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Silicon microstructures for high-speed and high-sensitivity protein identifications

Laurell, Thomas LU ; Nilsson, J. and Marko-Varga, György LU (2001) In Journal of Chromatography. B, Biomedical Sciences and Applications 752(2). p.217-232
Abstract
Silicon microtechnology has been used to develop a microstructure toolbox in order to enable high accuracy protein identification. During the last 2 years we developed and applied monocrystalline silicon structures and established new automated protein analysis platforms, The development of a high throughput protein platform is presented where fully automated protein identifications are performed. It includes the reduction and alkylation of the protein sample in a standard 96- or 384-well plate format prior to injection of 1 mul samples into the continuous flow based microtechnology platform. The processed sample is transferred to a microchip nanovial array target using piezoelectric microdispensing. identification is made by MALDI-TOF MS... (More)
Silicon microtechnology has been used to develop a microstructure toolbox in order to enable high accuracy protein identification. During the last 2 years we developed and applied monocrystalline silicon structures and established new automated protein analysis platforms, The development of a high throughput protein platform is presented where fully automated protein identifications are performed. It includes the reduction and alkylation of the protein sample in a standard 96- or 384-well plate format prior to injection of 1 mul samples into the continuous flow based microtechnology platform. The processed sample is transferred to a microchip nanovial array target using piezoelectric microdispensing. identification is made by MALDI-TOF MS and a database search. After the initial sample reduction and alkylation period of 50 min the platform can digest and process protein samples at a speed of 100 samples in 210 min. An optional configuration of the platform, operating the dispenser in the 'static mode', enables on-target enrichment of low abundant proteins and peptides e.g. from 2DE samples. This makes detection at the low attomole level possible. (C) 2001 Elsevier Science B.V. All rights reserved. (Less)
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type
Contribution to journal
publication status
published
subject
keywords
silicon microstructures, proteins, FLIGHT MASS-SPECTROMETRY, FLOW-THROUGH DISPENSER, SAMPLE PREPARATION, POROUS SILICON, MATRIX, MALDI
in
Journal of Chromatography. B, Biomedical Sciences and Applications
volume
752
issue
2
pages
217 - 232
publisher
Elsevier
external identifiers
  • wos:000167347000004
  • scopus:0035836056
ISSN
1387-2273
DOI
10.1016/s0378-4347(00)00358-3
language
English
LU publication?
yes
id
5af31fe7-2756-482d-aef2-f62f56de6e49 (old id 2376386)
date added to LUP
2016-04-01 16:25:11
date last changed
2022-01-28 19:34:05
@article{5af31fe7-2756-482d-aef2-f62f56de6e49,
  abstract     = {{Silicon microtechnology has been used to develop a microstructure toolbox in order to enable high accuracy protein identification. During the last 2 years we developed and applied monocrystalline silicon structures and established new automated protein analysis platforms, The development of a high throughput protein platform is presented where fully automated protein identifications are performed. It includes the reduction and alkylation of the protein sample in a standard 96- or 384-well plate format prior to injection of 1 mul samples into the continuous flow based microtechnology platform. The processed sample is transferred to a microchip nanovial array target using piezoelectric microdispensing. identification is made by MALDI-TOF MS and a database search. After the initial sample reduction and alkylation period of 50 min the platform can digest and process protein samples at a speed of 100 samples in 210 min. An optional configuration of the platform, operating the dispenser in the 'static mode', enables on-target enrichment of low abundant proteins and peptides e.g. from 2DE samples. This makes detection at the low attomole level possible. (C) 2001 Elsevier Science B.V. All rights reserved.}},
  author       = {{Laurell, Thomas and Nilsson, J. and Marko-Varga, György}},
  issn         = {{1387-2273}},
  keywords     = {{silicon microstructures; proteins; FLIGHT MASS-SPECTROMETRY; FLOW-THROUGH DISPENSER; SAMPLE PREPARATION; POROUS SILICON; MATRIX; MALDI}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{217--232}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography. B, Biomedical Sciences and Applications}},
  title        = {{Silicon microstructures for high-speed and high-sensitivity protein identifications}},
  url          = {{http://dx.doi.org/10.1016/s0378-4347(00)00358-3}},
  doi          = {{10.1016/s0378-4347(00)00358-3}},
  volume       = {{752}},
  year         = {{2001}},
}