In vitro and in vivo biofilm formation by pathogenic streptococci
Chao, Yashuan LU ; Bergenfelz, Caroline LU
and Håkansson, Anders P.
LU
(2017)
In Methods in Molecular Biology
1535.
p.285-299
- Abstract
This manuscript presents novel approaches to grow and evaluate Streptococcal biofilm formation using the human respiratory pathogen Streptococcus pneumoniae (the pneumococcus) as the main model organism on biological surfaces in vitro and in vivo. Most biofilm models are based on growth on abiotic surfaces, which is relevant for many pathogens whose growth on surfaces or medical devices is a major cause of disease transmission and infections, especially in hospital environments. However, most infections with commensal organisms require biofilm formation on biological surfaces in the host at the site of colonization or infection. In vitro model systems incorporating biological components from the host and taking into account the host... (More)
This manuscript presents novel approaches to grow and evaluate Streptococcal biofilm formation using the human respiratory pathogen Streptococcus pneumoniae (the pneumococcus) as the main model organism on biological surfaces in vitro and in vivo. Most biofilm models are based on growth on abiotic surfaces, which is relevant for many pathogens whose growth on surfaces or medical devices is a major cause of disease transmission and infections, especially in hospital environments. However, most infections with commensal organisms require biofilm formation on biological surfaces in the host at the site of colonization or infection. In vitro model systems incorporating biological components from the host and taking into account the host environment of the infectious site are not well described. In a series of publications, we have shown that S. pneumoniae form complex biofilms in the nasopharynx of mice and have devised methodology to evaluate the biofilm structure and function in this environment. We have also been able to recapitulate this biofilm phenotype in vitro by incorporating crucial factors associated with the host environment. Although the protocols presented in this manuscript are focused on S. pneumoniae, the same methodology can and has been used for other Streptococcal species that form biofilms on mucosal surfaces.
(Less)
- author
- Chao, Yashuan
LU
; Bergenfelz, Caroline
LU
and Håkansson, Anders P.
LU
- organization
- publishing date
- 2017
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Biofilm, Colonization, Epithelium, Mucosa, Nasopharynx, Respiratory tract, Streptococci, Streptococcus pneumoniae, Virulence
- host publication
- Methods in Molecular Biology
- series title
- Methods in Molecular Biology
- volume
- 1535
- pages
- 15 pages
- publisher
- Humana Press
- external identifiers
-
- pmid:27914087
- scopus:85005939784
- ISSN
- 10643745
- DOI
- 10.1007/978-1-4939-6673-8_19
- language
- English
- LU publication?
- yes
- id
- 5875afa7-6468-4741-88b2-bd174329b39d
- date added to LUP
- 2017-03-15 16:00:21
- date last changed
- 2025-02-03 12:30:00
@inbook{5875afa7-6468-4741-88b2-bd174329b39d,
abstract = {{<p>This manuscript presents novel approaches to grow and evaluate Streptococcal biofilm formation using the human respiratory pathogen Streptococcus pneumoniae (the pneumococcus) as the main model organism on biological surfaces in vitro and in vivo. Most biofilm models are based on growth on abiotic surfaces, which is relevant for many pathogens whose growth on surfaces or medical devices is a major cause of disease transmission and infections, especially in hospital environments. However, most infections with commensal organisms require biofilm formation on biological surfaces in the host at the site of colonization or infection. In vitro model systems incorporating biological components from the host and taking into account the host environment of the infectious site are not well described. In a series of publications, we have shown that S. pneumoniae form complex biofilms in the nasopharynx of mice and have devised methodology to evaluate the biofilm structure and function in this environment. We have also been able to recapitulate this biofilm phenotype in vitro by incorporating crucial factors associated with the host environment. Although the protocols presented in this manuscript are focused on S. pneumoniae, the same methodology can and has been used for other Streptococcal species that form biofilms on mucosal surfaces.</p>}},
author = {{Chao, Yashuan and Bergenfelz, Caroline and Håkansson, Anders P.}},
booktitle = {{Methods in Molecular Biology}},
issn = {{10643745}},
keywords = {{Biofilm; Colonization; Epithelium; Mucosa; Nasopharynx; Respiratory tract; Streptococci; Streptococcus pneumoniae; Virulence}},
language = {{eng}},
pages = {{285--299}},
publisher = {{Humana Press}},
series = {{Methods in Molecular Biology}},
title = {{In vitro and in vivo biofilm formation by pathogenic streptococci}},
url = {{http://dx.doi.org/10.1007/978-1-4939-6673-8_19}},
doi = {{10.1007/978-1-4939-6673-8_19}},
volume = {{1535}},
year = {{2017}},
}