eastern Atlantic Ocean). Toxicity analysis was performed to characterize the fish species that can accumulate CTXs and improve understanding of the ciguatera risk in this area. For that, seventeen fish specimens comprising nine species were captured from coastal waters inMadeira and Selvagens Archipelagos. Toxicity was analysed by screening CTX‐like toxicity with the neuroblastoma cell‐based assay (neuro‐2a CBA). Afterwards, the four most toxic samples were analysed with liquid chromatography‐high resolution mass spectrometry (LC‐HRMS). Thirteen fish specimens presented CTX‐like toxicity in their liver, but only four of these in their muscle. The liver of one specimen of <i>Muraena augusti</i> presented the highest CTX‐like toxicity (0.270 ± 0.121 μg of CTX1B equiv∙kg<sup>−1</sup>). Moreover, CTX analogues were detected with LC‐HRMS, for <i>M. augusti</i> and <i>Gymnothorax unicolor</i>. The presence of three CTX analogues was identified: C‐CTX1, which had been previously described in the area; dihydro‐CTX2, which is reported in the area for the first time; a putative new CTX <i>m</i>/<i>z</i> 1127.6023 ([M+NH4]<sup>+</sup>) named as putative C‐CTX‐1109, and gambieric acid A.</p>}}, author = {{Tudó, Àngels and Rambla-Alegre, Maria and Flores, Cintia and Sagristà, Núria and Aguayo, Paloma and Reverté, Laia and Campàs, Mònica and Gouveia, Neide and Santos, Carolina and Andree, Karl B. and Marques, Antonio and Caixach, Josep and Diogène, Jorge}}, issn = {{1660-3397}}, keywords = {{CBA; ciguatera; ciguatoxin; fish; LC‐HRMS; liver}}, language = {{eng}}, number = {{4}}, publisher = {{MDPI AG}}, series = {{Marine Drugs}}, title = {{Identification of New CTX Analogues in Fish from the Madeira and Selvagens Archipelagos by Neuro‐2a CBA and LC‐HRMS}}, url = {{http://dx.doi.org/10.3390/md20040236}}, doi = {{10.3390/md20040236}}, volume = {{20}}, year = {{2022}}, }