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Detection of G Protein-Coupled Receptor Autoantibodies in Postural Orthostatic Tachycardia Syndrome Using Standard Methodology

Hall, Juliette ; Bourne, Kate M ; Vernino, Steven ; Hamrefors, Viktor LU orcid ; Kharraziha, Isabella LU orcid ; Nilsson, Jan LU ; Sheldon, Robert S ; Fedorowski, Artur LU orcid and Raj, Satish R (2022) In Circulation 146(8). p.613-622
Abstract

BACKGROUND: Postural orthostatic tachycardia syndrome (POTS) is a disorder of orthostatic intolerance that primarily affects women of childbearing age. The underlying pathophysiology of POTS is not fully understood, but it has been suggested that autoimmunity may play a role. The aim of this study was to compare concentrations of autoantibodies to cardiovascular G protein-coupled receptors between patients with POTS and healthy controls.

METHODS: Sera were collected from 116 patients with POTS (91% female; mean age, 29 years) and 81 healthy controls (84% female; mean age, 27 years) from Calgary, Canada, and Malmö, Sweden. Samples were evaluated for autoantibodies to 11 receptors (adrenergic, muscarinic, angiotensin II, and... (More)

BACKGROUND: Postural orthostatic tachycardia syndrome (POTS) is a disorder of orthostatic intolerance that primarily affects women of childbearing age. The underlying pathophysiology of POTS is not fully understood, but it has been suggested that autoimmunity may play a role. The aim of this study was to compare concentrations of autoantibodies to cardiovascular G protein-coupled receptors between patients with POTS and healthy controls.

METHODS: Sera were collected from 116 patients with POTS (91% female; mean age, 29 years) and 81 healthy controls (84% female; mean age, 27 years) from Calgary, Canada, and Malmö, Sweden. Samples were evaluated for autoantibodies to 11 receptors (adrenergic, muscarinic, angiotensin II, and endothelin) using a commercially available enzyme-linked immunosorbent assay.

RESULTS: Autoantibody concentrations against all of the receptors tested were not significantly different between controls and patients with POTS. The majority of patients with POTS (98.3%) and all controls (100%) had α1 adrenergic receptor autoantibody concentrations above the seropositive threshold provided by the manufacturer (7 units/mL). The proportion of patients with POTS versus healthy controls who fell above the diagnostic thresholds was not different for any tested autoantibodies. Receiver operating characteristic curves showed a poor ability to discriminate between patients with POTS and controls.

CONCLUSION: Patients with POTS and healthy controls do not differ in their enzyme-linked immunosorbent assay-derived autoantibody concentrations to cardiovascular G protein-coupled receptors. These findings suggest that these tests are not useful for establishing the role of autoimmunity in POTS.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Circulation
volume
146
issue
8
pages
10 pages
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:35766055
  • scopus:85136483837
ISSN
1524-4539
DOI
10.1161/CIRCULATIONAHA.122.059971
language
English
LU publication?
yes
id
5d1c8842-2569-4293-9592-b60821be1e23
date added to LUP
2022-07-01 17:39:09
date last changed
2025-10-20 05:59:04
@article{5d1c8842-2569-4293-9592-b60821be1e23,
  abstract     = {{<p>BACKGROUND: Postural orthostatic tachycardia syndrome (POTS) is a disorder of orthostatic intolerance that primarily affects women of childbearing age. The underlying pathophysiology of POTS is not fully understood, but it has been suggested that autoimmunity may play a role. The aim of this study was to compare concentrations of autoantibodies to cardiovascular G protein-coupled receptors between patients with POTS and healthy controls.</p><p>METHODS: Sera were collected from 116 patients with POTS (91% female; mean age, 29 years) and 81 healthy controls (84% female; mean age, 27 years) from Calgary, Canada, and Malmö, Sweden. Samples were evaluated for autoantibodies to 11 receptors (adrenergic, muscarinic, angiotensin II, and endothelin) using a commercially available enzyme-linked immunosorbent assay.</p><p>RESULTS: Autoantibody concentrations against all of the receptors tested were not significantly different between controls and patients with POTS. The majority of patients with POTS (98.3%) and all controls (100%) had α1 adrenergic receptor autoantibody concentrations above the seropositive threshold provided by the manufacturer (7 units/mL). The proportion of patients with POTS versus healthy controls who fell above the diagnostic thresholds was not different for any tested autoantibodies. Receiver operating characteristic curves showed a poor ability to discriminate between patients with POTS and controls.</p><p>CONCLUSION: Patients with POTS and healthy controls do not differ in their enzyme-linked immunosorbent assay-derived autoantibody concentrations to cardiovascular G protein-coupled receptors. These findings suggest that these tests are not useful for establishing the role of autoimmunity in POTS.</p>}},
  author       = {{Hall, Juliette and Bourne, Kate M and Vernino, Steven and Hamrefors, Viktor and Kharraziha, Isabella and Nilsson, Jan and Sheldon, Robert S and Fedorowski, Artur and Raj, Satish R}},
  issn         = {{1524-4539}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{8}},
  pages        = {{613--622}},
  publisher    = {{Lippincott Williams & Wilkins}},
  series       = {{Circulation}},
  title        = {{Detection of G Protein-Coupled Receptor Autoantibodies in Postural Orthostatic Tachycardia Syndrome Using Standard Methodology}},
  url          = {{http://dx.doi.org/10.1161/CIRCULATIONAHA.122.059971}},
  doi          = {{10.1161/CIRCULATIONAHA.122.059971}},
  volume       = {{146}},
  year         = {{2022}},
}