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Hydroxyapatite granule/carrier composites promote new bone formation in cortical defects

Liljensten, E L; Attaelmanan, A G; Larsson, C; Ljusberg-Wahren, H; Danielsen, N LU ; Hirsch, J M and Thomsen, P. (2000) In Clinical Implant Dentistry and Related Research 2(1). p.9-50
Abstract

BACKGROUND: A great deal of interest has been focused on finding substitutes for autogenous bone grafts. Among the most interesting materials are different calcium phosphate compositions (e.g., hydroxyapatite [HA]), due to their biocompatible properties in hard and soft tissue.

PURPOSE: The bone response to porous ceramic HA granules in combination with two lipid and one polysaccharide carrier was evaluated in an experimental bone defect model in rabbits.

MATERIALS AND METHODS: Circular defects (Ø 4 mm) were made in both tibias of 32 rabbits. The 64 defects were divided into four groups. Group A was augmented with a composite of HA granules and a phospholipid-diacetyl-glycerol carrier, group B with HA granules and a... (More)

BACKGROUND: A great deal of interest has been focused on finding substitutes for autogenous bone grafts. Among the most interesting materials are different calcium phosphate compositions (e.g., hydroxyapatite [HA]), due to their biocompatible properties in hard and soft tissue.

PURPOSE: The bone response to porous ceramic HA granules in combination with two lipid and one polysaccharide carrier was evaluated in an experimental bone defect model in rabbits.

MATERIALS AND METHODS: Circular defects (Ø 4 mm) were made in both tibias of 32 rabbits. The 64 defects were divided into four groups. Group A was augmented with a composite of HA granules and a phospholipid-diacetyl-glycerol carrier, group B with HA granules and a phospholipid carrier, group C received HA granules and a sodium hyaluronan carrier, and group D served as control. The animals were killed after 6 weeks and ground sections were evaluated using light microscopic morphometry. X-ray microfluorescence (XRF) was applied in order to evaluate the suitability of this method to examine bone-biomaterial interfaces. Calcium distribution was studied using x-ray fluorescence line scans at selected interface regions of two sections in group B.

RESULTS: The HA/phospholipid composites were easier to shape and handle than the HA/hyaluronan composite. Group A had 36% newly formed bone area within the defect. Groups B and C showed significantly more newly formed bone within the defect (47% and 49%, respectively) compared to the control group (31%). The XRF analysis revealed that the amount of calcium in the newly formed bone was similar to that observed for the HA granules and slightly lower when compared to the mature, lamellar bone.

CONCLUSIONS: Synchrotron radiation may be a new, suitable technique to study the interface between bone and biomaterials with regard to mineral content. The results suggest that HA granule/lipid and HA granule/hyaluronan composites have interesting properties as bone-substitute materials.

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publication status
published
subject
keywords
Animals, Biocompatible Materials, Bone Diseases, Bone Substitutes, Calcium, Ceramics, Connective Tissue, Diacetyl, Drug Carriers, Durapatite, Electron Probe Microanalysis, Female, Glycerol, Hyaluronic Acid, Male, Osteogenesis, Phosphatidylcholines, Phospholipids, Rabbits, Spectrometry, X-Ray Emission, Statistics, Nonparametric, Surface Properties, Tibia, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
in
Clinical Implant Dentistry and Related Research
volume
2
issue
1
pages
10 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0034570405
ISSN
1523-0899
DOI
10.1111/j.1708-8208.2000.tb00106.x
language
English
LU publication?
yes
id
58e4bd50-9aa0-40c4-b3c8-1320d5d32510
date added to LUP
2017-10-13 13:02:10
date last changed
2017-10-29 05:07:24
@article{58e4bd50-9aa0-40c4-b3c8-1320d5d32510,
  abstract     = {<p>BACKGROUND: A great deal of interest has been focused on finding substitutes for autogenous bone grafts. Among the most interesting materials are different calcium phosphate compositions (e.g., hydroxyapatite [HA]), due to their biocompatible properties in hard and soft tissue.</p><p>PURPOSE: The bone response to porous ceramic HA granules in combination with two lipid and one polysaccharide carrier was evaluated in an experimental bone defect model in rabbits.</p><p>MATERIALS AND METHODS: Circular defects (Ø 4 mm) were made in both tibias of 32 rabbits. The 64 defects were divided into four groups. Group A was augmented with a composite of HA granules and a phospholipid-diacetyl-glycerol carrier, group B with HA granules and a phospholipid carrier, group C received HA granules and a sodium hyaluronan carrier, and group D served as control. The animals were killed after 6 weeks and ground sections were evaluated using light microscopic morphometry. X-ray microfluorescence (XRF) was applied in order to evaluate the suitability of this method to examine bone-biomaterial interfaces. Calcium distribution was studied using x-ray fluorescence line scans at selected interface regions of two sections in group B.</p><p>RESULTS: The HA/phospholipid composites were easier to shape and handle than the HA/hyaluronan composite. Group A had 36% newly formed bone area within the defect. Groups B and C showed significantly more newly formed bone within the defect (47% and 49%, respectively) compared to the control group (31%). The XRF analysis revealed that the amount of calcium in the newly formed bone was similar to that observed for the HA granules and slightly lower when compared to the mature, lamellar bone.</p><p>CONCLUSIONS: Synchrotron radiation may be a new, suitable technique to study the interface between bone and biomaterials with regard to mineral content. The results suggest that HA granule/lipid and HA granule/hyaluronan composites have interesting properties as bone-substitute materials.</p>},
  author       = {Liljensten, E L and Attaelmanan, A G and Larsson, C and Ljusberg-Wahren, H and Danielsen, N and Hirsch, J M and Thomsen, P.},
  issn         = {1523-0899},
  keyword      = {Animals,Biocompatible Materials,Bone Diseases,Bone Substitutes,Calcium,Ceramics,Connective Tissue,Diacetyl,Drug Carriers,Durapatite,Electron Probe Microanalysis,Female,Glycerol,Hyaluronic Acid,Male,Osteogenesis,Phosphatidylcholines,Phospholipids,Rabbits,Spectrometry, X-Ray Emission,Statistics, Nonparametric,Surface Properties,Tibia,Comparative Study,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {1},
  pages        = {9--50},
  publisher    = {Wiley-Blackwell},
  series       = {Clinical Implant Dentistry and Related Research},
  title        = {Hydroxyapatite granule/carrier composites promote new bone formation in cortical defects},
  url          = {http://dx.doi.org/10.1111/j.1708-8208.2000.tb00106.x},
  volume       = {2},
  year         = {2000},
}