Whole gene family expression and drought stress regulation of aquaporins
(2005) In Plant Molecular Biology 59(3). p.469-484- Abstract
- Since many aquaporins (AQPs) act as water channels, they are thought to play an important role in plant water relations. It is therefore of interest to study the expression patterns of AQP isoforms in order to further elucidate their involvement in plant water transport. We have monitored the expression patterns of all 35 Arabidopsis AQPs in leaves, roots and flowers by cDNA microarrays, specially designed for AQPs, and by quantitative real-time reverse transcriptase PCR (Q-RT-PCR). This showed that many AQPs are pre-dominantly expressed in either root or flower organs, whereas no AQP isoform seem to be leaf specific. Looking at the AQP subfamilies, most plasma membrane intrinsic proteins (PIPs) and some tonoplast intrinsic proteins (TIPs)... (More)
- Since many aquaporins (AQPs) act as water channels, they are thought to play an important role in plant water relations. It is therefore of interest to study the expression patterns of AQP isoforms in order to further elucidate their involvement in plant water transport. We have monitored the expression patterns of all 35 Arabidopsis AQPs in leaves, roots and flowers by cDNA microarrays, specially designed for AQPs, and by quantitative real-time reverse transcriptase PCR (Q-RT-PCR). This showed that many AQPs are pre-dominantly expressed in either root or flower organs, whereas no AQP isoform seem to be leaf specific. Looking at the AQP subfamilies, most plasma membrane intrinsic proteins (PIPs) and some tonoplast intrinsic proteins (TIPs) have a high level of expression, while NOD26-like proteins (NIPs) are present at a much lower level. In addition, we show that PIP transcripts are generally down-regulated upon gradual drought stress in leaves, with the exception of AtPIP1;4 and AtPIP2;5, which are up-regulated. AtPIP2;6 and AtSIP1;1 are constitutively expressed and not significantly affected by the drought stress. The transcriptional down-regulation of PIP genes upon drought stress could also be observed on the protein level. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/152447
- author
- Alexandersson, Erik LU ; Fraysse, Laure LU ; Sjövall Larsen, Sara LU ; Gustavsson, Sofia LU ; Agemark, Maria LU ; Karlsson, Maria LU ; Johanson, Urban LU and Kjellbom, Per LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Plant Molecular Biology
- volume
- 59
- issue
- 3
- pages
- 469 - 484
- publisher
- Springer
- external identifiers
-
- wos:000232694400006
- pmid:16235111
- scopus:26844456576
- pmid:16235111
- ISSN
- 1573-5028
- DOI
- 10.1007/s11103-005-0352-1
- language
- English
- LU publication?
- yes
- id
- 57df30ae-4e6d-4ac1-aaf0-4d657e40f327 (old id 152447)
- date added to LUP
- 2016-04-01 11:41:53
- date last changed
- 2022-04-20 20:25:04
@article{57df30ae-4e6d-4ac1-aaf0-4d657e40f327, abstract = {{Since many aquaporins (AQPs) act as water channels, they are thought to play an important role in plant water relations. It is therefore of interest to study the expression patterns of AQP isoforms in order to further elucidate their involvement in plant water transport. We have monitored the expression patterns of all 35 Arabidopsis AQPs in leaves, roots and flowers by cDNA microarrays, specially designed for AQPs, and by quantitative real-time reverse transcriptase PCR (Q-RT-PCR). This showed that many AQPs are pre-dominantly expressed in either root or flower organs, whereas no AQP isoform seem to be leaf specific. Looking at the AQP subfamilies, most plasma membrane intrinsic proteins (PIPs) and some tonoplast intrinsic proteins (TIPs) have a high level of expression, while NOD26-like proteins (NIPs) are present at a much lower level. In addition, we show that PIP transcripts are generally down-regulated upon gradual drought stress in leaves, with the exception of AtPIP1;4 and AtPIP2;5, which are up-regulated. AtPIP2;6 and AtSIP1;1 are constitutively expressed and not significantly affected by the drought stress. The transcriptional down-regulation of PIP genes upon drought stress could also be observed on the protein level.}}, author = {{Alexandersson, Erik and Fraysse, Laure and Sjövall Larsen, Sara and Gustavsson, Sofia and Agemark, Maria and Karlsson, Maria and Johanson, Urban and Kjellbom, Per}}, issn = {{1573-5028}}, language = {{eng}}, number = {{3}}, pages = {{469--484}}, publisher = {{Springer}}, series = {{Plant Molecular Biology}}, title = {{Whole gene family expression and drought stress regulation of aquaporins}}, url = {{http://dx.doi.org/10.1007/s11103-005-0352-1}}, doi = {{10.1007/s11103-005-0352-1}}, volume = {{59}}, year = {{2005}}, }