SEPARATION OF CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT
(2021) 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 p.1201-1202- Abstract
Differences in morphologies of bacteria and bacteria clusters are known to influence their pathogenicity. However, it is difficult to separate cells and cell clusters based on morphology using standard cell biological methods, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of clusters of group A streptococci, based on cluster size and morphology, using Deterministic Lateral Displacement (DLD). In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5ab9285e-7efa-4888-be8e-ad37f7b663ed
- author
- Akbari, Elham LU ; Beech, Jason P. LU ; Nordenfelt, Pontus LU and Tegenfeldt, Jonas LU
- organization
- publishing date
- 2021
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- bacteria clusters, Deterministic Lateral Displacement, group A streptococci, Label-free cell separation
- host publication
- MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
- conference location
- Palm Springs, Virtual, United States
- conference dates
- 2021-10-10 - 2021-10-14
- external identifiers
-
- scopus:85136922901
- ISBN
- 9781733419031
- language
- English
- LU publication?
- yes
- additional info
- Funding Information: This work was carried out within NanoLund at Lund University, device processing was conducted in Lund NanoLab, and bacteria samples were prepared by Nordenfelt group within Department of Clinical Sciences at Lund University. It was funded by the Swedish Research Council (grant number 2019-04102). Publisher Copyright: © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
- id
- 5ab9285e-7efa-4888-be8e-ad37f7b663ed
- date added to LUP
- 2022-11-08 09:55:03
- date last changed
- 2023-10-23 06:27:40
@inproceedings{5ab9285e-7efa-4888-be8e-ad37f7b663ed, abstract = {{<p>Differences in morphologies of bacteria and bacteria clusters are known to influence their pathogenicity. However, it is difficult to separate cells and cell clusters based on morphology using standard cell biological methods, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of clusters of group A streptococci, based on cluster size and morphology, using Deterministic Lateral Displacement (DLD). In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.</p>}}, author = {{Akbari, Elham and Beech, Jason P. and Nordenfelt, Pontus and Tegenfeldt, Jonas}}, booktitle = {{MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences}}, isbn = {{9781733419031}}, keywords = {{bacteria clusters; Deterministic Lateral Displacement; group A streptococci; Label-free cell separation}}, language = {{eng}}, pages = {{1201--1202}}, publisher = {{Chemical and Biological Microsystems Society}}, title = {{SEPARATION OF CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT}}, year = {{2021}}, }