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DNA sample cleanup using deterministic lateral displacement

Kim, Rebekah LU ; Kühnlein, Alexandra; Smith, Darren A.; Ho, Bao Dang LU ; Grunwald, Assaf; Beech, Jason P. LU ; Neely, Robert K.; Ebenstein, Yuval and Tegenfeldt, Jonas O. LU (2016) 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 p.1527-1528
Abstract

Optical mapping relies on the preparation of fluorescent DNA. DNA must be imaged with good signal to noise and therefore the background of unwanted DNA fragments, fluorescent dyes and other reagents need to be removed. We use deterministic lateral displacement to separate 48.5 kbp DNA from < 10 kbp DNA. We also show the removal of 48.5 kbp DNA fragments from a background of fluorescent ATTO647N molecules and the recovery of >50 kbp molecules from a background of shorter digested fragments. In both cases improving signal to noise during imaging.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Deterministic lateral displacement, DNA, Sample cleanup, Separation
in
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
external identifiers
  • scopus:85014224316
ISBN
9780979806490
language
English
LU publication?
yes
id
5c5107bd-979c-4317-bfbb-77db1b7dc61f
date added to LUP
2017-03-16 10:15:36
date last changed
2017-06-30 12:30:59
@inproceedings{5c5107bd-979c-4317-bfbb-77db1b7dc61f,
  abstract     = {<p>Optical mapping relies on the preparation of fluorescent DNA. DNA must be imaged with good signal to noise and therefore the background of unwanted DNA fragments, fluorescent dyes and other reagents need to be removed. We use deterministic lateral displacement to separate 48.5 kbp DNA from &lt; 10 kbp DNA. We also show the removal of 48.5 kbp DNA fragments from a background of fluorescent ATTO647N molecules and the recovery of &gt;50 kbp molecules from a background of shorter digested fragments. In both cases improving signal to noise during imaging.</p>},
  author       = {Kim, Rebekah and Kühnlein, Alexandra and Smith, Darren A. and Ho, Bao Dang and Grunwald, Assaf and Beech, Jason P. and Neely, Robert K. and Ebenstein, Yuval and Tegenfeldt, Jonas O.},
  booktitle    = {20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016},
  isbn         = {9780979806490},
  keyword      = {Deterministic lateral displacement,DNA,Sample cleanup,Separation},
  language     = {eng},
  pages        = {1527--1528},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {DNA sample cleanup using deterministic lateral displacement},
  year         = {2016},
}