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Flow cytometry immunophenotyping in integrated diagnostics of patients with newly diagnosed cytopenia : One tube 10-color 14-antibody screening panel and 3-tube extensive panel for detection of MDS-related features

Porwit, A. LU and Rajab, A. (2015) In International Journal of Laboratory Hematology 37(S1). p.133-143
Abstract

Acute leukemia, myelodysplastic syndromes (MDS), myeloproliferative neoplasms and lymphomas are the most prevalent diagnoses in adults presenting with new onset cytopenia. Here, we describe two 10-color panels of surface markers (screening and comprehensive panel) applied at the Flow Cytometry Laboratory, University Health Network, Toronto, ON, Canada. A 10-color flow cytometry is applied using the stain-lyse-wash sample preparation method. In patients with <10% blasts and no clear involvement by hematological malignancy based on cytomorphological evaluation of bone marrow (BM) smear, the recently published one-tube 10-color 14-antibody screening panel is applied. This panel allows detection of major B- and T-cell abnormalities,... (More)

Acute leukemia, myelodysplastic syndromes (MDS), myeloproliferative neoplasms and lymphomas are the most prevalent diagnoses in adults presenting with new onset cytopenia. Here, we describe two 10-color panels of surface markers (screening and comprehensive panel) applied at the Flow Cytometry Laboratory, University Health Network, Toronto, ON, Canada. A 10-color flow cytometry is applied using the stain-lyse-wash sample preparation method. In patients with <10% blasts and no clear involvement by hematological malignancy based on cytomorphological evaluation of bone marrow (BM) smear, the recently published one-tube 10-color 14-antibody screening panel is applied. This panel allows detection of major B- and T-cell abnormalities, enumeration of cells in blast region (CD45 dim), and gives insight into myeloid BM compartment, including calculation of four-parameter score for MDS-related abnormalities. In patients who present with ≥10 - <20% blasts in blood or BM smears, a comprehensive three-tube panel of surface markers is used up front. The analysis is focused on the detection of abnormal antigen expression patterns not seen in normal/reactive BM, according to the guidelines developed by International/European LeukemiaNet Working Group for Flow Cytometry in MDS. In patients with ≥20% blasts, an additional tube is added to allow the detection of cytoplasmic markers necessary to diagnose mixed phenotype acute leukemia.

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type
Contribution to journal
publication status
published
subject
keywords
Flow cytometry, Leukemia, Lymphoma, Myelodysplastic syndromes
in
International Journal of Laboratory Hematology
volume
37
issue
S1
pages
11 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:84929297827
  • pmid:25976971
ISSN
1751-5521
DOI
10.1111/ijlh.12368
language
English
LU publication?
no
id
5735b745-aed4-4523-8039-4531314a348c
date added to LUP
2019-05-22 09:44:52
date last changed
2024-05-28 12:15:24
@article{5735b745-aed4-4523-8039-4531314a348c,
  abstract     = {{<p>Acute leukemia, myelodysplastic syndromes (MDS), myeloproliferative neoplasms and lymphomas are the most prevalent diagnoses in adults presenting with new onset cytopenia. Here, we describe two 10-color panels of surface markers (screening and comprehensive panel) applied at the Flow Cytometry Laboratory, University Health Network, Toronto, ON, Canada. A 10-color flow cytometry is applied using the stain-lyse-wash sample preparation method. In patients with &lt;10% blasts and no clear involvement by hematological malignancy based on cytomorphological evaluation of bone marrow (BM) smear, the recently published one-tube 10-color 14-antibody screening panel is applied. This panel allows detection of major B- and T-cell abnormalities, enumeration of cells in blast region (CD45 dim), and gives insight into myeloid BM compartment, including calculation of four-parameter score for MDS-related abnormalities. In patients who present with ≥10 - &lt;20% blasts in blood or BM smears, a comprehensive three-tube panel of surface markers is used up front. The analysis is focused on the detection of abnormal antigen expression patterns not seen in normal/reactive BM, according to the guidelines developed by International/European LeukemiaNet Working Group for Flow Cytometry in MDS. In patients with ≥20% blasts, an additional tube is added to allow the detection of cytoplasmic markers necessary to diagnose mixed phenotype acute leukemia.</p>}},
  author       = {{Porwit, A. and Rajab, A.}},
  issn         = {{1751-5521}},
  keywords     = {{Flow cytometry; Leukemia; Lymphoma; Myelodysplastic syndromes}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{S1}},
  pages        = {{133--143}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{International Journal of Laboratory Hematology}},
  title        = {{Flow cytometry immunophenotyping in integrated diagnostics of patients with newly diagnosed cytopenia : One tube 10-color 14-antibody screening panel and 3-tube extensive panel for detection of MDS-related features}},
  url          = {{http://dx.doi.org/10.1111/ijlh.12368}},
  doi          = {{10.1111/ijlh.12368}},
  volume       = {{37}},
  year         = {{2015}},
}