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In vitro and in vivo biofilm formation by pathogenic streptococci

Chao, Yashuan LU ; Bergenfelz, Caroline LU and Håkansson, Anders P. LU (2017) In Methods in Molecular Biology 1535. p.285-299
Abstract

This manuscript presents novel approaches to grow and evaluate Streptococcal biofilm formation using the human respiratory pathogen Streptococcus pneumoniae (the pneumococcus) as the main model organism on biological surfaces in vitro and in vivo. Most biofilm models are based on growth on abiotic surfaces, which is relevant for many pathogens whose growth on surfaces or medical devices is a major cause of disease transmission and infections, especially in hospital environments. However, most infections with commensal organisms require biofilm formation on biological surfaces in the host at the site of colonization or infection. In vitro model systems incorporating biological components from the host and taking into account the host... (More)

This manuscript presents novel approaches to grow and evaluate Streptococcal biofilm formation using the human respiratory pathogen Streptococcus pneumoniae (the pneumococcus) as the main model organism on biological surfaces in vitro and in vivo. Most biofilm models are based on growth on abiotic surfaces, which is relevant for many pathogens whose growth on surfaces or medical devices is a major cause of disease transmission and infections, especially in hospital environments. However, most infections with commensal organisms require biofilm formation on biological surfaces in the host at the site of colonization or infection. In vitro model systems incorporating biological components from the host and taking into account the host environment of the infectious site are not well described. In a series of publications, we have shown that S. pneumoniae form complex biofilms in the nasopharynx of mice and have devised methodology to evaluate the biofilm structure and function in this environment. We have also been able to recapitulate this biofilm phenotype in vitro by incorporating crucial factors associated with the host environment. Although the protocols presented in this manuscript are focused on S. pneumoniae, the same methodology can and has been used for other Streptococcal species that form biofilms on mucosal surfaces.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Biofilm, Colonization, Epithelium, Mucosa, Nasopharynx, Respiratory tract, Streptococci, Streptococcus pneumoniae, Virulence
in
Methods in Molecular Biology
volume
1535
pages
15 pages
publisher
Humana Press
external identifiers
  • scopus:85005939784
ISSN
10643745
DOI
10.1007/978-1-4939-6673-8_19
language
English
LU publication?
yes
id
5875afa7-6468-4741-88b2-bd174329b39d
date added to LUP
2017-03-15 16:00:21
date last changed
2017-03-16 03:00:03
@inbook{5875afa7-6468-4741-88b2-bd174329b39d,
  abstract     = {<p>This manuscript presents novel approaches to grow and evaluate Streptococcal biofilm formation using the human respiratory pathogen Streptococcus pneumoniae (the pneumococcus) as the main model organism on biological surfaces in vitro and in vivo. Most biofilm models are based on growth on abiotic surfaces, which is relevant for many pathogens whose growth on surfaces or medical devices is a major cause of disease transmission and infections, especially in hospital environments. However, most infections with commensal organisms require biofilm formation on biological surfaces in the host at the site of colonization or infection. In vitro model systems incorporating biological components from the host and taking into account the host environment of the infectious site are not well described. In a series of publications, we have shown that S. pneumoniae form complex biofilms in the nasopharynx of mice and have devised methodology to evaluate the biofilm structure and function in this environment. We have also been able to recapitulate this biofilm phenotype in vitro by incorporating crucial factors associated with the host environment. Although the protocols presented in this manuscript are focused on S. pneumoniae, the same methodology can and has been used for other Streptococcal species that form biofilms on mucosal surfaces.</p>},
  author       = {Chao, Yashuan and Bergenfelz, Caroline and Håkansson, Anders P.},
  issn         = {10643745},
  keyword      = {Biofilm,Colonization,Epithelium,Mucosa,Nasopharynx,Respiratory tract,Streptococci,Streptococcus pneumoniae,Virulence},
  language     = {eng},
  pages        = {285--299},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {In vitro and in vivo biofilm formation by pathogenic streptococci},
  url          = {http://dx.doi.org/10.1007/978-1-4939-6673-8_19},
  volume       = {1535},
  year         = {2017},
}