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Nitric oxide exposure diverts neural stem cell fate from neurogenesis towards astrogliogenesis

Covacu, Ruxandra; Danilov, Alexandre LU ; Rasmussen, Bo Sonnich; Hallen, Katarina; Moe, Morten C; Lobell, Anna; Johansson, Clas B; Svensson, Mikael A; Olsson, Tomas and Brundin, Lou (2006) In Stem Cells 24(12). p.2792-2800
Abstract
Regeneration of cells in the central nervous system is a process that might be affected during neurological disease and trauma. Because nitric oxide (NO) and its derivatives are powerful mediators in the inflammatory cascade, we have investigated the effects of pathophysiological concentrations of NO on neurogenesis, gliogenesis, and the expression of proneural genes in primary adult neural stem cell cultures. After exposure to NO, neurogenesis was downregulated, and this corresponded to decreased expression of the proneural gene neurogenin-2 and beta-III-tubulin. The decreased ability to generate neurons was also found to be transmitted to the progeny of the cells. NO exposure was instead beneficial for astroglial differentiation, which... (More)
Regeneration of cells in the central nervous system is a process that might be affected during neurological disease and trauma. Because nitric oxide (NO) and its derivatives are powerful mediators in the inflammatory cascade, we have investigated the effects of pathophysiological concentrations of NO on neurogenesis, gliogenesis, and the expression of proneural genes in primary adult neural stem cell cultures. After exposure to NO, neurogenesis was downregulated, and this corresponded to decreased expression of the proneural gene neurogenin-2 and beta-III-tubulin. The decreased ability to generate neurons was also found to be transmitted to the progeny of the cells. NO exposure was instead beneficial for astroglial differentiation, which was confirmed by increased activation of the Janus tyrosine kinase/signal transducer and activator of transcription transduction pathway. Our findings reveal a new role for NO during neuroinflammatory conditions, whereby its proastroglial fate-determining effect on neural stem cells might directly influence the neuroregenerative process. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Autoimmune disease, Nitric oxide, Neural stem cell, Neural differentiation, Glial differentiation
in
Stem Cells
volume
24
issue
12
pages
2792 - 2800
publisher
AlphaMed Press
external identifiers
  • pmid:16916924
  • scopus:33846027059
ISSN
1549-4918
DOI
10.1634/stemcells.2005-0640
language
English
LU publication?
yes
id
5a0c3ba4-25cb-4053-a80c-47d38785de8c (old id 1137319)
date added to LUP
2008-06-03 10:22:58
date last changed
2019-07-30 03:05:32
@article{5a0c3ba4-25cb-4053-a80c-47d38785de8c,
  abstract     = {Regeneration of cells in the central nervous system is a process that might be affected during neurological disease and trauma. Because nitric oxide (NO) and its derivatives are powerful mediators in the inflammatory cascade, we have investigated the effects of pathophysiological concentrations of NO on neurogenesis, gliogenesis, and the expression of proneural genes in primary adult neural stem cell cultures. After exposure to NO, neurogenesis was downregulated, and this corresponded to decreased expression of the proneural gene neurogenin-2 and beta-III-tubulin. The decreased ability to generate neurons was also found to be transmitted to the progeny of the cells. NO exposure was instead beneficial for astroglial differentiation, which was confirmed by increased activation of the Janus tyrosine kinase/signal transducer and activator of transcription transduction pathway. Our findings reveal a new role for NO during neuroinflammatory conditions, whereby its proastroglial fate-determining effect on neural stem cells might directly influence the neuroregenerative process.},
  author       = {Covacu, Ruxandra and Danilov, Alexandre and Rasmussen, Bo Sonnich and Hallen, Katarina and Moe, Morten C and Lobell, Anna and Johansson, Clas B and Svensson, Mikael A and Olsson, Tomas and Brundin, Lou},
  issn         = {1549-4918},
  keyword      = {Autoimmune disease,Nitric oxide,Neural stem cell,Neural differentiation,Glial differentiation},
  language     = {eng},
  number       = {12},
  pages        = {2792--2800},
  publisher    = {AlphaMed Press},
  series       = {Stem Cells},
  title        = {Nitric oxide exposure diverts neural stem cell fate from neurogenesis towards astrogliogenesis},
  url          = {http://dx.doi.org/10.1634/stemcells.2005-0640},
  volume       = {24},
  year         = {2006},
}