Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Identification of New CTX Analogues in Fish from the Madeira and Selvagens Archipelagos by Neuro‐2a CBA and LC‐HRMS

Tudó, Àngels LU ; Rambla-Alegre, Maria ; Flores, Cintia ; Sagristà, Núria ; Aguayo, Paloma ; Reverté, Laia ; Campàs, Mònica ; Gouveia, Neide ; Santos, Carolina and Andree, Karl B. , et al. (2022) In Marine Drugs 20(4).
Abstract

Ciguatera Poisoning (CP) is caused by consumption of fish or invertebrates contaminated with ciguatoxins (CTXs). Presently CP is a public concern in some temperate regions, such as Macaronesia (North‐Eastern Atlantic Ocean). Toxicity analysis was performed to characterize the fish species that can accumulate CTXs and improve understanding of the ciguatera risk in this area. For that, seventeen fish specimens comprising nine species were captured from coastal waters inMadeira and Selvagens Archipelagos. Toxicity was analysed by screening CTX‐like toxicity with the neuroblastoma cell‐based assay (neuro‐2a CBA). Afterwards, the four most toxic samples were analysed with liquid chromatography‐high resolution mass spectrometry (LC‐HRMS).... (More)

Ciguatera Poisoning (CP) is caused by consumption of fish or invertebrates contaminated with ciguatoxins (CTXs). Presently CP is a public concern in some temperate regions, such as Macaronesia (North‐Eastern Atlantic Ocean). Toxicity analysis was performed to characterize the fish species that can accumulate CTXs and improve understanding of the ciguatera risk in this area. For that, seventeen fish specimens comprising nine species were captured from coastal waters inMadeira and Selvagens Archipelagos. Toxicity was analysed by screening CTX‐like toxicity with the neuroblastoma cell‐based assay (neuro‐2a CBA). Afterwards, the four most toxic samples were analysed with liquid chromatography‐high resolution mass spectrometry (LC‐HRMS). Thirteen fish specimens presented CTX‐like toxicity in their liver, but only four of these in their muscle. The liver of one specimen of Muraena augusti presented the highest CTX‐like toxicity (0.270 ± 0.121 μg of CTX1B equiv∙kg−1). Moreover, CTX analogues were detected with LC‐HRMS, for M. augusti and Gymnothorax unicolor. The presence of three CTX analogues was identified: C‐CTX1, which had been previously described in the area; dihydro‐CTX2, which is reported in the area for the first time; a putative new CTX m/z 1127.6023 ([M+NH4]+) named as putative C‐CTX‐1109, and gambieric acid A.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; and (Less)
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CBA, ciguatera, ciguatoxin, fish, LC‐HRMS, liver
in
Marine Drugs
volume
20
issue
4
article number
236
pages
19 pages
publisher
MDPI AG
external identifiers
  • scopus:85128396537
  • pmid:35447910
ISSN
1660-3397
DOI
10.3390/md20040236
language
English
LU publication?
no
additional info
Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
id
56b49294-d90b-4ef7-bc9e-25ed8d73d6bd
date added to LUP
2022-07-22 16:48:42
date last changed
2024-06-13 16:03:06
@article{56b49294-d90b-4ef7-bc9e-25ed8d73d6bd,
  abstract     = {{<p>Ciguatera Poisoning (CP) is caused by consumption of fish or invertebrates contaminated with ciguatoxins (CTXs). Presently CP is a public concern in some temperate regions, such as Macaronesia (North‐Eastern Atlantic Ocean). Toxicity analysis was performed to characterize the fish species that can accumulate CTXs and improve understanding of the ciguatera risk in this area. For that, seventeen fish specimens comprising nine species were captured from coastal waters inMadeira and Selvagens Archipelagos. Toxicity was analysed by screening CTX‐like toxicity with the neuroblastoma cell‐based assay (neuro‐2a CBA). Afterwards, the four most toxic samples were analysed with liquid chromatography‐high resolution mass spectrometry (LC‐HRMS). Thirteen fish specimens presented CTX‐like toxicity in their liver, but only four of these in their muscle. The liver of one specimen of <i>Muraena augusti</i> presented the highest CTX‐like toxicity (0.270 ± 0.121 μg of CTX1B equiv∙kg<sup>−1</sup>). Moreover, CTX analogues were detected with LC‐HRMS, for <i>M. augusti</i> and <i>Gymnothorax unicolor</i>. The presence of three CTX analogues was identified: C‐CTX1, which had been previously described in the area; dihydro‐CTX2, which is reported in the area for the first time; a putative new CTX <i>m</i>/<i>z</i> 1127.6023 ([M+NH4]<sup>+</sup>) named as putative C‐CTX‐1109, and gambieric acid A.</p>}},
  author       = {{Tudó, Àngels and Rambla-Alegre, Maria and Flores, Cintia and Sagristà, Núria and Aguayo, Paloma and Reverté, Laia and Campàs, Mònica and Gouveia, Neide and Santos, Carolina and Andree, Karl B. and Marques, Antonio and Caixach, Josep and Diogène, Jorge}},
  issn         = {{1660-3397}},
  keywords     = {{CBA; ciguatera; ciguatoxin; fish; LC‐HRMS; liver}},
  language     = {{eng}},
  number       = {{4}},
  publisher    = {{MDPI AG}},
  series       = {{Marine Drugs}},
  title        = {{Identification of New CTX Analogues in Fish from the Madeira and Selvagens Archipelagos by Neuro‐2a CBA and LC‐HRMS}},
  url          = {{http://dx.doi.org/10.3390/md20040236}},
  doi          = {{10.3390/md20040236}},
  volume       = {{20}},
  year         = {{2022}},
}