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Enhanced Ribosome and tRNA Contents in Escherichia coli Expressing a Truncated Vitreoscilla Hemoglobin Mutant Analyzed by Flow Field-Flow Fractionation

Andersson, Charlotte LU ; Arfvidsson, Cecilia LU ; Kallio, P T ; Wahlund, Karl-Gustav LU and Bülow, Leif LU (2003) In Biotechnology Letters 25(18). p.1499-1504
Abstract
The ribosome and tRNA levels of Escherichia coli cells, transformed with a native or mutated Vitreoscilla hemoglobin genes (vhb), were investigated using asymmetrical flow field-flow fractionation (AFFFF). Mutagenesis of vhb by error-prone PCR was carried out to alter the growth behavior of microaerobically cultivated native VHb-expressing E. coli. A VHb mutant, pVMT1, was identified, which was able to reach a remarkably high final A600 of 15, the value of which being 160% higher than that of a VHb control carrying pVHb8 (A600 5.8). AFFFF revealed that cells expressing mutant vhbs showed up to a doubling in the number of active 70S ribosomes cell-1, an almost 3-fold increase in the number of tRNAs cell-1, and up to a 26% increase in the... (More)
The ribosome and tRNA levels of Escherichia coli cells, transformed with a native or mutated Vitreoscilla hemoglobin genes (vhb), were investigated using asymmetrical flow field-flow fractionation (AFFFF). Mutagenesis of vhb by error-prone PCR was carried out to alter the growth behavior of microaerobically cultivated native VHb-expressing E. coli. A VHb mutant, pVMT1, was identified, which was able to reach a remarkably high final A600 of 15, the value of which being 160% higher than that of a VHb control carrying pVHb8 (A600 5.8). AFFFF revealed that cells expressing mutant vhbs showed up to a doubling in the number of active 70S ribosomes cell-1, an almost 3-fold increase in the number of tRNAs cell-1, and up to a 26% increase in the mass fraction of active 70S ribosomes. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Letters
volume
25
issue
18
pages
1499 - 1504
publisher
Springer Science and Business Media B.V.
external identifiers
  • pmid:14571972
  • wos:000184957400003
  • scopus:0141789646
ISSN
1573-6776
DOI
10.1023/A:1025475703006
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Pure and Applied Biochemistry (LTH) (011001005)
id
5c230f1c-dd3d-46b8-ada3-76e93b1afa16 (old id 128589)
date added to LUP
2016-04-01 16:55:05
date last changed
2025-10-14 11:24:48
@article{5c230f1c-dd3d-46b8-ada3-76e93b1afa16,
  abstract     = {{The ribosome and tRNA levels of Escherichia coli cells, transformed with a native or mutated Vitreoscilla hemoglobin genes (vhb), were investigated using asymmetrical flow field-flow fractionation (AFFFF). Mutagenesis of vhb by error-prone PCR was carried out to alter the growth behavior of microaerobically cultivated native VHb-expressing E. coli. A VHb mutant, pVMT1, was identified, which was able to reach a remarkably high final A600 of 15, the value of which being 160% higher than that of a VHb control carrying pVHb8 (A600 5.8). AFFFF revealed that cells expressing mutant vhbs showed up to a doubling in the number of active 70S ribosomes cell-1, an almost 3-fold increase in the number of tRNAs cell-1, and up to a 26% increase in the mass fraction of active 70S ribosomes.}},
  author       = {{Andersson, Charlotte and Arfvidsson, Cecilia and Kallio, P T and Wahlund, Karl-Gustav and Bülow, Leif}},
  issn         = {{1573-6776}},
  language     = {{eng}},
  number       = {{18}},
  pages        = {{1499--1504}},
  publisher    = {{Springer Science and Business Media B.V.}},
  series       = {{Biotechnology Letters}},
  title        = {{Enhanced Ribosome and tRNA Contents in Escherichia coli Expressing a Truncated Vitreoscilla Hemoglobin Mutant Analyzed by Flow Field-Flow Fractionation}},
  url          = {{http://dx.doi.org/10.1023/A:1025475703006}},
  doi          = {{10.1023/A:1025475703006}},
  volume       = {{25}},
  year         = {{2003}},
}