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Milk Formula Enriched with Sodium Butyrate Influences Small Intestine Contractility in Neonatal Pigs

Słupecka-Ziemilska, Monika ; Pierzynowski, Stefan Grzegorz LU ; Szczurek, Paulina LU ; Pierzynowska, Kateryna LU orcid ; Wychowański, Piotr ; Seklecka, Blanka ; Koperski, Maciej ; Starzyńska, Anna ; Szkopek, Dominika and Donaldson, Janine , et al. (2022) In Nutrients 14(20).
Abstract

Butyrate, a by-product of gut bacteria fermentation as well as the digestion of fat in mother’s milk, exerts a wide spectrum of beneficial effects in the gastrointestinal tissues. The present study aimed to determine the effects of sodium butyrate on small intestine contractility in neonatal piglets. Piglets were fed milk formula alone (group C) or milk formula supplemented with sodium butyrate (group B). After a 7-day treatment period, isometric recordings of whole-thickness segments of the duodenum and middle jejunum were obtained by electric field stimulation under the influence of increasing doses of Ach (acetylocholine) in the presence of TTX (tetrodotoxin) and atropine. Moreover, structural properties of the intestinal wall were... (More)

Butyrate, a by-product of gut bacteria fermentation as well as the digestion of fat in mother’s milk, exerts a wide spectrum of beneficial effects in the gastrointestinal tissues. The present study aimed to determine the effects of sodium butyrate on small intestine contractility in neonatal piglets. Piglets were fed milk formula alone (group C) or milk formula supplemented with sodium butyrate (group B). After a 7-day treatment period, isometric recordings of whole-thickness segments of the duodenum and middle jejunum were obtained by electric field stimulation under the influence of increasing doses of Ach (acetylocholine) in the presence of TTX (tetrodotoxin) and atropine. Moreover, structural properties of the intestinal wall were assessed, together with the expression of cholinergic and muscarinic receptors (M1 and M2). In both intestinal segments (duodenum and middle jejunum), EFS (electric field stimulation) impulses resulted in increased contractility and amplitude of contractions in group B compared to group C. Additionally, exposure to dietary butyrate led to a significant increase in tunica muscularis thickness in the duodenum, while mitotic and apoptotic indices were increased in the middle jejunum. The expression of M1 and M2 receptors in the middle jejunum was significantly higher after butyrate treatment. The results indicate increased cholinergic signaling and small intestinal growth and renewal in response to feeding with milk formula enriched with sodium butyrate in neonatal piglets.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
cholinergic signaling, intestinal contractility, neonates, pigs, sodium butyrate
in
Nutrients
volume
14
issue
20
article number
4301
publisher
MDPI AG
external identifiers
  • pmid:36296985
  • scopus:85140788860
ISSN
2072-6643
DOI
10.3390/nu14204301
language
English
LU publication?
yes
id
5cf89fdc-9153-4ca8-af2a-60fcdbca1b67
date added to LUP
2022-12-13 15:49:31
date last changed
2024-04-04 14:14:49
@article{5cf89fdc-9153-4ca8-af2a-60fcdbca1b67,
  abstract     = {{<p>Butyrate, a by-product of gut bacteria fermentation as well as the digestion of fat in mother’s milk, exerts a wide spectrum of beneficial effects in the gastrointestinal tissues. The present study aimed to determine the effects of sodium butyrate on small intestine contractility in neonatal piglets. Piglets were fed milk formula alone (group C) or milk formula supplemented with sodium butyrate (group B). After a 7-day treatment period, isometric recordings of whole-thickness segments of the duodenum and middle jejunum were obtained by electric field stimulation under the influence of increasing doses of Ach (acetylocholine) in the presence of TTX (tetrodotoxin) and atropine. Moreover, structural properties of the intestinal wall were assessed, together with the expression of cholinergic and muscarinic receptors (M1 and M2). In both intestinal segments (duodenum and middle jejunum), EFS (electric field stimulation) impulses resulted in increased contractility and amplitude of contractions in group B compared to group C. Additionally, exposure to dietary butyrate led to a significant increase in tunica muscularis thickness in the duodenum, while mitotic and apoptotic indices were increased in the middle jejunum. The expression of M1 and M2 receptors in the middle jejunum was significantly higher after butyrate treatment. The results indicate increased cholinergic signaling and small intestinal growth and renewal in response to feeding with milk formula enriched with sodium butyrate in neonatal piglets.</p>}},
  author       = {{Słupecka-Ziemilska, Monika and Pierzynowski, Stefan Grzegorz and Szczurek, Paulina and Pierzynowska, Kateryna and Wychowański, Piotr and Seklecka, Blanka and Koperski, Maciej and Starzyńska, Anna and Szkopek, Dominika and Donaldson, Janine and Andrzejewski, Krzysztof and Woliński, Jarosław}},
  issn         = {{2072-6643}},
  keywords     = {{cholinergic signaling; intestinal contractility; neonates; pigs; sodium butyrate}},
  language     = {{eng}},
  number       = {{20}},
  publisher    = {{MDPI AG}},
  series       = {{Nutrients}},
  title        = {{Milk Formula Enriched with Sodium Butyrate Influences Small Intestine Contractility in Neonatal Pigs}},
  url          = {{http://dx.doi.org/10.3390/nu14204301}},
  doi          = {{10.3390/nu14204301}},
  volume       = {{14}},
  year         = {{2022}},
}