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Efficient methodology for the extraction and analysis of lipids from porcine pulmonary artery by supercritical fluid chromatography coupled to mass spectrometry

Gil-Ramirez, Alicia LU ; Al-Hamimi, Said LU ; Rosmark, Oskar LU ; Hallgren, Oskar LU ; Larsson-Callerfelt, Anna Karin LU and Rodríguez-Meizoso, Irene LU (2019) In Journal of Chromatography A
Abstract

Pulmonary artery grafts are needed as cardiovascular bioprosthetics. For successful tissue recellularization after transplantation, lipids have to be removed from the donor artery. Developing a selective process to remove lipids without damaging the extracellular matrix greatly depends on knowing the amount and type of lipid compounds in the specific tissue. Here we present an efficient methodology for the study of lipids present in porcine pulmonary arteries. The performance of six extraction methods to recover lipids from artery was evaluated. For this purpose, a supercritical fluid chromatography method coupled to quadrupole time-of-flight mass spectrometry detection (UHPSFC/QTOF-MS) was adapted. The method enabled separation of... (More)

Pulmonary artery grafts are needed as cardiovascular bioprosthetics. For successful tissue recellularization after transplantation, lipids have to be removed from the donor artery. Developing a selective process to remove lipids without damaging the extracellular matrix greatly depends on knowing the amount and type of lipid compounds in the specific tissue. Here we present an efficient methodology for the study of lipids present in porcine pulmonary arteries. The performance of six extraction methods to recover lipids from artery was evaluated. For this purpose, a supercritical fluid chromatography method coupled to quadrupole time-of-flight mass spectrometry detection (UHPSFC/QTOF-MS) was adapted. The method enabled separation of lipids of a wide range of polarity according to lipid class in less than 7 min. One dichloromethane-based extraction method was shown to be the most efficient one for the recovery of lipids from pulmonary artery. However, one MTBE-based extraction method was able to show the highest fatty acid extraction yields (to the expense of longer extraction times). Lipids were relative quantified according to class, and the major species within each class were identified. Triacylglycerols and glycerophospholipids were the most abundant classes, followed by sphingomyelins, monoacylglycerols and fatty acyls. The matrix effect exerted no interference on the analytical method, except for some few combinations of extraction method and lipid class. These results are of relevance for lipidomic studies from solid tissue, in particular for studies on pulmonary and cardiovascular diseases. Finally, our work sets the basis for the further development of a selective processes to remove lipids from pulmonary artery without damaging the tissue prior to transplantation.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Extraction, Lipids, Mass spectrometry, Pulmonary arteries, Supercritical fluid chromatography
in
Journal of Chromatography A
publisher
Elsevier
external identifiers
  • scopus:85060648344
ISSN
0021-9673
DOI
10.1016/j.chroma.2019.01.064
language
English
LU publication?
yes
id
5b8d36fc-5430-43d1-bd85-c159d2e6a24d
date added to LUP
2019-02-06 15:13:52
date last changed
2019-03-05 04:31:47
@article{5b8d36fc-5430-43d1-bd85-c159d2e6a24d,
  abstract     = {<p>Pulmonary artery grafts are needed as cardiovascular bioprosthetics. For successful tissue recellularization after transplantation, lipids have to be removed from the donor artery. Developing a selective process to remove lipids without damaging the extracellular matrix greatly depends on knowing the amount and type of lipid compounds in the specific tissue. Here we present an efficient methodology for the study of lipids present in porcine pulmonary arteries. The performance of six extraction methods to recover lipids from artery was evaluated. For this purpose, a supercritical fluid chromatography method coupled to quadrupole time-of-flight mass spectrometry detection (UHPSFC/QTOF-MS) was adapted. The method enabled separation of lipids of a wide range of polarity according to lipid class in less than 7 min. One dichloromethane-based extraction method was shown to be the most efficient one for the recovery of lipids from pulmonary artery. However, one MTBE-based extraction method was able to show the highest fatty acid extraction yields (to the expense of longer extraction times). Lipids were relative quantified according to class, and the major species within each class were identified. Triacylglycerols and glycerophospholipids were the most abundant classes, followed by sphingomyelins, monoacylglycerols and fatty acyls. The matrix effect exerted no interference on the analytical method, except for some few combinations of extraction method and lipid class. These results are of relevance for lipidomic studies from solid tissue, in particular for studies on pulmonary and cardiovascular diseases. Finally, our work sets the basis for the further development of a selective processes to remove lipids from pulmonary artery without damaging the tissue prior to transplantation.</p>},
  author       = {Gil-Ramirez, Alicia and Al-Hamimi, Said and Rosmark, Oskar and Hallgren, Oskar and Larsson-Callerfelt, Anna Karin and Rodríguez-Meizoso, Irene},
  issn         = {0021-9673},
  keyword      = {Extraction,Lipids,Mass spectrometry,Pulmonary arteries,Supercritical fluid chromatography},
  language     = {eng},
  month        = {01},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Efficient methodology for the extraction and analysis of lipids from porcine pulmonary artery by supercritical fluid chromatography coupled to mass spectrometry},
  url          = {http://dx.doi.org/10.1016/j.chroma.2019.01.064},
  year         = {2019},
}