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SEPARATION OF CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT

Akbari, Elham LU ; Beech, Jason P. LU ; Nordenfelt, Pontus LU orcid and Tegenfeldt, Jonas LU orcid (2021) 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 p.1201-1202
Abstract

Differences in morphologies of bacteria and bacteria clusters are known to influence their pathogenicity. However, it is difficult to separate cells and cell clusters based on morphology using standard cell biological methods, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of clusters of group A streptococci, based on cluster size and morphology, using Deterministic Lateral Displacement (DLD). In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.

Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
bacteria clusters, Deterministic Lateral Displacement, group A streptococci, Label-free cell separation
host publication
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
conference location
Palm Springs, Virtual, United States
conference dates
2021-10-10 - 2021-10-14
external identifiers
  • scopus:85136922901
ISBN
9781733419031
language
English
LU publication?
yes
additional info
Funding Information: This work was carried out within NanoLund at Lund University, device processing was conducted in Lund NanoLab, and bacteria samples were prepared by Nordenfelt group within Department of Clinical Sciences at Lund University. It was funded by the Swedish Research Council (grant number 2019-04102). Publisher Copyright: © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
id
5ab9285e-7efa-4888-be8e-ad37f7b663ed
date added to LUP
2022-11-08 09:55:03
date last changed
2023-10-23 06:27:40
@inproceedings{5ab9285e-7efa-4888-be8e-ad37f7b663ed,
  abstract     = {{<p>Differences in morphologies of bacteria and bacteria clusters are known to influence their pathogenicity. However, it is difficult to separate cells and cell clusters based on morphology using standard cell biological methods, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of clusters of group A streptococci, based on cluster size and morphology, using Deterministic Lateral Displacement (DLD). In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.</p>}},
  author       = {{Akbari, Elham and Beech, Jason P. and Nordenfelt, Pontus and Tegenfeldt, Jonas}},
  booktitle    = {{MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences}},
  isbn         = {{9781733419031}},
  keywords     = {{bacteria clusters; Deterministic Lateral Displacement; group A streptococci; Label-free cell separation}},
  language     = {{eng}},
  pages        = {{1201--1202}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  title        = {{SEPARATION OF CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT}},
  year         = {{2021}},
}