Advanced

Macro-/nanoporous silicon as a support for high-performance protein microarrays

Ressine, Anton LU ; Ekström, Simon LU ; Marko-Varga, György LU and Laurell, Thomas LU (2003) In Analytical Chemistry 75(24). p.6968-6974
Abstract
The present work demonstrates the possibilities of using macroporous silicon as a substrate for highly sensitive protein chip applications. The formation of 3D porous silicon structures was performed by electrochemical dissolution of monocrystalline silicon. The fabricated macroporous silicon network has a rigid spongelike structure showing high uniformity and mechanical stability. The microfluidic properties of the substrates were found to be essential for a good bioassay performance. Small spot area, good spot reproducibility, and homogeneous spot profiles were demonstrated on the substrates for immobilized aRIgG. Water contact angles were measured on the porous surface and compared to that of planar silicon, silanized glass, and... (More)
The present work demonstrates the possibilities of using macroporous silicon as a substrate for highly sensitive protein chip applications. The formation of 3D porous silicon structures was performed by electrochemical dissolution of monocrystalline silicon. The fabricated macroporous silicon network has a rigid spongelike structure showing high uniformity and mechanical stability. The microfluidic properties of the substrates were found to be essential for a good bioassay performance. Small spot area, good spot reproducibility, and homogeneous spot profiles were demonstrated on the substrates for immobilized aRIgG. Water contact angles were measured on the porous surface and compared to that of planar silicon, silanized glass, and ordinary microscope glass slides. The effect of the porous surface on the performance of a model IgG-binding immunoassay is presented. aRIgG was microdispensed onto the chip surface forming a microarray of spots with high affinity for the target analyte. The dispensing was performed using an in-house-developed piezoelectric flow-through dispenser. Each spot was formed by a single droplet (100 pL) at each position. The macroporous silicon allowed a high-density microarraying with spot densities up to 4400 spots/cm(2) in human plasma samples without cross-talk and consumption of only 0.6 pmol of antibodies/1-cm(2) array. Antigen levels down to 70 pM were detected. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
75
issue
24
pages
6968 - 6974
publisher
The American Chemical Society
external identifiers
  • wos:000187283000028
  • pmid:14670060
  • scopus:0348014618
ISSN
1520-6882
DOI
10.1021/ac034425q
language
English
LU publication?
yes
id
58717de8-ce7c-494e-9000-10108733e57a (old id 292516)
date added to LUP
2007-09-20 15:33:17
date last changed
2018-05-13 03:33:07
@article{58717de8-ce7c-494e-9000-10108733e57a,
  abstract     = {The present work demonstrates the possibilities of using macroporous silicon as a substrate for highly sensitive protein chip applications. The formation of 3D porous silicon structures was performed by electrochemical dissolution of monocrystalline silicon. The fabricated macroporous silicon network has a rigid spongelike structure showing high uniformity and mechanical stability. The microfluidic properties of the substrates were found to be essential for a good bioassay performance. Small spot area, good spot reproducibility, and homogeneous spot profiles were demonstrated on the substrates for immobilized aRIgG. Water contact angles were measured on the porous surface and compared to that of planar silicon, silanized glass, and ordinary microscope glass slides. The effect of the porous surface on the performance of a model IgG-binding immunoassay is presented. aRIgG was microdispensed onto the chip surface forming a microarray of spots with high affinity for the target analyte. The dispensing was performed using an in-house-developed piezoelectric flow-through dispenser. Each spot was formed by a single droplet (100 pL) at each position. The macroporous silicon allowed a high-density microarraying with spot densities up to 4400 spots/cm(2) in human plasma samples without cross-talk and consumption of only 0.6 pmol of antibodies/1-cm(2) array. Antigen levels down to 70 pM were detected.},
  author       = {Ressine, Anton and Ekström, Simon and Marko-Varga, György and Laurell, Thomas},
  issn         = {1520-6882},
  language     = {eng},
  number       = {24},
  pages        = {6968--6974},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Macro-/nanoporous silicon as a support for high-performance protein microarrays},
  url          = {http://dx.doi.org/10.1021/ac034425q},
  volume       = {75},
  year         = {2003},
}