Kinetics of ethanol production by recombinant Escherichia coli KO11
(1995) In Biotechnology and Bioengineering 45(4). p.356-365- Abstract
- The fermentation kinetics for separate as well as simultaneous glucose and xylose fermentation with recombinant ethanologenic Escherichia coli KO11 are presented. Glucose and xylose were consumed simultaneously and exhibited mutual inhibition. The glucose exhibited 15 times stronger inhibition in xylose fermentation than vice versa. The fermentation of condensate from steam-pretreated willow (Salix) was investigated. The kinetics were studied in detoxified as well as in nondetoxified condensate. The fermentation of the condensate followed two phases: First the glucose and some of the pentoses (xylose in addition to small amounts of arabinose) were fermented simultaneously, and then the remaining part of the pentoses were fermented. The... (More)
- The fermentation kinetics for separate as well as simultaneous glucose and xylose fermentation with recombinant ethanologenic Escherichia coli KO11 are presented. Glucose and xylose were consumed simultaneously and exhibited mutual inhibition. The glucose exhibited 15 times stronger inhibition in xylose fermentation than vice versa. The fermentation of condensate from steam-pretreated willow (Salix) was investigated. The kinetics were studied in detoxified as well as in nondetoxified condensate. The fermentation of the condensate followed two phases: First the glucose and some of the pentoses (xylose in addition to small amounts of arabinose) were fermented simultaneously, and then the remaining part of the pentoses were fermented. The rate of the first phase was independent of the detoxification method used, whereas the rate of the second phase was found to be strongly dependent. When the condensate was detoxified with overliming in combination with sulfite, which was the best detoxification method investigated, the sugars in the condensate, 9 g/L, were fermented in 11 h. The same fermentation took 150 h in nondetoxified condensate, The experimental data were used to develop an empirical model, describing the batch fermentation of recombinant E. coli KO11 in the condensate. The model is based on Monod kinetics including substrate and product inhibition and the sum of the inhibition exerted by the rest of the inhibitors, lumped together. (C) 1995 John Wiley and Sons, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3911553
- author
- Olsson, L ; Hahn-Hägerdal, Bärbel LU and Zacchi, Guido LU
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- LIGNOCELLULOSIC HYDROLYSATE, KINETIC MODEL, ESCHERICHIA COLI K011, ETHANOL PRODUCTION, FERMENTATION, MIXED SUGAR
- in
- Biotechnology and Bioengineering
- volume
- 45
- issue
- 4
- pages
- 356 - 365
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:A1995QJ67000009
- scopus:0029239619
- ISSN
- 1097-0290
- DOI
- 10.1002/bit.260450410
- language
- English
- LU publication?
- yes
- id
- 5beb75ff-dfe9-4315-aa21-90cb4283fc1b (old id 3911553)
- date added to LUP
- 2016-04-01 11:42:36
- date last changed
- 2023-09-01 04:14:21
@article{5beb75ff-dfe9-4315-aa21-90cb4283fc1b, abstract = {{The fermentation kinetics for separate as well as simultaneous glucose and xylose fermentation with recombinant ethanologenic Escherichia coli KO11 are presented. Glucose and xylose were consumed simultaneously and exhibited mutual inhibition. The glucose exhibited 15 times stronger inhibition in xylose fermentation than vice versa. The fermentation of condensate from steam-pretreated willow (Salix) was investigated. The kinetics were studied in detoxified as well as in nondetoxified condensate. The fermentation of the condensate followed two phases: First the glucose and some of the pentoses (xylose in addition to small amounts of arabinose) were fermented simultaneously, and then the remaining part of the pentoses were fermented. The rate of the first phase was independent of the detoxification method used, whereas the rate of the second phase was found to be strongly dependent. When the condensate was detoxified with overliming in combination with sulfite, which was the best detoxification method investigated, the sugars in the condensate, 9 g/L, were fermented in 11 h. The same fermentation took 150 h in nondetoxified condensate, The experimental data were used to develop an empirical model, describing the batch fermentation of recombinant E. coli KO11 in the condensate. The model is based on Monod kinetics including substrate and product inhibition and the sum of the inhibition exerted by the rest of the inhibitors, lumped together. (C) 1995 John Wiley and Sons, Inc.}}, author = {{Olsson, L and Hahn-Hägerdal, Bärbel and Zacchi, Guido}}, issn = {{1097-0290}}, keywords = {{LIGNOCELLULOSIC HYDROLYSATE; KINETIC MODEL; ESCHERICHIA COLI K011; ETHANOL PRODUCTION; FERMENTATION; MIXED SUGAR}}, language = {{eng}}, number = {{4}}, pages = {{356--365}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Biotechnology and Bioengineering}}, title = {{Kinetics of ethanol production by recombinant Escherichia coli KO11}}, url = {{http://dx.doi.org/10.1002/bit.260450410}}, doi = {{10.1002/bit.260450410}}, volume = {{45}}, year = {{1995}}, }