Detection of G Protein-Coupled Receptor Autoantibodies in Postural Orthostatic Tachycardia Syndrome Using Standard Methodology
(2022) In Circulation 146(8). p.613-622- Abstract
BACKGROUND: Postural orthostatic tachycardia syndrome (POTS) is a disorder of orthostatic intolerance that primarily affects women of childbearing age. The underlying pathophysiology of POTS is not fully understood, but it has been suggested that autoimmunity may play a role. The aim of this study was to compare concentrations of autoantibodies to cardiovascular G protein-coupled receptors between patients with POTS and healthy controls.
METHODS: Sera were collected from 116 patients with POTS (91% female; mean age, 29 years) and 81 healthy controls (84% female; mean age, 27 years) from Calgary, Canada, and Malmö, Sweden. Samples were evaluated for autoantibodies to 11 receptors (adrenergic, muscarinic, angiotensin II, and... (More)
BACKGROUND: Postural orthostatic tachycardia syndrome (POTS) is a disorder of orthostatic intolerance that primarily affects women of childbearing age. The underlying pathophysiology of POTS is not fully understood, but it has been suggested that autoimmunity may play a role. The aim of this study was to compare concentrations of autoantibodies to cardiovascular G protein-coupled receptors between patients with POTS and healthy controls.
METHODS: Sera were collected from 116 patients with POTS (91% female; mean age, 29 years) and 81 healthy controls (84% female; mean age, 27 years) from Calgary, Canada, and Malmö, Sweden. Samples were evaluated for autoantibodies to 11 receptors (adrenergic, muscarinic, angiotensin II, and endothelin) using a commercially available enzyme-linked immunosorbent assay.
RESULTS: Autoantibody concentrations against all of the receptors tested were not significantly different between controls and patients with POTS. The majority of patients with POTS (98.3%) and all controls (100%) had α1 adrenergic receptor autoantibody concentrations above the seropositive threshold provided by the manufacturer (7 units/mL). The proportion of patients with POTS versus healthy controls who fell above the diagnostic thresholds was not different for any tested autoantibodies. Receiver operating characteristic curves showed a poor ability to discriminate between patients with POTS and controls.
CONCLUSION: Patients with POTS and healthy controls do not differ in their enzyme-linked immunosorbent assay-derived autoantibody concentrations to cardiovascular G protein-coupled receptors. These findings suggest that these tests are not useful for establishing the role of autoimmunity in POTS.
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- author
- Hall, Juliette ; Bourne, Kate M ; Vernino, Steven ; Hamrefors, Viktor LU ; Kharraziha, Isabella LU ; Nilsson, Jan LU ; Sheldon, Robert S ; Fedorowski, Artur LU and Raj, Satish R
- organization
- publishing date
- 2022-06-29
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Circulation
- volume
- 146
- issue
- 8
- pages
- 10 pages
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- pmid:35766055
- scopus:85136483837
- ISSN
- 1524-4539
- DOI
- 10.1161/CIRCULATIONAHA.122.059971
- language
- English
- LU publication?
- yes
- id
- 5d1c8842-2569-4293-9592-b60821be1e23
- date added to LUP
- 2022-07-01 17:39:09
- date last changed
- 2024-04-19 18:18:26
@article{5d1c8842-2569-4293-9592-b60821be1e23, abstract = {{<p>BACKGROUND: Postural orthostatic tachycardia syndrome (POTS) is a disorder of orthostatic intolerance that primarily affects women of childbearing age. The underlying pathophysiology of POTS is not fully understood, but it has been suggested that autoimmunity may play a role. The aim of this study was to compare concentrations of autoantibodies to cardiovascular G protein-coupled receptors between patients with POTS and healthy controls.</p><p>METHODS: Sera were collected from 116 patients with POTS (91% female; mean age, 29 years) and 81 healthy controls (84% female; mean age, 27 years) from Calgary, Canada, and Malmö, Sweden. Samples were evaluated for autoantibodies to 11 receptors (adrenergic, muscarinic, angiotensin II, and endothelin) using a commercially available enzyme-linked immunosorbent assay.</p><p>RESULTS: Autoantibody concentrations against all of the receptors tested were not significantly different between controls and patients with POTS. The majority of patients with POTS (98.3%) and all controls (100%) had α1 adrenergic receptor autoantibody concentrations above the seropositive threshold provided by the manufacturer (7 units/mL). The proportion of patients with POTS versus healthy controls who fell above the diagnostic thresholds was not different for any tested autoantibodies. Receiver operating characteristic curves showed a poor ability to discriminate between patients with POTS and controls.</p><p>CONCLUSION: Patients with POTS and healthy controls do not differ in their enzyme-linked immunosorbent assay-derived autoantibody concentrations to cardiovascular G protein-coupled receptors. These findings suggest that these tests are not useful for establishing the role of autoimmunity in POTS.</p>}}, author = {{Hall, Juliette and Bourne, Kate M and Vernino, Steven and Hamrefors, Viktor and Kharraziha, Isabella and Nilsson, Jan and Sheldon, Robert S and Fedorowski, Artur and Raj, Satish R}}, issn = {{1524-4539}}, language = {{eng}}, month = {{06}}, number = {{8}}, pages = {{613--622}}, publisher = {{Lippincott Williams & Wilkins}}, series = {{Circulation}}, title = {{Detection of G Protein-Coupled Receptor Autoantibodies in Postural Orthostatic Tachycardia Syndrome Using Standard Methodology}}, url = {{http://dx.doi.org/10.1161/CIRCULATIONAHA.122.059971}}, doi = {{10.1161/CIRCULATIONAHA.122.059971}}, volume = {{146}}, year = {{2022}}, }